Báo cáo khoa học: Temperature and salts effects on the peptidase activities of the recombinant metallooligopeptidases neurolysin and thimet oligopeptidase pdf

Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-speciﬁc alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt

... exons of exons 1a and 1c contributed to the 5¢ nontranslated region of isoform 1 mRNA in which the common exon 2 contains an initiation codon.Intronic 5¢ consensus GT was located adjacent to the 3¢ ... determining their transcription initiation sites. Exon 1b con-tained the initiation codon of isoform 2, and noncoding exons 1a and 1ccontributed to isoform 1 mRNA. The dual luciferase promoter ... signal of KLK12. Two additional initiation siteswere mapped at +2 and +4. This exon ended at +49 and spliced to the common second exon, and was des-ignated exon 1a. The next exon, exon 1b,...

Tài liệu Báo cáo khoa học: "Exploiting Readymades in Linguistic Creativity: A System Demonstration of the Jigsaw Bard" docx

... make the predictions.In the first case, predictions of the pleasantness of stereotype nouns based on the pleasantness of theirsalient properties (i.e., predicting the pleasantness of Y from the ... the scale of the Bard’s genera-tivity, and the quality of its insights.5 Empirical Evaluation The vastness of the web, captured in the large-scale sample that is the Google ngrams, means the Jigsaw ... instances of the patterns“as ADJ and * as” and “as * and ADJ as” fromGoogle, noting the combinations that are found and their frequencies. These frequencies provide linkweights for the Hofstadter-style...

Báo cáo khoa học: Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis docx

... attention. The general architecture of the NPC is evolutionarily con-served in all eukaryotes and consists of ﬁlaments on the cytoplasmic surface and spoke rings and a basket-like structure on the ... Ran. Con-versely, the concentration of RanGDP is high in the cytoplasm as a result of the action of RanGAP, whichpromotes cytoplasmic GTP hydrolysis by Ran in con-junction with RanBP1 and ⁄ ... muscle celldifferentiation.DiscussionIn the present study, we have demonstrated that both the composition and the architecture of NPCs arealtered during the differentiation of C2C12 cells. Ourreal-time...

Báo cáo khoa học: b-Secretase cleavage is not required for generation of the intracellular C-terminal domain of the amyloid precursor family of proteins pot

... S5), and indicate that BACE1is responsible for the generation of at least  20% of APLP2s.BACE1 manipulation alters the quantity and form of APP, APLP1 and APLP2 CTFsTo examine the effects of ... BACE1 on the processing of the APP family of proteins. BACE1 expression regulated the levels and species of full-lengthAPLP1, APP and APLP2, of their shed ectodomains, and of their mem-brane-bound ... the processing of APP, APLP1 and APLP2, and on the production of their ICDs. Wereport that BACE1 KO and overexpression affect the steady-state levels of full-length APLP (FLAPLP) 1 and FLAPLP2...

Báo cáo khóa học: Three cyclin-dependent kinases preferentially phosphorylate different parts of the C-terminal domain of the large subunit of RNA polymerase II potx

... how the phosphorylation of different parts of the CTD contributesto these functions.At least three protein kinases are involved directly in the phosphorylation of the CTD and in the regulation ... only, but the hyper-phosphorylated IIo form was not produced [28]. The authors conclude that the hyperphosphorylation, and the production of the IIo form of pol II and the CTD, is a result of ... graph of the intensity of the signal from the substrates only, we calculated the percentage of signal in the IIo and IIa bands. Thus, assuming that the lower mobilitybands corresponded to the...

Báo cáo khoa học: Sp3 transcription factor is crucial for transcriptional activation of the human NOX4 gene doc

... decreasedby the deletion of GC-box1. The activity was furtherdecreased by the deletion of GC-box2 (Fig. 3B). Asshown in Fig. 3C, the transcriptional activity of the 1 kb of the 5¢-ﬂanking region was ... by the deletion of GC-box3, the GC-richFig. 1. Alignment of the 5¢-nucleotide sequences of the human, rat and mouse NOX4 genes. The numbers on the left are nucleotide posi-tions relative to the ... suppressed the expression of Sp1 and the anti-Sp3 shRNA-expression plasmid suppressed the expres-sion of Sp3. The promoter activity of the NOX4 genewas signiﬁcantly reduced by cotransfection with the anti-Sp3...

Báo cáo khoa học: "Conundrums in Noun Phrase Coreference Resolution: Making Sense of the State-of-the-Art" docx

... 1995)computes the F1 score (harmonic mean) of preci-sion and recall based on the identifcation of uniquecoreference links. We use the of cial MUC scorerimplementation for the two MUC corpora and anequivalent ... measures the impact of each resolution class on the overallMUC score.4.3 ResultsTable 4 shows the results of our resolution classanalysis on the test portions of the six data sets. The # columns ... weighted sum of the observed scores for eachresolution class (i.e. the mean for the class mea-sured on the other ﬁve data sets) weighted by the proportion of CEs that belong to the class. The predicted...

Báo cáo khoa học: Temperature and salts effects on the peptidase activities of the recombinant metallooligopeptidases neurolysin and thimet oligopeptidase pdf

... Temperature and salts effects on the peptidase activities of the recombinant metallooligopeptidases neurolysin and thimet oligopeptidase Vitor Oliveira1, Reynaldo ... TDSÃð3ÞDependence of the hydrolysis reaction ratesby neurolysisn and TOP on concentration and chemicalnature of salts The dependence of the rate constants according to the concentration and the chemical ... dependence upon the hydrolysis of the substrates. The eﬀects of low temperature and high NaCl concen-tration on the hydrolytic activities of neurolysin and TOPdo not seem to be a consequence of large...

Tài liệu Báo cáo khoa học: Temperature and phosphate effects on allosteric phenomena of phosphofructokinase from a hibernating ground squirrel (Spermophilus lateralis) pptx

... Particular attention is paid to the effects of temperature on enzyme allostery and the inﬂuence of temperature in the regulation of PFKactivity at both high (euthermic) and low (hibernating)temperatures.ResultsSelected ... shows the effect of temperature on activation coefﬁcient (Ka) values forallosteric activators and the values of concentration of the inhibitor that reduces control activity by 50% (I50). The ... the effect of phosphate and activation was seen only at low concentrations with amaximal 1.7-fold activation at 2 mm phosphate. AtTable 1. Effects of temperature on the kinetic properties of...

Báo cáo khoa học: Kinetic deuterium isotope effects for 7-alkoxycoumarin O-dealkylation reactions catalyzed by human cytochromes P450 and in liver microsomes Rate-limiting C-H bond breaking in cytochrome P450 1A2 substrate oxidation pdf

... the rate of C-Hbond-breaking is a major factor in determining the rates of the reactions under all conditions. Some shift-ing of the oxidations to the alternate 3-hydroxylationreactions occurs ... steps involved in the generation of the reactive oxygen species, asopposed to the reactions of the activated complex withsubstrates, in light of the low endogenous concentra-tion of NADPH-P450 ... estimatingkcat and Km(Fig. 3). Another possible complication is the oxidation of the product acetaldehyde by P450 2E1[33] and the effect on the apparent kinetic constants,which has not been considered...

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