Báo cáo khoa học: Cytochrome P460 of Nitrosomonas europaea Formation of the heme-lysine cross-link in a heterologous host and mutagenic conversion to a non-cross-linked cytochrome c ¢ pot

... were 5¢- GTAACTGTAAGAGAACTGGTCAC- 3¢ (Lys70 to Arg), 5¢- GTAACTGTAGCAGAACTGGTCAG- 3¢ (Lys70 to Ala), and 5¢- GGTAACTGTATATGAACTGGTCAG- 3¢ (Lys70 to Tyr). The resulting plasmids,pUCYPKR, pUCYPKA, and ... Cytochrome P460 of Nitrosomonas europaea Formation of the heme-lysine cross-link in a heterologous host and mutagenic conversion to a non-cross-linked cytochrome c ¢ David J. Bergmann1 and Alan ... subcloned into the plasmid vector pUC119 [16]. The forward primer, 5¢- GCTACCATATGAAAACAGCTTGGTAGGT- 3¢, en-compassed the ATG start codon of cyp and contained a 5¢ extension with an NdeI site. The reverse...

Báo cáo khoa học: Calcium-independent phospholipase A2-mediated formation of 1,2-diarachidonoyl-glycerophosphoinositol in monocytes potx

... short-lived species for the initial incorporation of AA into the PtdIns class of cellular phospholipids in human monocytes.AbbreviationsAA, arachidonic acid; BEL, bromoenol lactone; cPLA2, calcium-dependent ... arachidonic acid (AA) incorporatelarge quantities of this fatty acid into choline and ethanolamine glycero-phospholipids, and into phosphatidylinositol (PtdIns). Utilizing liquid chro-matography coupled ... Proc Natl Acad Sci USA 92,8527–8531.11 Balsinde J, Balboa MA & Dennis EA (1997) In amma-tory activation of arachidonic acid signaling in murineP388D1macrophages via sphingomyelin synthesis.J...

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

... of complex containing MT1-MMP and furin, and the expression of a catalyticallyinactive dominant negative furin construct affected the processing of pro-MT1-MMP. Taken together, thesedata reveal ... immunoprecipi-tated with the FLAG antibody and the associated MT1-MMP was detected by IB using the MYC tag antibody. Top black arrowheads indicateIgGs. The bottom black arrowhead indicates a crossreaction. ... P)1,P)2,etc.) [46]. Similarly, a reduced interaction was observedbetween GRASP55 and TGF-aD152 (lacking the lasteight amino acids of the ICD) compared to the TGF -a D158 construct lacking only the last...

Tài liệu Báo cáo khoa học: Hypoxia-inducible factor-1a blocks differentiation of malignant gliomas pdf

... reverse, 5¢- GACCTTCTTCTCCCGCATCATC- 3¢. VEGF: forward, 5¢- ACGAAAGCGCAAGAAATCCC- 3¢; and reverse, 5¢- TTAACTCAAGCTGCCTCGCC- 3¢. Beta-actin: forward, 5¢- AGGCTCTTTTCCAGCCTTCCT- 3¢; and reverse, 5¢- GTCTTTACGGATGTCAACGTCACA- 3¢. Xenograft ... discriminateaggregates.siRNA-mediated knockdown of HIF- 1a and VHLexpression The DNA sequence corresponding to the targeting siRNAsis: HIF- 1a: 5¢- TCGACAAGCTTAAGAAAGA- 3¢; VHL: 5¢- CCAAGACACCTCGAGAAT- 3¢ (Ribobio, CN). The scrambled ... dilution; Cell Signaling Technology), visualizationwas achieved with enhanced chemiluminescence (AmershamPharmacia Biotech, Piscataway, NJ, USA) using a Gene-Gnome chemiluminescence imaging and analysis...

Tài liệu Báo cáo khoa học: Calcium-independent phospholipase A2 mediates proliferation of human promonocytic U937 cells pptx

... as the cells entered G1 and then increasingagain as the cells approached and entered S phase. The same pattern of variation of iPLA2activity wasdetected whether the assay was conducted with ... iPLA2,asasn-2 lipase, may also participate in generating free fattyacids such as AA, which could subsequently be metab-olized to eicosanoids. The importance of AA and the eicosanoids as ... itis becoming increasingly clear that PLA2s differ withrespect to substrate speciﬁcity, co-factor requirementsfor activity, and cellular localization [1–5]. Mammaliancells usually contain several...

Tài liệu Báo cáo khoa học: Cytochrome P450 Cyp4x1 is a major P450 protein in mouse brain doc

... comparison of secondary struc-ture data ) from the crystal structure in the case of CYP102 – and predicted using jpred [38] in the case of CYP4x1. Coordinates for the main chain atoms of alignedCYP4x1 ... showing that Cyp4x1 is a major brain P450. Immunohistochemical localization of the Cyp4x1 protein in brain showed speci c staining of neurons, choroids epithelial cells and vascular endothelial cells. ... predicted CYP4x1 protein has characteristics of a functional P450, including conservation of the haem-binding cysteine in the RNCIG motif, and so weundertook molecular modelling to examine its...

Tài liệu Báo cáo khoa học: 1. Signal Transduction 1.1 Integration of Metabolism and Survival pdf

... rhythmicity of the principal circadianclock located within the suprachiasmatic nucleus (SCN) isentrained predominantly by a light–dark cycle through pathwaysemanating from retinal photoreceptors. Via ... field and their implications.1.5 Signaling and Cancer: Nuclear Receptor ConnectionS1.5-1Androgen action and prostate carcinogenesisF. Saatç iog˘luDivision of Cellular and Molecular Biology, ... revealed that FAK defici-ency leads to reduced incidence of papillomas and inhibition of progression to carcinomas. Mechanistically, loss of FAK is asso-ciated with increased apoptosis in both...

Tài liệu Báo cáo khoa học: Si-face stereospeciﬁcity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic Archaea as determined by mass spectrometry ppt

... withrespect to C5 of the synthetic deazaflavin and others to be Re-face stereospeci c [7,8]. In case of the pyridine-nucleotide-dependent enzymes the redoxpotential (E°) of the electron acceptor reduced ... Stereochemical studies of a selenium-containing hydrogenase from Methanococcusvannielii: determination of the absolute configuration of C- 5 chirally labeled dihydro-8-hydroxy-5-deazaflavincofactor. ... near neighbourhood of C5 that couldinteract with the reactant or the product, or affect the transition state(s) and by that induce an intrinsic ener-getic difference in the reaction profiles involving...

Tài liệu Báo cáo khoa học: "Multilingual Pseudo-Relevance Feedback: Performance Study of Assisting Languages" doc

... (defendending), martina, jovotna, navratilovaGERMAN '01: TOPIC 91ESAI in LateinamerikaLa gripe aviar en América LatinaAI in Latin America0.4560.098international, amnesty, strassenkind ... LinguisticsMultilingual Pseudo-Relevance Feedback: Performance Study of Assisting LanguagesManoj K. Chinnakotla Karthik Raman Pushpak BhattacharyyaDepartment of Computer Science and EngineeringIndian Institute ... MultiPRFapproach with French, German and Finnish assource languages and Dutch, English and Span-ish as the assisting language. We later vary the assisting language, for each source language and study...

Tài liệu Báo cáo khoa học: "Lexicographic Semirings for Exact Automata Encoding of Sequence Models" pdf

... LinguisticsLexicographic Semirings for Exact Automata Encoding of Sequence ModelsBrian Roark, Richard Sproat, and Izhak Shafran{roark,rws,zak}@cslu.ogi.eduAbstract In this paper we introduce a novel ... with a brief evaluation of the cost of intersection relative to failure transitions in comparable situations.2 The Lexicographic SemiringWeighted automata are automata in which the tran-sitions ... there is no n-gram arc labeled with w leaving the states, but there is a backoff arc; (3) there is no n-gram arc labeled with w and no backoff arc leaving the states; and (4) there is both an...

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