... preparation).
Thus it is unlikely that the regulation of protein synthesis is
simply a consequence of either the impairment of cell cycle
progression or the induction of apoptosis, both of which are
associated ... does not
reverse the inhibition of protein synthesis. We conclude that
the p53-regulated cleavages of eIF4GI and eIF4B, as well as
the overall inhibitio...
... acti-
vation of a cryptic splice site is a common cause of
genetic disease. We recently reported the example of a
deep intronic mutation c.31-279A>G in the NF1 gene
of a patient with a severe form of ... exclusion. However,
given that the m4 motif is immediately adjacent to the
5¢ splice site, it is very likely that this mutation is
involved in recognition of this seque...
... finger is dispensable [28]. Another interesting
example is provided by Pirh2, a p53-induced RING
finger E3 ligase promoting ubiquitination and degrada-
tion of p53; very recently, isoforms of Pirh2 ... examples of RING E3s, the activity of which
critically depends on multiprotein complexes, including
homo- or hetero-oligomers of RING finger proteins.
Of note, interaction betwee...
... functionally an-
notated protein sequences. The iProClass database is
a central point for exploration of protein information,
which provides summary descriptions of protein fam-
ily, function ... repository of
protein sequence and annotation created by joining
Swiss-Prot, TrEMBL, and PSD. There are three
knowledge components in UniProt: Swissprot,
TrEMBL, and UniRef. Swis...
... and the
other of residues from the SET-C and post-SET
regions. Lysine 4 of histone H3 is inserted into a chan-
nel, at the end of which is the AdoHcy binding site,
which is composed of residues ... [35]. This analysis reveals that the iso-
lated MLL1 SET domain is a relatively slow H3K4
monomethyltransferase, which is consistent with the
predictions of the Phe ⁄ Tyr switch...
... reduction of the level of cell surface expres-
sion of this mutated version of MT1-MMP. It is
important to note that perturbation of the GRASP55
interaction with TGF-a [37], as well as p24 proteins
[39], ... substrates. So far, it is unknown whether this
mechanism is specific for the type I transmembrane
MT-MMPs or whether it comprises a more general
mechanism for furin-mediate...
... levels of secondary
structure and disrupted tertiary structure [33,34].
Discussion
According to the prion hypothesis, a key molecular
event in the pathogenesis of prion diseases is the
conversion of ... molecular event in prion diseases is the conversion of the cellular
conformation of the prion protein (PrP
C
) to an altered disease-associated
form, generally denoted as scrap...
... members of the Ras family of small G proteins.
The activity of Ras proteins is under tight control of
several classes of guanine nucleotide exchange factors
(GEFs) and GTPase-activating proteins. ... Furthermore,
Ras-GRF1 is also heavily phosphorylated upon agon-
ist activation of GPCRs, but the exact role of these
phosphorylations is not fully understood. Protein kin-
a...
... mobility of helix A of the
protein. As Trp13 is located in the vicinity of the activation
region AR2 of the protein [1], which is responsible for the
activation of the second class of E. coli ... correlation time of Trp85 in apo-CRP of 20.5 ns
indicates that this residue is immobilized within the
N-terminal domain of the protein and exhibits motion
characteristic...
... rate of protein
synthesis, mutations and aberrant protein biogenesis,
environmental (usually heat or oxidative) stress and
heterologous protein production.
Ageing is mostly known to induce protein ... production is by itself cause of
toxicity for cells, independently of the nature of the
recombinant protein. Energy depletion is the most
immediate result and is due to...