Báo cáo Y học: Modeling the three-dimensional structure of H+-ATPase of Neurospora crassa Proposal for a proton pathway from the analysis of internal cavities pptx

... 2002 Modeling the three-dimensional structure of H+-ATPase of Neurospora crassa Proposal for a proton pathway from the analysis of internal cavities Olivier Radresa1, Koji Ogata2, Shoshana Wodak2, ... putative proton pathway in Neurospora crassa H+-ATPase The chemiosmotic model for PMA1_NEUCR. In the P-type proton pumps, the origin of the transported proton as well as the proton e ntry pathway ... of Neurospora crassa plasma membrane H+-ATPase, using astemplate the 2 .6 A ˚crystal structure of rabbit sarcoplasmicreticulum C a 2+-ATPase [Toyoshima, C., Nakasako, M.,Nomura, H. &...

Báo cáo y học: "Guest Editor’s Editorial: Advances in Managing Hepatitis C Virus (HCV) Infection (A Special Issue)"

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Báo cáo Y học: DNA supercoiling in Escherichia coli is under tight and subtle homeostatic control, involving gene-expression and metabolic regulation of both topoisomerase I and DNA gyrase docx

... 5¢-TCCATAGCAGCGGCGAAACCA-3¢ and chromosomalDNA from strain LM1237 [17] as a template. The PCRfragment was subsequently digested with the enzymes DraIand EcoRV and a 842-bp fragment containing the ... istemperature sensitive, and that the pA1lacO-1 promoterand the lacIq1gene are surrounded by a DNA fragmentoriginating from upstream the gyrA gene and a fragmentcontaining the N-terminal part of ... system(metabolic and hierarchical control analysis) are generallyapplicable. Such quantitative analysis t ools are essential tounderstand the working of the multilayered cell. Recentadvances i n...

Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

... released from transfected COS7 cells by adding heparin to the media, was assayed for catalytic activity. The lipase (A) and esterase (B) activities of the substituted mutants, R40 5A, K41 3A, K41 3A/ K41 4A, ... nm was determined at an excitationwavelength of 386 nm. Oleic acid was used as a standard for free fatty acid.Esterase activity was measured using p-nitrophenylbutyrate as a substrate. Samples ... peak was the same for the wild type and the WW mutant (Fig. 3B).Catalytic activities with long- and short-chainfatty acid substratesTo study the functional impact of the LPL C-terminaldomain...

Tài liệu Báo cáo khoa học: "Modeling the Translation of Predicate-Argument Structure for SMT" ppt

... that not onlyverbal predicates are semantically important,they also form a major part of the sentences.Therefore, whether verbal predicates are trans-lated correctly or not has a great impact ... verbal predicate, the translation of the head word of the argument, as well as the boundarywords of the translation of the argument are used asfeatures.4.3 TrainingTo train the argument reordering ... predicate-argument structure across languages.Predicates1are essential elements in sentences.Unfortunately they are usually neither correctlytranslated nor translated at all in many SMT sys-tems...

Tài liệu Báo cáo Y học: A Raman optical activity study of rheomorphism in caseins, synucleins and tau New insight into the structure and behaviour of natively unfolded proteins pot

... and A5 3T mutants of a- synuclein that cause f amilial c ases of Parkinson's disease.Unfortunately the quality of s ome of these syn uclein ROAspectra i s generally not as good as that of ... were also attempted on a S1-and a S2-casein, these proteins had a tendency to aggregate in the laser beam, which prevented the acquisition of ROA data of suf®cient quality for reliable analysis. ... amyloiddisease [22].MATERIALS AND METHODSMaterials The b-casein w as prepared from whole a cid casein b y the urea fractionation method of Aschaffenburg [35]. The j-casein was prepared by adaptation of...

Tài liệu Báo cáo Y học: Structure of the O-polysaccharide and classiﬁcation of Proteus mirabilis strain G1 in Proteus serogroup O3 potx

... cid(GlcA)andgalacturonicacid(GalA)intheratio 1:5. Analysis on an amino-acid analyser showed the presence o f2-amino-2-deoxygalactose and lysine. The Dconfiguration of GalA and GalN was determined by GLC of ... the acetylated (S)-2-butyl glycosides and the Lconfiguration of lysine by GLC of the acetylated (S)-2-butyl ester. The Dconfiguration of GlcA was established b y a nalysis of 13CNMR chemical ... degrada-tion of P. mirabilis G1 LPS followed by gel-permeationchromatography on Sephadex G-50. Sugar analysis of the polysaccharide after a cid hydrolysis revealed glucuronic a cid(GlcA)andgalacturonicacid(GalA)intheratio...

Tài liệu Báo cáo Y học: Solution structure of the mEGF/TGFa44250 chimeric growth factor doc

... set of solutions reﬂects any uncertainty in the calibration as well as alternative ﬁts to the set of distances. The main advantages of the procedure are simple: the details of the calibration are ... in the starting structure. Although mathemat-ical convergence may be achieved by iterative calculations, the accuracy of the distances obtained remains uncertain.Therefore, we use relaxation ... Typically, however,such calculations generate some nonphysical negative crossrelaxation rates as a consequence of the failure of the approximation of a rigid molecule and because of inaccuracies...

Tài liệu Báo cáo Y học: The solution structure and activation of visual arrestin studied by small-angle X-ray scattering pot

... C-terminaldomains, that produce the dramatically increased afﬁnity for R*; on the other hand, binding of the phosphopeptidemay simply alter the surface features and cause relativelyminor conformational ... implementedin the programKALEIDAGRAPH(v3.08, Synergy Software),setting the molecular mass of the monomer equal to45.3 kDa and the forward scattering of the monomer,I(0)Mto the appropriate value, as ... the activating R175Qmutation [12]. The nature and extent of the conforma-tional change that leads to activation of arrestin is notknown. The situation is complicated by the fact thatvisual...

Báo cáo Y học: Solution structure of the Alzheimer amyloid b-peptide (1–42) in an apolar microenvironment Similarity with a virus fusion domain potx

... was adopted for the elution of the peptide. The fraction containing the pure peptide was lyophilized twiceand the purity assessed by a MALDI-TOF analysis using a Hewlett Packard G202 5A LD-TOF ... bedescribed as a random coil, with only a small population of local nonrandom structures. Overall, these data indicatethat bulk water is not suitable for high resolution conform-ational analysis of Ab. ... stronglyhydrophobic side chains; this feature has been aptlydescribed by Rajan et al. as a Teﬂon coating that cansurround a helix [16] in the case of a mixture of water andhexaﬂuoroacetone hydrate,...

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