... First evidence of catalytic mediation by phenolic compounds
in the laccase-induced oxidation of lignin models
Francesca d’Acunzo and Carlo Galli
Dipartimento ... (undesired
routes). In this study, we first investigated the kinetics of
oxidation of phenol red by Poliporus pinsitus laccase in the
absence of any mediator, and then evaluated the ab...
... to
clarify whether the stoichiometry of the c rings is
in uenced by variation of the expression level of
subunit c.
In the present study we investigated whether the
c subunits from the sodium F-ATP ... the
diameter of the c rings may be determined by the struc-
ture of the c monomer and not by the number of assem-
bled subunits. This finding is in a...
... and ERb was
increased in both ERE1 and ERE2 in the presence of
E2. Interestingly, the kinetics of E2-dependent binding
of ERa and ERb to both ERE1 and ERE2 are differ-
ent. The binding of ERa is ... absence of E2, and this binding was
increased in the presence of E2 (Fig. 5A,B; compare
0 h and 6–8 h time points). These differences in the
kinetic profiles of bind...
... cycles in such a combination
do not touch each other). The value (the sign) of a cycle is
the product of the values (the signs) of its steps. The sign o f
the magnitude of a Ôminus combined autoinfluence ... negative in the characteristic equation
(a positive autoinfluence). The absolute magnitude of a
combined autoinfluence path is the product of all the rate...
... quantified by
phosphorimaging of the band corresponding to the full-
length protein, and normalizing to the Coomassie blue
staining of the same band.)
It was next demonstrated that the kinase eluted in
fraction ... aldosterone and insulin, as well as
intracellular signalling components such as protein kinase C
and protein kinase A, were found to increase the in vivo
phosp...
... localized in sucrose gradients by
measuring the A
280
of fractions.
p26 induced thermotolerance in E. coli
Transformed E. coli, incubated overnight with shaking at
37 °C in 2 mL of LB medium, containing ... resem-
blance, and the functional analysis mentioned previ-
ously, indicate data obtained by examining bacterially
produced p26 are indicative of the protein synthesi...
... found in the FAD49 PX
domain, as in many PX domains, suggesting that the
PX domain could bind to an SH3 domain of FAD49.
To test whether the PX domain could interact with an
SH3 domain in FAD49, ... PX
domain to phosphoinositides might be regulated by the
interaction between the PX domain and the third SH3
domain, as in p47
phox
, although whether the interaction
of...
... Mutations in
the 23S ribosomal RNA in the vicinity of the double hook
binding or in the b-hairpin of protein L22 were shown to
bypass the elongation arrest [72].
The swing of protein L22 hairpin tip ... and the L22 double
hook should trigger the swing of the entire b-hairpin tip, in a
manner similar to that caused by TAO binding. As a result
of the swin...
... molecules as
stimuli of Ca
2+
in ux and signalling in yeast is of particular
interest. The authors report that these compounds repre-
senting intermediates of both sphingolipid biosynthesis and
degradation ... Li
+
ion
extrusion from the cell. We therefore investigated whether
the loss of halotolerance in isc1D cells was due to the failure
of ENA1 induction in the...
... chain
corresponding to the catalytic domain (C domain);
and the B chain corresponding to the translocation
domain (T domain) and the receptor-binding domain.
The C and T domains remain covalently linked by ... of the pro-
tein in the N state and the evolution of the overall
structure of the protein in the MG state. This degree
of resolution is unprecedent...