Báo cáo khoa học: Substrate positioning by His92 is important in catalysis by purple acid phosphatase docx
Báo cáo khoa học: Substrate positioning by His92 is important in catalysis by purple acid phosphatase docx
... 2977 Substrate positioning by His92 is important in catalysis by purple acid phosphatase Enrico G. Funhoff1,4, Yunling Wang2, Goran Andersson2and Bruce A. Averill1,31 Swammerdam Institute ... imidazole group of His92. The large (15- to 30-fold) increase in KMobservedfor His92Ala-recRPAP suggests that this residue is involved in substrate binding in PAPs. In principle,however, an ... role of His92 in the catalytic mechanism is unknown. Site-directed mutagenesis of His92 was performed to examine the role of this residue in single polypep-tide PAP. Conversion of His92 into Ala,...
- 10
- 332
- 0
Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt
... I (NF-I) required to facilitateinitiation of replication in vivo but not in vitro, perhaps by alleviating nucleosome repression or by assisting in originunwinding under conditions of altered ... findings that JCV DNA replication is stimulated by NF-I in vivo but not in vitro support thisexplanation [50]. This view is consistent with the knowledgethat introducing an SV40 sequence into ... in the synthesis of products greater than fournucleotides in length. Murine DNA polymerase a-primase is absolutely inactive in initiating SV40 DNA replication in an assay consisting of purified...
- 8
- 326
- 0
Báo cáo khoa học: Suppression of nuclear factor-jB activity in macrophages by chylomicron remnants: modulation by the fatty acid composition of the particles pot
... forimmunoblotting which were quantified using the bicinchoni-nic acid protein assay.Statistical analysisData were analysed by one-way ANOVA followed by Tukey’s test (single time point) or two-way ... Libby P (2007) In ammatory mechanisms: themolecular basis of in ammation and disease. Nutr Rev65, S140–S146.2 Libby P (2002) In ammation in atherosclerosis. Nature420, 868–874.3 Albertini ... for-mation is in uenced by dietary fatty acids during theirtransport from the gut to the liver in CMR in thepostprandial phase. In this study, we investigated the effects of CMR onNF-jB activation in...
- 14
- 328
- 0
Tài liệu Báo cáo khoa học: "The Impact of Query Refinement in the Web People Search Task" docx
... datasets forthis clustering task, with over 15 research groupssubmitting results in each campaign. Since the re-lease of the first datasets, this task is becoming anincreasingly popular research ... search(WePS) a challenging task, because every singlename is usually shared by many different peo-ple. One of the mainstream approaches to solvethis problem is designing meta-search engines thatcluster ... befiltered by the user by adding query terms.Our results indicate that, although in mostoccasions there is a query refinement thatgives all and only those pages related toan individual, it is unlikely...
- 4
- 459
- 0
Báo cáo khoa học: Membrane binding of SRP pathway components in the halophilic archaea Haloferax volcanii docx
... domains contain fewer arginine and lysineresidues than A domains of other archaeal FtsY proteins. The amino acid composition of the first 46 positions of archaeal FtsY proteins areshown. Arginine ... contains 14 positively chargedresidues within the first 46 positions, while Pyrococcusfuriosus FtsY contains 12 arginine and lysine residues in thisportion of the protein. In contrast, examination ... domain II anddomain III regions of the eukaryal SRP19 protein [17]. In further contrast to the situation in Eukarya, where SRP19binding to SRP RNA is a necessary prerequisite for SRP54binding,...
- 9
- 468
- 0
Báo cáo khoa học: Protein kinase Ch activity is involved in the 2,3,7,8tetrachlorodibenzo-p-dioxin-induced signal transduction pathway leading to apoptosis in L-MAT, a human lymphoblastic T-cell line potx
... protein wasexamined in an in vitro nPKC kinase assay, using as the substrate biotin-PKCh pseudosubstrate peptide, which is a rather selective substrate for PKCh and PKCd.PKCh in TCDD-induced ... apoptosis is induced by TCDD, and the failure of actinomycin D(an inhibitor of gene transcription) and cycloheximide(an inhibitor of de novo protein synthesis) to block thisapoptosis, led ... transductionmechanism involved in L-MAT cell apoptosis.ResultsInhibition of TCDD-induced L-MAT cell apoptosis by rottlerin, an nPKC inhibitor12-O-Tetradecanoyl phorbol-13-acetate (TPA) was used in combination...
- 13
- 426
- 0
Báo cáo khoa học: Myocyte enhancer factor 2B is involved in the inducible expression of NOX1⁄ NADPH oxidase, a vascular superoxide-producing enzyme ppt
... )125 in the 5¢-flanking region containing aconsensus binding site for myocyte enhancer factor 2 (MEF2), to whichbinding of MEF2 was augmented by PGF2a. Gene silencing of MEF2B by RNA interference ... expression of MEF2B in VSMC is governed by ATF-1 itself.DiscussionThe major lines of evidence provided by this study areas follows: (a) the 5¢-terminus of the rat NOX1 mRNAexpressed in VSMC was a ... NADPHoxidase, NOX5, is expressed in human VSMC [14],but not in rodents.The MEF2-binding site in the promoter region wasshown to be elemental for induction of NOX1 expres-sion in VSMC. This site was...
- 9
- 452
- 0
Báo cáo khoa học: Voltage- and Ca2+-activated potassium channels in Ca2+ store control Ca2+ release docx
... 7.3 by add-ing KOH, and 10 lm ionomycin.Electroporation of retinal cells for introducingiberiotoxinAn E3 retina was positioned between a pair of platinumelectrodes separated by 0.6 mm in ... E3 chick retina with the DiOC5(3)staining [31]. In the present study, we studied the chan-ges in the DiOC5(3) fluorescence intensity in the E3chick retina to gain an insight into the dynamics ... DiOC5(3)-stained retina. Itcaused an increase in the DiOC5(3) fluorescence(Fig. 2B), indicating that a negative shift is caused in the luminal potential. The negative shift may be due toa decrease in...
- 13
- 340
- 0
Báo cáo khoa học: SLC39A14, a LZT protein, is induced in adipogenesis and transports zinc pptx
... [15]. Histidine-rich repeats areconsidered to be potential metal-binding domains[17,20,21]. Although another zinc transporting domain,HEXPHE, has also been found, the first histidine in the ... 66th amino acid in XM046677 (39th in D31887). The result of 5¢-RACEindicated that cDNA no longer existed in front of thefirst methionine of mouse SLC39A14. Additionally, in the amino acid sequences ... differentiation is definitely needed.Zinc is an essential metal in all eukaryotes. Zinctransporting proteins were first reported in yeast andplants. In mammals, the ZIP superfamily is the moststudied zinc...
- 10
- 323
- 0
Báo cáo khoa học: Substrate recognition and ®delity of strand joining by an archaeal DNA ligase docx
... LigTk.The ability to discriminate mismatched ends has beeninvestigated for DNA ligases from several organisms usingsynthetic duplex DNA substrates containing 3¢ or 5¢ mis-matches at their nicks ... single-strand break in double-stranded DNA [5,6]. They areessential enzymes for maintaining the integrity of thegenome during DNA replication [7], DNA excision rep air[8] a nd DNA recombination ... forelectrophoresis. The gel was stained with ethidium b romide. In experiments determining the kinetic parameters of LigTk,ligation reaction mixtures (20 lL) contained 20 mMBicine/KOH pH 8.0,...
- 7
- 251
- 0
Từ khóa: chuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIMÔN TRUYỀN THÔNG MARKETING TÍCH HỢP