Báo cáo khoa học: Role of conformational flexibility for enzymatic activity in NADH oxidase from Thermus thermophilus pptx
Báo cáo khoa học: Role of conformational flexibility for enzymatic activity in NADH oxidase from Thermus thermophilus pptx
... 4897 Role of conformational flexibility for enzymatic activity in NADH oxidase from Thermus thermophilus Gabriel Zˇolda´k1,Ro´bert Sˇut’a´k1,2, Maria´n Antalı´k1,3, Mathias Sprinzl4and ... the role of the homodimericstructure of NADH oxidase. Keywords: NADH oxidase; conformational dynamics; flavo-proteins; fluorescence quenching; Thermus thermophilus. The activity and stability of ... flexibility of the activesite. We have studied the effect of urea on NADH oxidase (EC 1.6.99.3) from Thermus thermophilus. NADHoxidaseis a dimeric flavoprotein containing one molecule of FMNin...
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Báo cáo khoa học: Role of Ca2+/calmodulin regulated signaling pathways in chemoattractant induced neutrophil effector functions Comparison with the role of phosphotidylinositol-3 kinase ppt
... switch of a5b1integrin in CHO cells,suggesting also a role for Ca2+/calmodulin-dependentmolecules in regulating the affinity state of integrins onneutrophils [48].Although PtdIns3K has ... calmodulin and PtdIns3K. In guinea pig neutrophils, it has been demonstrated thatinhibition of calmodulin results in inhibition of Rac and p21activated kinases [50]. Involvement of Rac in ROS ... can bind to downstream targets, such as theCa2+/calmodulin-dependent kinases (CaMKs) [21,22].CaMKs form a family of serine/threonine kinases activatedby binding of Ca2+/calmodulin and includes,...
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Báo cáo khoa học: Role of glutaredoxin 2 and cytosolic thioredoxins in cysteinyl-based redox modification of the 20S proteasome docx
... fragmentation using massspectrometry, by incubating Grx2 in standard buffer for 30 min or 2 h in the presence of n-PT. After incu-bation, standard Grx2 and fragments recovered by fil-tering the incubation ... novel monothiol glutaredoxins from Saccharomyces cerevisiae provide further insightinto iron–sulfur cluster binding, oligomerization, and enzymatic activity of glutaredoxins. Biochemistry 47,1452–1463.23 ... glutaredoxin plays acentral role in protection against protein oxidativedamage in Saccharomyces cerevisiae. Mol Cel Biol19,8180–8190.19 Gan ZR (1992) Cloning and sequencing of a geneencoding...
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Báo cáo khoa học: Role of the plasma membrane leaflets in drug uptake and multidrug resistance ppt
... active uptake of TMRM into the mitochon-dria interferes with the second phase of TMA-DPHquenching (Fig. 2). Initially, there is significant inhibi-tion of the quenching in the respiring cells, ... phase of fluorescence in the Pgp-over-expressing cells or fluores-cence quenching in wild-type cells (Fig. 5). Inhibition of Pgp with cyclosporine A caused a parallel increase in the amount of TMRM ... leaflet of the membrane, slowing down entryinto the cell; indeed, for an anthracycline such asdoxorubicin, this takes approximately 1 min, givingthe Pgp pump ample opportunity to deal with the in ux...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
... of R120 by lysine reduces the probability of forming the fully closed form of the protein.The results obtained with the K22V/R120K doublemutant protein point toward a central role of arginine ... enolpyr-uvyl-accepting substrate, shikimate-3-phosphate, does notinteract with R124 in the ternary complex [20]. Hence, themechanism of binding of fosfomycin by reinforcement of aninitial binding site (mainly ... chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin binding to the K22V and K22E mutant proteins.However, two other positively charged amino...
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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx
... mediator of lethal sepsis, triggering theproduction of proinflammatory cytokines and liverinjury [13].Therefore, the present study aimed to elucidate the role of KCs in the regulation of CYP isoform ... [22]. ROS indirectly reduced the activity of selected isoforms of CYP by inducing phosphorylation of the isoforms [23]. Activated KCs cause oxidativestress on the surrounding tissue, releasing largeamounts ... groups For depletion of KCs in vivo, 7.5 mgÆkg)1 of GdCl3wasinjected via the tail vein at 48 and 24 h before the perfor-mance of CLP or sham operation, based on the findings of other investigators...
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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx
... have indi-cated that the CagAPY-induced cell elongation pheno-type also involves tyrosine dephosphorylation of cortactin, vinculin and ezrin, which are three well-known actin-binding proteins ... phosphatasesinvolved in this scenario, however, remain unknownand do not require Shp2. Instead, CagAPYcan inhibitSrc activity both by direct interaction of both proteinsor by binding of CagAPYto ... distribution,making them unsuitable for the investigation of b-cate-nin signalling [29]. In support of this aspect, usingMadin–Darby canine kidney cells that expressE-cadherin and b-catenin without any mutations,...
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Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt
... survival resulting from activation of PPARb.Activation of PPARb by LD induces CERK mRNAexpression and increases CerK activity in SP1cellsTo investigate the role of CerK and its regulation of Cer ... culturedhuman keratinocytes [14]. In this study, we examined the interaction betweenCerK and PPARb, and its role in regulating keratino-cyte survival. We report that in a mouse keratinocytecell line upregulation ... role in skin woundhealing. After a skin injury, PPARb expression is rap-idly elevated in the epidermis at the wound edges; dele-tion of a single PPARb allele results in delayed woundhealing...
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Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidification and cathepsin D in cholera toxin cytotoxicity pdf
... prepared from CT and CT-B-injected rats (Fig. 3A). An increase in ARF content was observed in endosomal fractionsisolated 5 min postinjection of CT-B, and this increasewas maintained for up to 60 min ... action of cholera toxin in rat liver parenchyma. Following administration of a saturating dose of cholera toxin to rats, rapid endocytosis of both cholera toxin subunits wasobserved, coincident ... and0.5% saponine in NaCl ⁄ Pi for 10 min. Permeabilized cellswere blocked for 20 min with 10% horse serum in NaCl ⁄ Pi,and then incubated for 60 min with a rabbit polyclonalanti-dinitrophenyl...
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Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc
... vector by using Lipofectamine (Invitrogen).Cells were selected in neomycin, and clones were isolatedand screened for Bcl-XLexpression.Creation of TAM67-overexpressing cell lineThe dominant-negative ... transcription, were increased in response toEGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinasekinase, inhibited the EGF-induced c-Fos and c-Jun expression, AP1 DNAbinding, Bcl-XLexpression, ... at various time points for the immunoblotanalysis of caspase 9. Beginning at 6 h post treatmentwith ADR, an increase in the amount of the cleavedform of caspase 9 was seen in ADR-treated cells(Fig....
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