... 75 mM; lane 3, 100 mM; lane 4, 125 mM; lane 5, 150 mM;lane 6, 200 mM; lane 7, 250 mM; lane 8, 300 mM; lane 9, 500 mMNaCl. HS and LS, high and low molecular mass standards with themolecular ... spectrometry, amino acid analysis andsequencingAmino acid analysis was performed at the Amino AcidAnalysis Center, University of Uppsala, Sweden. Sequenceanalysis was performed using an Applied BiosystemsProcise ... USA), and purified using reverse-phase HPLC. For both synthetic and recombinant Ab peptides, thecorrect mass was confirmed by MALDI-TOF MS andLC-MS.PCR and cloning procedureSynthetic genes for...