... increased by the addition of
2mm CoCl
2
(relative activity to that of the standard
reaction 170%) and not by the addition of 2 mm
ZnCl
2
or one of the other metals ⁄ salts tested. The
enzyme was ... this indicates
that the metal ion is essential for activity, further
research is needed to establish if the zinc atom is
catalytic or structural. This has been done for...
... that the addition of the
nuclease caused the precipitation of the material in a
concentration-dependent manner. Formation of the
precipitate in the solution containing the highest
amount of the ... different sponge cDNA constructs were
chosen for studies investigating whether modification
of either the N- or C-terminus of the protein could
affect the propert...
... than the reaction of cisplatin
with the enzyme, thus supporting the suggestion that
NAT1 could be an in vivo target of DS. The human
NAT2 isoform shares a similar structure and mecha-
nism of action ... thus be inhibited by the DS-depen-
dent formation of an intramolecular mixed disulfide
[3]. Therefore, the DS-dependent inhibition of NAT1
is probably the result of...
... rather reflects synchrony to other
events of the spermatogenesis. For instance, the mech-
anisms unleashing the pass of the haploid germ cells
through the tight junctions from the adluminal to the
luminal ... spectrum of tissues but restric-
tion to particular cell types and populations. The
absence of signal in regions of the nervous system in
the human cDNA...
... sequence is because of the proven
efficacy of the designed PNA to down-regulate the
gene expression of the mutated allelle, while sustaining
the transcription of the wild-type allele [29,29a]. The
present ... summary, the present study offers a possible
stereochemical basis for the experimentally observed
highly diminished stability of the PNAÆDNA duplex in
the...
... That the
KIR is mostly unstructured when SOCS3 is in isola-
tion is perhaps not surprising in view of the hypothesis
for its mechanism proposed by Yasukawa et al. [6].
This requires the KIR to ... mutation.
The PEST sequence identified in SOCS3 does not
occur, according to sequence analysis, in the same
location in any other members of the SOCS family,
apart from CIS. A...
... that the primary
effect of this mutation is not on the catalytic activity
of the enzyme.
Absorption spectra of the purified mutant
proteins
To assess whether these mutations affected the affinity
of ... present on the west-
ern blot and by the minimal effect of chaperonin
(Fig. 1B). This suggests that the R256T mutation
harms the function of the folded protein...
... part of the C-terminal tail
of the full-length NFX with total 301 residues. The amino
acids of the C-terminal tail were sticking out from the
model, but if the C-terminus of NFX is excluded, the
enzyme ... [39]. These results showed that the introduction of five
arginines into the Ser/Thr surface increased considerably the
thermotolerance in the presence of...
... that these
five activities are associated with different isoforms of LF
and that, in addition to the three previously reported
isoforms [9], one of which is LF RNase, further isoforms of
this protein ... Thedatashowtherelative
activity of different subfractions of LF from the milk of one donor (Fig. 2) and the range of variation in the relative activities of LF sub...
... cluster
assembly machinery of yeast mitochondria can build
the inorganic unit of this normally cytosolic protein.
Furthermore, members of the extra-mitochondrial
assembly line of these cofactors, the so-called ... cluster biosynthesis has
recently been the topic of intense interest, particularly
in S. cerevisiae. An extensive set of proteins participa-
ting in different...