Báo cáo khoa học: Quantitative assessment of the glyoxalase pathway in Leishmania infantum as a therapeutic target by modelling and computer simulation pot

... FEBS Quantitative assessment of the glyoxalase pathway in Leishmania infantum as a therapeutic target by modelling and computer simulation Marta Sousa Silva1, Anto´nio E. N. Ferreira1, Ana ... evaluating the relevance of the glyoxalase pathway as a potential therapeutic target by revealing the importance of critical parameters of this pathway in Leishma-nia infantum. A sensitivity analysis ... play only a minor role in maintaining a low intracellularmethylglyoxal concentration. The importance of the glyoxalase pathway as a therapeutic target is very small, compared to the much greater...

Báo cáo khoa học: Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure pdf

... remaining attached to the Asn. The effect of deglycosylation was analysed by SDS ⁄ PAGE and activity measurements. A single band at 90 kDa wasdetected in the deglycosylated laccase samples, in con-trast ... to affect the folding of the protein during the post-translational processing, and thereby the function of the mature laccase.Our structural analysis of wild-type MaL indicatedthat the last ... substrate, and the rate of substrate oxidation was measured by monitoring the change in absorbance over 5 min, using a Varioskan kinetic platereader (Thermo Electron Corporation, Waltham, MA,USA)....

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... reducible plastoqui-nones, on the quantitative parameters are discussed in terms of the origin of each kinetic phase.Abbreviations A O–J, A J–I and A I–P, amplitude of O–J, J–I and I–P phases, ... is accounted for by the O–J phase. Interestingly,this decrease of A J–Iwas compensated for by the equivalent increase of A O–J, making the sum of contri-butions from A O–J and A J–Iequal ... lowered and the subsequent rise was retarded.Kinetic information on each phase can be of greathelp in investigating the bioenergetics of the FI rise. In fact, the rate constants calculated for each...

Báo cáo khoa học: "Quantitative modeling of the neural representation of adjective-noun phrases to account for fMRI activation" doc

... which of the 24 exemplars the par-ticipant was viewing and thinking about. Sepa-rate classifiers were also trained for classifying the isolated nouns, the phrases, and the 4 seman-tic categories. ... and linear trend, and were temporally smoothed with a high-pass filter using a 190s cutoff. The data were normalized to the MNI template brain image using a 12-parameter affine transformation ... repetitions was left out for each fold. Linear regression assumes a linear dependency among the variables and compares the variance due to the independent variables against the vari-ance due to the...

Báo cáo khoa học: Identiﬁcation of the amniotic ﬂuid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms docx

... FEBS a mechanism for increasing the bioavailability of IGFs by enhancing the proteolytic cleavage as well as forproducing the C-terminal domain.We have examined the amino acid sequences of the other ... trophoblast and fetal–placental physiology and pathology and its level in maternal as well as in fetal circulation and in the liverincreases under hypoxic conditions in the uterus, result-ing in intrauterine ... chro-matographic proﬁles, as well as the peak area and shape, did not differ in a signiﬁcant manner (Fig. 7). The only major discrepancy was the presence of a peak, indicated as 1, in the trace of...

Báo cáo khoa học: Enzymatic features of the glucose metabolism in tumor cells ppt

... Maeda N,Nagashima Y, Tanaka T, Yamamoto S, Oka M &Nakamura K (2009) Detection of autoantibodiesagainst cyclophilin A and triosephosphate isomerase in sera from breast cancer patients by proteomic ... of BGP has beenproposed to be a potential early biomarker for humancolorectal carcinomas [204]. By contrast, in braintumor tissues (astrocytoma and glioblastoma) the activity of GP was found ... of enzyme-regulatory mechanisms operating below the control of gene expression was dramatically underesti-mated in the past decade, which was characterized by the innovation and broad application of high-through-put...

Báo cáo khoa học: Mutational analysis of the C-domain in nonribosomal peptide synthesis pot

... GAA TAC ATC GTA ggc CTC TAC CAG CAC ATG CAG-3¢ AatI5¢R6 7A 5¢-GGT AGA GAG ATA CGA TGT ATT Cgc gAC GTT GTT TAT CTA TGAAAA GC-3¢NruI3¢R6 7A 5¢-GCT TTT CAT AGA GAA ACA ACG Tcg cGA ATA CAT CGT ATC ... normalized the valuesobtained in all the assays for dipeptide formation a nd forDKP production by multiplying the counts in the radioac-tive assays and the area in the HPLC-assays by the ratio ... 5¢-CCA TGA GGA Tcc GAT GAA AGC TCC A- 3¢ BamHIMP(Y166W) 5¢-GCA AGG ACA Acc AtA TGG CAA GCA AGT C-3¢ NdeIMP(Y166F) 5¢-GCA AGG ACA Aga AgA TGG CAA GCA AGT C-3¢QuickChangeTM3¢Q1 9A 5¢-GCG TTG ACC...

Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx

... distinguished as monophyletic, the one of crayﬁsh(Astacidea) trypsins and a group that includes trypsinsfrom P. argus (Palinura), Brachyura, Penaeoidea, Cari-dea and Euphausiacea (Fig. 5).Although ... reliability. Backbone conformation was evaluated by the inspection of the Psi ⁄ Phi Ramachandran plot obtainedfrom procheck analysis [36]. Packing quality of the 3Dmodel was investigated by the ... (Qiagen, Hilden,Germany). RNA quality was assessed using the AgilentRNA 6000 Nano Assay Kit on an Agilent 2100 Bioanalyzer(Agilent Technologies, Santa Clara, CA, USA) and onlynondegraded samples...

Báo cáo khoa học: Functional role of the linker region in puriﬁed human P-glycoprotein pot

... as Arg680 and Leu682,respectively [22]. Trypsin cleavage increased the basal,verapamil-stimulated and vinblastine-stimulatedATPase activities, suggesting that cleavage of the linker activates ... increased ATPase activityalso indicates that the linker region has another role as a suppressor of ATPase activity in the native P-gp. The ksub⁄ kbasalvalues of native P-gp were higher and ranged ... proteases at 20 °C for 1.5 h. The cleavage was stopped and ATPase activity was measured as indicated in the Materials and methods. All data represent the means ± SD from three independentassays.Transport...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... toconfirm the tetrameric association in solution. In the Mn-bound form, the metal was in trigonal bipyramidal coordination with five ligands: four sidechain atoms and a water oxygen. One aspartate and ... 100°C.Int J Syst Bacteriol 46, 1070–1077.10 Kawarabayasi Y, Hino Y, Horikawa H, Yamazaki S,Haikawa Y, Jin-no K, Takahashi M, Sekine M, Baba S,Ankai A et al. (1999) Complete genome sequence of anaerobic ... sidechains and apical water coordinating the metal centerhad the same configurations as those in the metal-bound ApeSOD. This implies that the conformationaround the active site of ApeSOD is independent...

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