... 5¢-triphosphate
synthase. Identification of residues required for CTP formation
and GTP-dependent activation of glutamine hydrolysis
Dave Simard, Kerry A. Hewitt, Faylene Lunn, Akshai Iyengar and Stephen L. ... domain and wild-type ability to
catalyse NH
3
-dependent CTP formation. For these mutants,
the values of k
cat
/K
m
and k
cat
for glutamine-...
... (dFBA), and was
applied for diauxic growth of E. coli on glucose and
acetate [8]. The model predicts very well the time
course of the external metabolites and the growth of
biomass. In Santillan and ... system in Escherichia coli is a transport and sen-
sory system and, in this function, is one of the key players of catabolite
repression. Mathematical modelling o...
... biological molecules. Knowledge of the
conformational properties of EspB may clarify the role
of EspB in bacterial attachment, but no information
about the structural properties of EspB is currently
available.
In ... less organ-
ized conformations of EspB and IpaC may be a com-
mon property for this class of proteins.
Importantly, some proteins that are natively unfol-
ded sho...
... observed that both T-pIC and
M-pIC treatments induced the phosphorylation at
Ser386 and Ser396 of IRF-3, and formed the dimeriza-
tion of IRF-3 (Fig. 5A,B, lanes 1 and 2), and that
NS3-4A remarkably ... Grants-in-Aid for the Third-Term Comprehensive
10-Year Strategy for Cancer Control, and by a
Grant-in-Aid for Research on Hepatitis, both from the
Ministry of Health, L...
... with (i) a mixture of A115 and F23, (ii) a mixture of A115 and F23, (iii) Y353 and (iv) A115.
Table 5. Kinetic and stability parameters for purified WT and mutant CPUs. The T1 ⁄ 2 of activity at 37 ... in its name stands for unsta-
ble [10,11]. The inherent and irreversible decay of
CPU’s activity is believed to be of major importance
for its in vivo down-regulation...
... bond
(Arg193–Ser194) and a disulfide bridge (Cys186–
Cys201). Proteolytic nicking of the polypeptide and
reduction of the disulfide are required for the A and B
fragments to separate and for cytotoxicity ... mobilities of the immunoreactive proform ( 64 kDa) and mature form ( 45 kDa) of cathepsin D, and soluble trun-
cated furin ( 70 kDa). (B) Eluted fractions (5, 7...
... which aligns with His200
of the d
1
heme domain of cd
1
, is essential both for this binding and for the
production of d
1
heme; replacement of His41 by Ala, Cys, Lys and Met all
gave nonfunctional ... information (Doc. S1, S2 and S3).
Cloning of P. pantotrophus nirF and nirF variants
The nirF ORF was amplified from P. pantotrophus genomic
DNA using SB5 and SB6, digeste...
... high-affinity
binding of Man-6-P-bearing ligands, but possibly not
for IGF-II. The present study, employing soluble
forms of the receptor [25,26,34], indicates that the
ectodomain of the receptor is capable of ... ectodomain of these heterodimers,
resulted in the predicted amount of ligand binding.
This loss of binding function observed with asym-
metric heterodimers may be due...
... interact with two
types of collagen I found in vivo, and the form of the
collagen determines the cells synthesis and activation
of MMP-2 as well as the synthesis of MT1-MMP and
TIMP-1.
Previously, ... which
synthesis and activation of proMMP-2, as well as syn-
thesis of MT1-MMP, TIMP-1 and TIMP-2, are
affected by the interaction of the cell with various bio-
logica...
... the
intermediate and mature forms of Toc75 in a given
supernatant or pellet fraction showed a similar pattern
of protease sensitivity (e.g., compare lanes 4 and 6, or
27 and 29 of Fig. 2A) as was shown before ... for
AGA, compare lanes 19 and 20, 23 and 24, 27 and 28,
and 31 and 32, respectively). Furthermore, trypsin-sen-
sitivity of imported proteins was also consi...