... osmotic sorbitolgradient of 300 mosmÆkg)1 in a rapid mixing device.The resulting cell shrinkage was determined by moni-toring the relative increase in light scattering (Fig. 3A,left panel). ... structure of Thr, which can interfere withprotein function [28,29]. The branching next to the car-bon atom carrying the amino group may clash with thedense packing in the NPA protein region. Putting ... analysis of theexpression levels in yeast, equal amounts of total proteinwere separated by SDS ⁄ PAGE, checked for even loading of the lanes by Coomassie Blue staining, blotted ontopoly(vinylidene...