Báo cáo khoa học: Protein kinase Ch activity is involved in the 2,3,7,8tetrachlorodibenzo-p-dioxin-induced signal transduction pathway leading to apoptosis in L-MAT, a human lymphoblastic T-cell line potx

... molecular mechanism involved in the immunotoxicity of TCDD. In summary, we suggest that PKCh kinase activity is functionally involved in the TCDD-induced signal transduction mechanism leading to ... can be explained by the singleAhR pathway. The molecular mechanism involved in the AhR-independent pathway( s) leading to TCDD-induced immunotoxicity is not clearly understood, andindeed the ... PKCh and speciﬁcally sup-press its kinase activity. To conﬁrm that PKCh kinase activity really does participate in the signal transduc-tion mechanism involved in TCDD-induced L-MATcell apoptosis, ...

Báo cáo khoa học: Myocyte enhancer factor 2B is involved in the inducible expression of NOX1⁄ NADPH oxidase, a vascular superoxide-producing enzyme ppt

... E, Nishimatsu H, Satonaka H, Walsh K, Goto A, Omata M, Fujita T, Nagai R & Hirata Y (2002)Angiotensin II induces myocyte enhancer factor 2- andcalcineurin ⁄ nuclear factor of activated T ... NOX1, a catalyticsubunit of NADPH oxidase: involvement of mitochon-drial respiratory chain. Biochem J 386, 255–261.9 Fan C, Katsuyama M, Nishinaka T & Yabe-Nishimura C(2005) Transactivation ... understandingof regulatory mechanisms in vascular superoxideproduction.Experimental proceduresMaterialsPGF 2a was purchased from Nacalai Tesque (Kyoto, Japan).Antibodies against MEF2 and ATF-1...

Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf

... lgÆmL)1each aprotinin, leupeptin and pepstatin, twice in kinase buffer, and used for in vitro kinase assays. In vitro kinase assaysRecombinant human protein kinase CK2 holoenzyme(0.5 U, corresponding ... [11].Keywordsatypical protein kinase; casein kinase 2substrate; cell-cycle phase-dependentdegradation; protein protein interactions;Rio1 protein kinase 21CorrespondenceM. AngermayrE-mail: M.Angermayr@lrz.uni-muenchen.dePresent ... Bandyopadhyay A & Maitra U (2003) Casein kinase II phosphorylates translation initiation factor 5(eIF5). Saccharomyces cerevisiae. Yeast 20 , 97–108.28 Bandhakavi S, McCann R, Hanna DE &...

Báo cáo khoa học: Protein kinase Ce: function in neurons pot

... FEBSgene family, member A (RhoA) and led by inhibitionof the RhoA effector Rho-associated coiled-coil-containing protein kinase (ROCK), indicating thatattenuation of the RhoA-ROCK pathway is involved in ... whichare allosteric activators of the GABA A receptor[25,26]. GABA A receptors are ligand-gated Cl–chan-nels that are considered to be an important target ofethanol. GABA A receptors are ... essential for the sensation of thermal and in ammatory pain [38] and pro -in ammatory signals,including ATP and bradykinin, enhance TRPV1 activ-ity in a PKC-dependent manner [39,40]. Among the PKC...

Báo cáo khoa học: Protein kinase Ce: the mitochondria-mediated signaling pathway pot

... cellular signaling pathways. Apoptosis is a well-known pathway in which mitochondria are involved. Protein kinase Ce has beenclassiﬁed as a novel type of protein kinase C and is involved in manycellular ... in the apoptosis pathway comprises the intrinsic pathway, which is triggered by DNA damage.DNA-dependent protein kinase (DNA-PK), which is fundamentally involved in this pathway, is a nuclearserine ... related to apoptosis and translocation are shown.bProteins involved in mitochondria-mediated apoptosis. cProteins involved in mitochondrial import.PKCe and mitochondria H. Yonekawa and Y. Akita4008...

Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx

... glycation, rather thanpurely the loss of the parent amino acid, which is the key factor in terms of receptor recognition [29]. Thereare no data available on the extent of Lys (and otheramino acid) ... sufﬁ-cient to induce lipid loading in primary human macrophages, primarily via the scavenger receptorsSR -A and CD36. The accumulation of lipid in thesemacrophages is accompanied by increased endocytosisand ... myeloperoxidase to convert alpha-amino acids to a battery ofreactive aldehydes: a pathway for aldehyde generation atsites of in ammation. Biochemistry 37, 6864–6873.57 Proctor SD, Pabla CK & Mamo...

Báo cáo khoa học: Identiﬁcation of tyrosine-phosphorylation sites in the nuclear membrane protein emerin pot

... reticulum and folds at the nuclear pores into the INM, which is ﬁrmly attached to the lamina by integral membrane proteins of the INM. The INM proteins form complexes, transientlyor stably, with lamins, ... EDMD is characterized by a mislocalization ofemerin that is caused by the loss of emerin binding to lamin A (X- linked form) or by the loss of lamin A (autosomal dominant form) [22,23].Several ... lamins, chromatin proteins and a vari-ety of regulatory proteins, including transcriptionalregulators and splicing factors [1,2]. Attempts havebeen made to identify and catalogue the complete...

Báo cáo khoa học: Protein engineering of pyruvate carboxylase Investigation on the function of acetyl-CoA and the quaternary structure doc

... thisuncertainty and in fact such an undertaking is under way in this laboratory. Additionally, a protein engineeringapproach would be useful to examine the two partialreactions individually. In this study, ... The simple Michaelis–Menten equation wasused for the analysis of the data for bicarbonate andpyruvate.ATP cleavage assaysATP cleavage activity of intact PC and PC-(BC) wasassayed according ... domain which mediates the ﬁrst partialreaction and a carboxyl transferase (CT) domain whichcatalyzes the second partial reaction. The BC domain is located in the amino terminus of the single...

Báo cáo khoa học: Protein oligomerization induced by oleic acid at the solid–liquid interface – equine lysozyme cytotoxic complexes potx

... stable than human a- lactal-bumin in the presence of calcium towards thermaldenaturation and exhibited the same stability as human a- lactalbumin towards urea denaturation [56].This indicates ... otherstates of monomeric a- lactalbumin characterized to date. The same authors have shown that monomeric a- lactalbumin in the absence of fatty acids can bind to histone H3, which is the primary ... ﬁbroblast culture, a neuro-blastoma cell line (SH-SY5Y) and a rat phreochromcy-toma (PC12) cell line. Combined staining with acridineorange and ethidium bromide indicated that ELOAinduces apoptotic-type...

Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc

... response to twodifferent signal transduction pathways, the phorbolester-activated protein kinase C pathway, or the cAMP-dependent protein kinase A pathway [28]. Here, cAMPmay modulate AP-2 activity ... different signal- transduction pathways: protein kinase C and cAMP.Cell 51, 251–260.29 Garcia MA, Campillos M, Marina A, Valdivieso F &Vazquez J (1999) Transcription factor AP-2 activity is modulated ... of AP- 2a in thesetissues to upstream signaling pathways. They assessed the organization of a cis-regulatory region within the ﬁfth intron speciﬁc for directing AP- 2a expression to the developing...

Xem thêm