Báo cáo khoa học: The phosphorylation pattern of human as1-casein is markedly different from the ruminant species potx

Báo cáo khoa học: Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus docx

Báo cáo khoa học: Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus docx

... increased by the addition of 2mm CoCl 2 (relative activity to that of the standard reaction 170%) and not by the addition of 2 mm ZnCl 2 or one of the other metals ⁄ salts tested. The enzyme was ... this indicates that the metal ion is essential for activity, further research is needed to establish if the zinc atom is catalytic or structural. This has been done for...
Ngày tải lên : 16/03/2014, 14:20
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Báo cáo khoa học: Expression and characterization of recombinant 2¢,5¢-oligoadenylate synthetase from the marine sponge Geodia cydonium ppt

Báo cáo khoa học: Expression and characterization of recombinant 2¢,5¢-oligoadenylate synthetase from the marine sponge Geodia cydonium ppt

... that the addition of the nuclease caused the precipitation of the material in a concentration-dependent manner. Formation of the precipitate in the solution containing the highest amount of the ... different sponge cDNA constructs were chosen for studies investigating whether modification of either the N- or C-terminus of the protein could affect the propert...
Ngày tải lên : 23/03/2014, 09:20
  • 13
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Báo cáo khoa học: Mechanisms and kinetics of human arylamine N-acetyltransferase 1 inhibition by disulfiram potx

Báo cáo khoa học: Mechanisms and kinetics of human arylamine N-acetyltransferase 1 inhibition by disulfiram potx

... than the reaction of cisplatin with the enzyme, thus supporting the suggestion that NAT1 could be an in vivo target of DS. The human NAT2 isoform shares a similar structure and mecha- nism of action ... thus be inhibited by the DS-depen- dent formation of an intramolecular mixed disulfide [3]. Therefore, the DS-dependent inhibition of NAT1 is probably the result of...
Ngày tải lên : 07/03/2014, 02:20
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Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc

Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc

... rather reflects synchrony to other events of the spermatogenesis. For instance, the mech- anisms unleashing the pass of the haploid germ cells through the tight junctions from the adluminal to the luminal ... spectrum of tissues but restric- tion to particular cell types and populations. The absence of signal in regions of the nervous system in the human cDNA...
Ngày tải lên : 16/03/2014, 23:20
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Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt

Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt

... sequence is because of the proven efficacy of the designed PNA to down-regulate the gene expression of the mutated allelle, while sustaining the transcription of the wild-type allele [29,29a]. The present ... summary, the present study offers a possible stereochemical basis for the experimentally observed highly diminished stability of the PNAÆDNA duplex in the...
Ngày tải lên : 07/03/2014, 21:20
  • 16
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Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf

Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf

... That the KIR is mostly unstructured when SOCS3 is in isola- tion is perhaps not surprising in view of the hypothesis for its mechanism proposed by Yasukawa et al. [6]. This requires the KIR to ... mutation. The PEST sequence identified in SOCS3 does not occur, according to sequence analysis, in the same location in any other members of the SOCS family, apart from CIS. A...
Ngày tải lên : 16/03/2014, 14:20
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Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx

Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx

... that the primary effect of this mutation is not on the catalytic activity of the enzyme. Absorption spectra of the purified mutant proteins To assess whether these mutations affected the affinity of ... present on the west- ern blot and by the minimal effect of chaperonin (Fig. 1B). This suggests that the R256T mutation harms the function of the folded protein...
Ngày tải lên : 20/02/2014, 02:21
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Tài liệu Báo cáo khoa học: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa Comparison of twelve xylanases in relation to their thermal stability pdf

Tài liệu Báo cáo khoa học: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa Comparison of twelve xylanases in relation to their thermal stability pdf

... part of the C-terminal tail of the full-length NFX with total 301 residues. The amino acids of the C-terminal tail were sticking out from the model, but if the C-terminus of NFX is excluded, the enzyme ... [39]. These results showed that the introduction of five arginines into the Ser/Thr surface increased considerably the thermotolerance in the presence of...
Ngày tải lên : 20/02/2014, 23:20
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Tài liệu Báo cáo khoa học: Multiple enzymic activities of human milk lactoferrin ppt

Tài liệu Báo cáo khoa học: Multiple enzymic activities of human milk lactoferrin ppt

... that these five activities are associated with different isoforms of LF and that, in addition to the three previously reported isoforms [9], one of which is LF RNase, further isoforms of this protein ... Thedatashowtherelative activity of different subfractions of LF from the milk of one donor (Fig. 2) and the range of variation in the relative activities of LF sub...
Ngày tải lên : 21/02/2014, 00:20
  • 9
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Báo cáo khoa học: Folding and turnover of human iron regulatory protein 1 depend on its subcellular localization pdf

Báo cáo khoa học: Folding and turnover of human iron regulatory protein 1 depend on its subcellular localization pdf

... cluster assembly machinery of yeast mitochondria can build the inorganic unit of this normally cytosolic protein. Furthermore, members of the extra-mitochondrial assembly line of these cofactors, the so-called ... cluster biosynthesis has recently been the topic of intense interest, particularly in S. cerevisiae. An extensive set of proteins participa- ting in different...
Ngày tải lên : 07/03/2014, 10:20
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