Báo cáo khoa học: The antibiotic activity of cationic linear amphipathic peptides: lessons from the action of leucine/lysine copolymers on bacteria of the class Mollicutes doc

... antibiotic activity of cationic linear amphipathic peptides: lessons from the action of leucine/lysine copolymers on bacteria of the class Mollicutes Laure Be´ven1, Sabine Castano2, Jean Dufourcq2,A˚ke ... Hence, the anti-bacterial action of these peptides comprises four main steps:step 1, interfacial partitioning and exofacial accumulation of Fig. 5. Graphical illustration of the eﬀects on antibacterial ... check whether these rules hold also for the antimicrobial activity of these peptides. In this work, wehave taken advantage of the fact that bacteria of the class Mollicutes are devoid of an outer...

Báo cáo khoa học: "A DOM Tree Alignment Model for Mining Parallel Data from the Web" doc

... in the context of language generation, while we search for the best alignment on the condition that both trees are given. To facilitate the presentation of the tree align-ment model, the ... Precision 93.5% 97.2% Table 1: Precision of Mined Parallel Documents The document-level mining precision solely depends on the candidate document pair verifica-tion module. The verification ... verification. Based on these mining results, the quality of the mined data, the mining coverage and mining efficiency are measured. First, we benchmarked the precision of the mined parallel documents....

Báo cáo khoa học: "Unsupervised Relation Extraction by Mining Wikipedia Texts Using Information from the Web" pdf

... sequence of words. The distance of two surface patterns is deﬁned as the fraction of the LD value to the length of the longer sequence.For estimating the number of clusters k, we ap-ply the stability-based ... good precision usinglinguistic patterns, whereas surface patterns from Web frequency information contributegreatly to the coverage of relation extraction.• The combination of these patterns ... ad-1022dition to the article texts.To establish the background for this paper, westart by deﬁning the problem under consideration:relation extraction from Wikipedia. We use the en-cyclopedic...

Báo cáo khoa học: Structure–cytotoxicity relationships in bovine seminal ribonuclease: new insights from heat and chemical denaturation studies on variants ppt

... a comparison of the thermodynamic and chemical stability of the NCD form of BS-RNase with that of the monomeric derivative,together with an investigation of the thermal dissociation mechanismrevealing ... stabilities,indicating that the substitution of four residues in the hinge region does not signiﬁcantly perturb the globalstability of the monomeric form of BS-RNase. On the other hand, the single mutation R80S ... those of the respective monomers. This indicates a loss of interac-tions of the monomers in the dimeric structures. Inconclusion, all of the reported data indicate that the R80S mutation is...

Báo cáo khoa học: Broad antibiotic resistance proﬁle of the subclass B3 metallo-b-lactamase GOB-1, a di-zinc enzyme potx

... below). Therefore, the mutation of the glutamine residue at position 116 results in the loss of zinc from the corresponding site of the enzymeunder MS conditions. Q116H, like the wild-type, con-tains ... that the activeform of the enzyme contains only one zinc ion, locatedin the DHH site. This is in contrast to all known B1and B3 MBLs, with the possible exception of the mono-Co++form of BcII ... varying EDTA concentrations (0.5–50 lm), in the same buffer as that used for the other kinetic experiments,without the addition of zinc. The dependence of ki on the concentration of chelating...

Báo cáo khoa học: Structure and activity of the atypical serine kinase Rio1 doc

... levels of fractions from reverse-phase HPLC separation of the tryp-tic peptides from autophosphorylatedAfRio1. (C–E) Edman degradation of pep-tides obtained from the radioactive fraction of HPLC ... whichmarks the start of aC. The entire ﬂexible loop packsbetween the N-terminal portion of aC and part of the C-lobe (Figs 2A and 3A). The interactions between the ﬂexible loop and the rest of the ... hingeregion, one from the peptide carbonyl oxygen of con-served Glu148 to the amino group N6, and one from the peptide amine of Ile150 to the indole nitrogen N1. The ribose moiety is contacted...

Tài liệu Báo cáo khoa học: Multi-targeted activity of maslinic acid as an antimalarial natural compound pdf

... effect on the functionality of the vacuole as a result of the accumulation of partiallyhydrolysed peptides, leading to the accumulation of uncleaved globin [39]. The possible effect, either director ... subtracted from the inset data. Plots of percent-age control activity versus concentration of inhibitor wereused to determine the concentrations that inhibited 50% of protease activity. The enzymes ... in the processing of apicoplast protein leader sequence.Inhibition of any of these activities could contribute toMA interference in the maturation of the parasite.Polypharmacology of MAThe...

Tài liệu Báo cáo khoa học: Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping ppt

... twodistinct functions of TorD with the folding of TorAand possible interactions of both proteins with the MGD biosynthetic apparatus [12]. The concentration of GTP in the cytoplasm of an exponentially ... example, the GTPase activity of TorD is dependent upon the presence of magnesiumions and this is clearly in line with the requirements of the classical GTPases [29,30]. Magnesium often func-tions ... level of GTPase activity, and also require the action of GTPase activating proteins (GAPs), or the interactionwith a speciﬁc effecter, to catalyse the hydrolysis of GTP. The transition from GTP- to...

Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx

... stabilize the oxyani- on that develops during cleavage of the peptide bond.In contrast, the c-amide hydrogen of the Asn164 resi-due of E78H ⁄ D164N may participate in stabilization of the transition ... by the addition of the enzyme (up to50 lL). After incubation for 10 min, the reaction wasstopped by the addition of 300 lL1m Tris ⁄ HCl, pH 9.5; the mixture was then immediately chilled on ... full-length precursorand the ligation reaction was at equilibrium under the conditions. The rate of the formation of the full-lengthprecursor was not enhanced when the nicked precursorwas incubated...

Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc

... analysis, and the determination of released ammonia. The coupled enzymeassay revealed the mechanism of the deamination reactionand the subsequent metabolism, including the deaminationstep. The product ... characterization of the purified enzyme The c oncentrated enzyme solution (fraction 6) was yellow i ncolor. The enzyme s olution showed one main absorptionpeak at 266 nm and a broad absorption band in the ... Purification of the 2-amino-5-carboxymuconic 6-sem ialdehyde deaminase f rom Bordetella sp. strain 10d. Fractions 1–6 refer t o the fractionsobtained at the en d of ste ps 1–6 of th e purification proc...

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