... an unusual PDE from this protozoal organism. This enzyme, TcrPDEC, is a member of the classI PDEs, as determined from the presence of a characteristic signaturesequence and from the conservation ... LmPDEC, phosphodiesterase from Leishmania major; PtdIns(3)P, phosphatidylinositol-3-phosphate; TbPDEC, phosphodiesterase from Trypanosoma brucei; TcrPDEC, phosphodiesterase from Trypanosoma cruzi.6412 ... purchased from Moravek Biochemi-cals Inc (Hartmann Analytik, Zurich, Switzerland). cAMPand cGMP (sodium salts) were obtained from Sigma(Buchs, Switzerland). PDE inhibitors were from the follow-ing...