Báo cáo khoa học: "Last but Definitely not Least: On the Role of the Last Sentence in Automatic Polarity-Classification" pdf

... experiment we examined the readers’ rating of the same re-views based on reading single sentences ex-tracted from these reviews: the last sentence or the second one. The second sentence could ... frequently appear in final sentences. Thus, they first focused on the last sentence of posts as representing the polarity of the entire posts. Then, they divided the posi-tive category into 2 sub-categories ... Proceedings of the ACL 2010 Conference Short Papers, pages 331–335,Uppsala, Sweden, 11-16 July 2010.c2010 Association for Computational Linguistics Last but Definitely not Least: On the Role of...

Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc

... autoinhibited conformation, the SH2 domain binds to pYi on the C-terminal tail, and the Src homology 3 (SH3) domain binds to the linkerbetween the SH2 and kinase domains at the back of the small ... domain). Regardless of the slope, the active Src fractions increase during the Off to On transition, whereas the value of the inac-tive fraction (si) decreases if the slope is negative andincreases ... Bifurcation diagram in the plane of the rate constant k1andtotal Src abundance. k1is the rate constant of dephosphorylation of inhibitory tyrosine in the Src C-terminus. Types of bifurcationboundaries...

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... Ab(L1–40) is the dominantprotein in the inclusion bodies. In contrast, the majorprotein in the Ab(M1–42) inclusions was not Ab, but was the small heat shock protein IbpB (accession num-ber ... correspondence with the expected composition, indicating that the peptide is of the correct sequence and free of contaminating pro-teins. Five cycles of N-terminal sequencing conﬁrmed the expected ... with noindication of any product resulting from spontaneouscleavage of the N-terminal methionine in E. coli(Figs 4, S1 and S2). Co-expression of the E. coliaminopeptidase methionine aminopeptidase...

Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-speciﬁc alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt

... 1c contributed to the 5¢ nontranslated region of isoform 1 mRNA in which the common exon 2 contains an initiation codon.Intronic 5¢ consensus GT was located adjacent to the 3¢ end of each exon. ... around the transcriptional initi-ation sites.Determining the translation initiation site of isoform 2 KLK11Messenger RNA for isoform 2 contains two initiationcodons near the 5¢ end. To examine ... studied the translational regulation of KLK11 expression. Human KLK11 isoform 2 mRNAhas two translational initiation codons at the 5¢ por-tion of the open reading frame. The second initiationcodon...

Tài liệu Báo cáo khóa học: Active-site residues and amino acid speciﬁcity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

... 6); the G-A transition concurs with the use of hydroxylamine as the mutagenic agent [14]. Therefore, the dfp-1 mutation is within the AAVAD motif of the C-terminal PPC synthetase (CoaB) domain of ... second-half reaction and are part of the active-site of CoaB; these residues may be directlyinvolved in binding of one of the substrates, the amino acidcysteine (Fig. 3). However, it cannot be excluded ... for the second-half reaction are conserved in CoaB proteins. Residues of the N-terminal part of the Escherichia coliCoaB domain that are conserved in CoaB (Dfp) proteins from all kingdoms of...

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... (F340) increases, whereas the ﬂuorescence intensity at 380 nm (F380) decreases upon the binding of Ca2+. The change in the ratio of F340 ⁄ F380 determined the change in Ca2+concentrationinside ... CaM-bindingIQ domain, did not increase the basal or serotonin-induced ERK1 ⁄ 2 phosphorylation. The ERK1 ⁄ 2 phos-phorylation was inhibited in the presence of the calcium in ux inhibitor, CAI. The endogenous ... stimulation with serotonin, but is not direct evidence that Ras-GRF1 contributesto the serotonin-induced activation of Ras andERK1 ⁄ 2. Pretreatment with H89 eliminated the sero-tonin-induced...

Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx

... bFGF in the absence of FSH (data not shown).Direct implic ation o f cAMP increase, the second mes-senger of FSH, in the bFGF regulation was evaluated byaddition to the culture medium of either ... wasperformed using the SECURIAprogram from the PackardInstrument Company (Meriden, CT, USA).DNA quanti®cation The DNA content of the cell layer at the end of incubationwas quanti®ed by the method of ... extraction, cells were incubated for 24 heither in the absence or in prese nce of FSH, dbcAMP,cholera toxin or bFGF, either in combination with FSH ordbcAMP or cholera toxin and bFGF.Extraction of...

Báo cáo khoa học: Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis docx

... Microinjection of NLS-containing substrates in a myoblast. The movie represents the NLS microin-jection experiment shown in Fig. 6A.Video S2. Microinjection of NLS-containing substrates in a myotube. The ... represents the NLS microin-jection experiment shown in Fig. 6A.Video S3. Microinjection of NES-NLS-containing sub-strates in myoblasts. The movie represents the NLSmicroinjection experiment shown in ... function in protein export, rather than the increase of Nup358 alone, may cause the activation of CRM1 in myotubes. It is therefore likely that anincrease in Nup358 during myogenesis supports the CRM1...

Báo cáo khoa học: Relation between domain evolution, speciﬁcity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot

... (domain A) and the four domains B, C, D and E. In the case of four-domainproteins without an N-domain, only domain E (but not domain D) is present. It should be noted that the function of domain ... enzymescontaining such a domain. Throughout this paper, domainE is referred to as SBD, the starch-binding domain. In a classiﬁcation of carbohydrate-binding modules, thisstarch-binding domain is considered ... starch-binding domain (Eur. J. Biochem. 270) 645Relation between domain evolution, speciﬁcity, and taxonomy of the a-amylase family members containing a C-terminalstarch-binding domainSˇtefan...

Báo cáo khoa học: Transcriptome proﬁling analysis reveals multiple modulatory effects of Ginkgo biloba extract in the liver of rats on a high-fat diet pdf

... regulation, the blocked green lines indicate negativeregulation, and the dashed lines indicate indirect inhibition. The black lines indicate substance conversion or transportation ﬂow. The dashed lines ... GBE50 intake, althoughFoxa3 remained upregulated in group HG. In addi-tion, the decreased expression of Pc in group HG fur-ther suggested impaired glyconeogenesis. Upregulation of the insulin ... reticulumprotein that blocks the processing of sterol regulatoryelement-binding proteins (SREBPs) by binding toSREBP cleavage-activating protein, thus preventing the latter from escorting SREBPs to the...

Xem thêm