Báo cáo khoa học: Inhibition of a-synuclein fibrillization by dopamine analogs via reaction with the amino groups of a-synuclein Implication for dopaminergic neurodegeneration pot
... (2005) 3661–3672 ª 2005 FEBS
Inhibition of a-synuclein fibrillization by dopamine analogs
via reaction with the amino groups of a-synuclein
Implication for dopaminergic neurodegeneration
Hong-Tao ... incubated with 1 mM of DA analogs for 24 h, and then the reaction mixtures were analyzed by means of
SEC. (B) SDS ⁄ PAGE graph of the fr...
... require
interaction with, nor methylation, of the PP2A C subunit
[17].
Little is known about the molecular basis for the
interaction of the B subunits with the AC heterodimer.
None of the B subunits ... sequence
similarities between the families, they do have signi®cant
sequence homology within each family. For example, within
the B56 family, each isoform shares a...
... pre-incubated for 5 min, the reaction was
initiated by the addition of 500 lm (dl)-homocysteine and
incubated at 37 °C for a further 30 min, unless otherwise
stated. The reaction was terminated by the ... shows the superimposed structures of
DHPS and 5-methyltetrahydrofolate. The model of the
methyltetrahydrofolate-binding site of MetS with one
molecule of...
... FEBS 369
Thermal unfolding transition in the absence
and in the presence of the different ligands
The influence of ligand binding on the thermal stability
of C-LytA was studied by monitoring the CD ... but also at the active site [18]. There-
fore, inhibition of the cell wall lytic activity of Pce by
atropine and ipratropium could take place by interfer-
ence...
... 2B). The addition of
20 l
M
Zn-ferrocyanide in the reaction medium from the
beginning of the measurement leads to the appearance of
the reduced form of the enzyme, even in the presence of
the ... relative to the
free protein c onformations [26,50]. The protein–protein
interaction also affects the microenvironments of the two
prosthetic groups. In the...
... Fe
2+
–EDTA. The presence of this
metal-ion complex enhanced the inhibition observed with
these enzymes consistent with the o ccurrence of radical-
mediated reactions. Overall, these studies ... removal of H
2
O
2
before the
peroxides were u sed in other experiments. Omission of the
rose bengal, photolysis in the absence of O
2
, or incubation
of complete samples...
... immunoreactivity (IR). The staining was semi-
quantitatively evaluated by assigning a score for the inten-
sity of the IR and for the proportion of cells positively
stained. The intensity of IR (intensity ... cells. Therefore,
the promoter sequence of MDR-1 ()136 to 10) was
amplified, and the luciferase reporter assay was
performed to investigate the transcription...
... hydrolysis of
[
32
P]ATP[cP] in the presence of 0–400 ng of IRE
(Fig. 8). Compared to the amount of ATP hydrolyzed
with 400 ng of IRP alone, addition of IRE increased
the release of inorganic ... complex formation. Further-
more, ATP-cS, but not AMP-PNP, inhibited IRP–IRE
binding in extracts from fibroblasts treated with the
analogs in culture [41]. Therefore, the...
... only by an excess of the same, un-
labeled peptide, but also by an excess of the unlabeled
peptide from the other binding sites (data not shown).
The validity of the results obtained for the binding
of ... channels are formed by 4 of such subunits involving the C-terminal assembly
domain. (C) Magnified sections of the peptide array: The numbers indicate t...
... generated in the
intestinal scrapings by a-amylase present in the hetero-
geneous sample or by activity in the C-terminal
domain of the full-size protein, thereby accounting for
the inhibition by ‘acarbose’ ... 80% of the maltase
activity, while MGA accounts for all glucoamylase
activity, 20% of the maltase activity, and 1% of the
sucrase activity [10]. Tog...