Báo cáo khoa học: Catalytic residues Lys197 and Arg199 ofBacillus subtilis phosphoribosyl diphosphate synthase Alanine-scanning mutagenesis of the flexible catalytic loop ppt

... Catalytic residues Lys197 and Arg199 of Bacillus subtilis phosphoribosyl diphosphate synthase Alanine-scanning mutagenesis of the flexible catalytic loop Bjarne Hove-Jensen, ... interaction of hydrophobic aminoacids of b-strands located on either side of the flexible catalytic loop. The tightly packed interface prevents the closure of the loop, and thus, prevents the interaction of ... topologically and functionally equivalent to a loop, variously designated the flexible loop, the catalytic loop, or loop II of the class I phosphoribosyltransferases. This loop isinvolved in the catalytic...

Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx

... but the differentiation between these two types of enzymeis not clear [4]. Looking at the sequences of the twoenzymes, both show absolute conservation of the histi-dine residues of the CuA and ... determined at pH 5 and 0.05% SDS, and DO activity at pH 7 and 0.02% SDS.Fig. 3. Dependence of the TH activity of extracts of R3 and the two mutant strains on the presence of cofactor,L-dopa. ... be part of the defense sys-tem against wounding and infection by the pathogen[16]. Thus, the timing and location of melanizationseems to be essential for the prevalence of one of thesetwo...

Báo cáo khoa học: Human-blind probes and primers for dengue virus identiﬁcation Exhaustive analysis of subsequences present in the human and 83 dengue genome sequences doc

... of south-east Asia, parts of Africa, and Southern and Central America. The Aedes aegypti mosquito, whichprefers to feed on humans, is a carrier of the denguevirus and is commonly found on the ... tohybridize with the probe sequences. Figure 5 shows the overlapping expression patterns for two pairs of genomes for the set of 216 probes. The distribution of the number of probes present on the 216-probe ... away from the nearest human sequence (Fig. 2). The results of whichare provided in the Supplementary data. The presenceor absence of each n-mer was calculated, rather than the frequency of occurrence....

Tài liệu Báo cáo khoa học: An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori docx

... achieved.Determination of the structure of 8K-BLPMALDI-TOF MS analysis of the purified materialfrom the final HPLC yielded a monoisotopic mass of 7409.2 ([M + H]+) (Fig. 3A). Amino acid sequenceanalysis of the ... instead of as aheterodimer. Second, 8K-BLP was predominantly pro-duced by the fat body, a functional equivalent of the liver and adipose tissue of vertebrates, and the liver is the major source of ... alsoaffect the development of the wing disks at the prepu-pal stages. It is interesting to note that bombyxin-IIalso stimulated the growth of the wing disks in the butterfly Precis coenia and the hawkmoth...

Tài liệu Báo cáo khoa học: "Discovering Global Patterns in Linguistic Networks through Spectral Analysis: A Case Study of the Consonant Inventories" pdf

... example, the prin-cipal eigenvector (i.e., the eigenvector correspond-ing to the largest eigenvalue) is the direction inwhich the sum of the square of the projections of the row vectors of the ... we are aware of that analyzes the eigenvectors to obtain a two dimensional visualize of the network. Nevertheless, the work does notstudy the spectrum of the graph. The aim of the present work ... threetaken together. The individual contribution of the other eigenvalues to the spectrum is signiﬁcantlylower than that of the top three. Third, the eigen-values on either ends of the spectrum...

Báo cáo khoa học: Cysteine residues exposed on protein surfaces are the dominant intramitochondrial thiol and may protect against oxidative damage docx

... some of these potentially protec-tive reactions can be made. The reaction of •NO2with the thiols of GSH or cysteine is fast (  3–5 · 107m)1Æs)1) [29] and the rate of reaction of ONOO)with ... occurbecause of the rapid reaction of thiols with many of the damaging species present in biological systems.Furthermore, because many of these potentially pro-tective thiol reactions occur through the ... part of a general antioxidantcycle within the mitochondrial matrix, the location of the exposed thiols on the surface of proteins may alsoprevent oxidative damage to the proteins on whichthey...

Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf

... mutation of three of these basic residues within the N-domain of ClpA (H94, R86 and R100) did not alter substrate degradation. In contrastmutation of two conserved arginine residues (R90 and R131), ... initiatedthrough productive binding to the substrate via twoelements in ClpA, one in the N-domain and the otherin the pore of the ClpA hexamer. In the case of shortunstructured peptides or unfolded ... utilizes two structural ele-ments, one in the N-domain and the other in the pore of the hexamer, both of which are required for efﬁcient unfolding of some protein substrates.AbbreviationsAAA+,...

Báo cáo khoa học: Contributions to catalysis and potential interactions of the three catalytic domains in a contiguous trimeric creatine kinase doc

... CKdomains. It is not known whether the active sites in each of the contiguousﬂagellar domains are catalytically competent, and, if so, whether they arecapable of acting independently. Here ... MgADP, creatine and nitrate are bound to CKs. This transition involves the movement of two ﬂexible loops (residues 60–72 and 323–333 in both Torpedo and rabbit CyCKs) and at the N-terminus, over ... the dimer [15]. Incontrast, the recently published crystal structure of the TSAC of rabbit muscle CK [6] (and the TSAC struc-ture for Torpedo [5]) is highly asymmetrical, with onlyone of the...

Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt

... identify the activesite residues involved in the binding of Glc to the super-open state (Fig. 1C) of this two domain [large(L) and small (S)] enzyme, and thus the site of initia-tion of the global ... in the L-domain (residues N204, D205, N231, E256 and E290) and the S-domain (residues T168 and K169) (Fig. 1B). To identify the contact residues involved in the initial binding of Glcto the ... [27–29].Two of these (Y214C and Y215A) are localized inhelix 6 and three (V455M, A456V and A460R) inhelix 17, and some of these missense mutations (e.g.Y215A and V455M) perturb the hydrophobic...

Báo cáo khoa học: Expression, puriﬁcation and catalytic activity of Lupinus luteus asparagine b-amidohydrolase and its Escherichia coli homolog potx

... ccording to Parry and Clark [37], expression of the ybiK gene responded to the presence of cysteine and todefects in t he cysBgene,andtheybiK knockou t mutationimpaired the use of glutathione ... 30 m in and the pellet was removed. To the supernatant, further PEG8K was added to a concen-tration of 35% and stirring continued for 1 h . The solutionwas then centrifuged as before. The supernatant ... resolution of the trees was too low forseparation of some branches. One of the branches comprisesmainly archaeal enzymes, another one contains eukaryotic and bacterial aspartylglucosaminidases, and the...

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