Báo cáo khoa học: Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major doc
Tài liệu Báo cáo khoa học: Purification and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc
... CLGAT 3a (5¢-GCTGTGGCTACGAATGAGGCCGCC-3) and CLGAT3b(5¢-AAGGGAGAGAAACCTGACCTTGCACTTG-3¢). The 5¢-RACE was performed using Marathon cDNA AmplificationKit (Clontech, Palo Alto, CA) according to the manufacturer’sinstructions, ... (1986)Biosynthesis and metabolism of arginine in bacteria.Microbiol Rev 50, 314–352.10 Davis RH (1986) Commpartmental and regulatorymechanisms in the arginine pathways of Neurosporacrassa and Saccharomyces ... synthase; AGK, N-acetylglutamate kinase; AGPR,N-acetylglutamate 5-phosphate reductase; AOAT, N-acetylornithinetransaminase; GAT, glutamate N-acetyltransferase; AOD, N-acetyl-ornithine deacetylase;...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... 2000-fold increase in specificactivity. Analysis of the lectin on SDS/PAGE gave a singleband at apparent molecular mass of 34 kDa. The binding affinity of the lectin in the hemolymph of the freshwater ... NaCl/Triscontaining 1MNaCl and 10 mm CaCl2at 4 °C, until the A 280 of the effluent was 0.002. The column was transferred to a water bath at32 °C and washed with NaCl/Tris containing 300 mm NaCl until the A 280 of ... Bovine submaxillary mucin,which contains mainly 9-O-acetyl- and 8,9 di-O-acety-N-acetyl neuraminic acid was the most potent inhibitor of the lectin. Sialidase treatment and de-O-acetylation of...
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Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... behavior istypical of integral membrane proteins. The polypeptide with anapparent molecular mass of 53 kDa appears as a double band in unboiled samples (lanes A1 and B1).Table 1. N-Terminal ... major polypeptide bands with apparentmolecular masses of 53, 34, 31, and 16 kDa after SDS/PAGE (Fig. 1, lane A1 ). In the boiled sample, the 34-kDapolypeptide was only detectable at lower intensities. ... cofactor-containing proteins translocatedinto the periplasm via the Tat translocase [27]. As deducedfrom the N-terminal sequence of AF499, the signal peptide is not present in the mature enzyme (Table...
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Báo cáo khoa học: Purification and characterization of zebrafish hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot
... lessthan that of MHCE because a considerable amount of undigested bands remained in the digest after a 10 minincubation, and the 36.5 kDa band was not easilydetected even after a 120 min incubation. ... either by ZHE1, MHCE orMLCE, and their SDS ⁄ PAGE patterns were com-pared. SDS ⁄ PAGE of the MHCE digest after a 10 and 40 min incubation gave two bands (43 and 39 kDa), and an additional band ... SDS ⁄ PAGE of the active fraction gave a singleband, with an estimated molecular mass of 23 kDa(Fig. 3). A partial amino acid sequence from the N-ter-minus of the 23 kDa protein was NALIXE,...
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Báo cáo khoa học: Purification and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt
... translation showed that they hold the characteristic features of all known papain-class cysteine proteinases, and a phylogenetic analysis revealed the existence of several papain and chymopapain ... proteinases it can be deduced that the commonancestor of the genera Carica and Vasconcelleaalready contained at least two different cysteine pro-teinases in its latex (papain and chymopapain). ... CC-Ia and CC-Ib confirmed theirequivalence with papain. Based on the observation thatCC-I and papain have catalytic constants of the sameorder of magnitude on BAPNA and chromozyme [17], the marked...
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Báo cáo khoa học: Purification and characterization of cathepsin B-like cysteine protease from cotyledons of daikon radish, Raphanus sativus docx
... Kentaro Ogawa, Hiroko Saika, Keizo Yuasa and Masami NagahamaDepartment of Biological Science and Technology, University of Tokushima Graduate School, JapanGenome analysis has indicated that plants, ... 5¢-ATGGCTGTTTACAATACCAAACTCTG-3¢,corresponding to the N-terminal sequence (Met1–Cys9) of Arabidopsis CBCP, and the antisense primer5¢-CAGCACCAAATGCCCAGCAAGAACC-3¢, corres-ponding to the internal ... cotyledons obtained 8 days afterimbibition. From cotyledons obtained 3 days afterimbibition, a faint 28 kDa band and additional bandswere also detected. A clear 28 kDa band and addi-tional bands were...
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Báo cáo khoa học: Purification and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily doc
... 4).Activities of purified arginase and H. pyloriarginase-containing extracts with divalent cationsMammalian arginases require manganese for optimalcatalytic activity, although some of these arginases ... that RocF is neces-sary and sufficient for arginase activity, and that the enzymehas a number of interesting and unique features among the arginase superfamily, including optimal enzymatic activitywith ... at )20 °C until measurement of arginase activity.No arginase activity was detected in the correspondingpellets.Arginase activity assay The colorimetric arginase assay measures the amount of ornithine...
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Báo cáo khoa học: Purification and characterization of NTPDase1 (ecto-apyrase) and NTPDase2 (ecto-ATPase) from porcine brain cortex synaptosomes doc
... 2000times in Tris-buffered saline. After several washes, the membrane was incubated with alkaline phosphatase conju-gated goat anti-rabbit Igs at a dilution of 1 : 30 000. The bands were visualized ... acid [30] using bovine serum albumin as a standard.ResultsIsolation and purification Polydocanol at a concentration of 0.9% released 70% of ATP/ADPase active protein from purified synaptic3448 ... ACR domains are thought to play a critical role in the binding and hydrolysis of substrates as site directedmutagenesis within these domains lead to the loss of biological activity of NTPDases...
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Báo cáo khoa học: Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major doc
... Iijima et al.(Eur. J. Biochem. 270) Ó FEBS 2003 Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major Noriaki ... Immunohistochemical localization of chrysophsins in the gills of the red sea bream. Adjacent sections in the gills were immunostainedwith chrysophsin-1 antibody (A, C, E and F) and with hematoxylin and eosin ... isolation of three isoforms of a novel C-terminally amidated peptide from the gills of red sea bream, Chrysophrys (Pagrus) major. Peptide sequenceswere determined by a combination of Edman degrada-tion,...
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Báo cáo khoa học: Purification and characterization of novel salt-active acharan sulfate lyase from Bacteroides stercoris HJ-15 ppt
... lyase; acharan sulfate lyase; acharan sulfate; heparin.Heparin, heparan sulfate and acharan sulfate glycosamino-glycans (GAGs) are comprised of alternating 1–4-linkedglucosamine and uronic acid ... by the milligrams of protein in the cuvette. Protein concentra-tion was measured by a Bradford assay using bovine serumalbumin as a standard [18]. Characterization of salt-active acharan sulfate ... 3171Substrate specificity of purified salt-active acharansulfate lyase The purified salt-active acharan sulfate lyase degradedheparin and heparan sulfate as well as acharan sulfate(Table 5). The salt-active...
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