Báo cáo khoa học: Functional site of endogenous phospholipase A2 inhibitor from python serum Phospholipase A2 binding and anti-in¯ammatory activity ppt

... Functional site of endogenous phospholipase A2 inhibitor from python serum Phospholipase A2 binding and anti-in¯ammatory activity Maung-Maung Thwin1, Ramapatna ... IC50, concentration of the inhibitor that inhibits PLA2 activity by 50%; PIP, phospholipase inhibitor from python; PLI, phospholipase A2 inhibitor; sPLA2, secretory phospholipase A2.Note: ... PLA2 binding, and may be useful as abasis f or the design of novel therapeutic agents.Keywords: anti-in¯ammatory peptide; phospholipase inhibitor from python PIP; protein±protein interaction;phospholipase...

Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... ligand binding, and the role of storage proteins, the cupins constitute one of the mostdiverse superfamilies of proteins known. They havebeen described from all three domains of life [1,2], and usually ... constitute a large and widespread superfamily of b-barrel proteinsin which a mononuclear metal site is both a conserved feature of the struc-ture and a source of functional diversity. Metal -binding residues ... further ligands of Fe2+at estimateddistances of 2.04 A˚ and 2.08 A˚, respectively. The three-histidine Fe2+ site of Bxe _A28 76 is compared to the mononuclear nonheme Fe2+centers of the structurally...

Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... betweenhelices 3 and 4 at the junction crossover – and the unpairing of four base-pairs in the centre of the junctions [19]. Detailedunderstanding of the functional role of these interactionswould ... well-deﬁned dimer. The cata-lytic site consisted of two aspartates ) Asp46 and Asp230 ) which mapped to the catalytic site of RuvC.Protruding from opposite sides of the dimer were twoa-helical ... dimer of Ydc2 while complex II most likelyresults from the binding of two dimers of Ydc2 and its functional signiﬁcance is doubtful [14]. The Ydc2-ND35mutant showed a severely impaired binding...

Báo cáo khoa học: Functional characterization of artemin, a ferritin homolog synthesized in Artemia embryos during encystment and diapause doc

... vitro,an activity characteristic of molecular chaperones and shown here to be sharedby apoferritin and ferritin. This is the ﬁrst report that apoferritin ⁄ ferritinmay protect cells from stress ... denaturation of citrate synthase in vitro,acapability shared by apoferritin and ferritin, and con-fers stress tolerance on transfected mammalian cells.Artemin and ferritin may have arisen from the ... withinnegatively stained particles of artemin indicated thelack of metal storage capacity.Artemin, apoferritin and ferritin inhibit citratesynthase denaturationArtemin, apoferritin and ferritin protected...

Báo cáo khoa học: Functional analysis of the methylmalonyl-CoA epimerase from Caenorhabditis elegans docx

... be built, suggesting conservation of ligand and metal binding sites. Comparison with corresponding sequences from a variety of organisms shows more than 1 ⁄ 6 of the sequence is completely conserved.Mutants ... sequence from P. Sher-manii (MCEE_PROFR) are also labeled.Fig. 6. Model of MCE-1 structure. View along the length of theeight-stranded beta-barrel onto the putative metal binding site invol-ving ... paper we pre-sent a detailed study of the structure and expression of the mce-1 gene in C. elegans.Results and DiscussionIdentiﬁcation and sequence analysis of C. elegans MCESearches in the...

Báo cáo khoa học: Deﬁnition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx

... BakerEN (2003) Structural and functional consequences of binding site mutations in transferrin: crystal structures of the Asp63Glu and Arg124Ala mutants of theN-lobe of human transferrin. Biochemistry ... absence or presence of increasing concentrations of unlabeled Ggly. TheApoTf–Ggly complex was separated from the unreacted Ggly by SDS ⁄ PAGE, and the extent of incorporation of radioactivity was determinedby ... occur.Mutation of the N-terminal or C-terminaliron -binding sites of transferrin does notprevent interaction with GglyN-lobe and C-lobe transferrin mutants were used toinvestigate the effect of loss of...

Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... between the base and substrate -binding amino acid affected the binding of dThd and deoxycytidine (dCyd).ResultsWe performed site- directed mutagenesis of four active site residues of Dm-dNK. Residue ... Structural alignment of wild-type Dm-dNK (blue) (PDB ID:1OE0) and Dm -dNK-E52D (red) illustrating the binding of the feed-back inhibitor dTTP and the position of Mg2+in the active site. L. Egeblad-Welin ... Kirschbaum B &Lofﬂer M (1996) Functional expression of a fragment of human dihydroorotate dehydrogenase by means of thebaculovirus expression vector system, and kinetic inves-tigation of the puriﬁed...

Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot

... levels of aminoacid sequence similarity ranging from 45% to 53%, thearchitecture of the aglycone -binding site of Zm-p60.1differs distinctly from that of Bgl4:1 and its homologs.These ﬁndings offer ... analysis of structural and catalytic prop-erties of the mutants, and simulations of the sub-strate–enzyme interactions of the wild-type and one of the mutants. The results provide indications of ... Agency of the Czech Republic,LC06034, 1M06030, LC06010, MSM0021622415 and MSM0021622412 from the Ministry of Education,Youth and Sports of the Czech Republic, and AV0Z50040507 and AV0Z50040702 from...

Báo cáo khoa học: Functional role of fumarate site Glu59 involved in allosteric regulation and subunit–subunit interaction of human mitochondrial NAD(P)+-dependent malic enzyme pptx

... quantitation of microgram quantities of protein,utilizing the principle of protein-dye binding. AnalBiochem 72, 248–254.38 Segel IH (1993) Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and ... may actas an allosteric inhibitor of m-NAD-ME [18,21,25], and the allosteric properties of this isoform may relateto its particular role in the pathways of malate and glutamine oxidation in ... 1B shows the binding mode of fumarate at the dimer interface. Structural studieshave revealed that Arg67 and Arg91 are the ligandsfor fumarate binding. The side chains of Arg91 and Arg67 form...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... other h and, deletion of up to 273 residues from the C-terminal region of DEC1 (DEC1:1–309, DEC1:1–232 and DEC1:1–139)had little effect on the suppressive activity of DEC1.Expression of these ... resulted inadequate levels of expression. The transcriptional activity of FLAG-BMAL1, and the suppressive activity of FLAG-DEC1, were s imilar to those of intact BMAL1 and DEC1, respectively (Fig. ... suppressive activity of DEC1.Determination of the binding domain of DEC1 to BMAL1The interaction of DEC1 and BMAL1 was previouslydemonstrated by a yeast two-hybrid assay [ 7], and thisinteraction...

Xem thêm