... 2004)
doi:10.1111/j.1432-1033.2004.04402.x
The interaction of the homeodomain of the sunflower KNOX protein
HAKN1 with DNA was studied by site-directed mutagenesis, hydroxyl
radical footprinting and missing nucleoside ... 380–413.
KNOX homeodomain DNA interactions M. F. Tioni et al.
202 FEBS Journal 272 (2005) 190–202 ª 2004 FEBS
Site-directed mutagenesis...
... isolated
and its genomic structure and expression analysis investi-
gated. This work will pave the way for further investigation
of the biological function of this gene, and the probability of
the ... products of b-actin. The x-axis indicates the time-periods of LPS incubation and the relative expression of the
carp IL-10 gene on the y-axis. Controls fo...
... i.e. if X agrees with
A and B agrees with X within a limited segment of
speech, then agreement between B and A is con-
firmed in 22.5% of the cases, while the probabil-
ity of the agreement class ... as:
is the vector of observations and
each is the index of a spurt. The probability dis-
tribution associated with each state of
the Markov chain only depends...
... language and means
of realization of its phenomena in a number of spe-
cific languages and is, thus, a kind of a "linguist in
the box" that helps non-professional acquirers with
the ... values and real-
ization options. Data about each parameter
includes the language, the name of the parameter,
the list of entities to which this parameter applies...
... for FHT [9], and the protein
concentration was adjusted to 0.371 mgÆmL
)1
in the sample.
The documented spectra show the accumulation of 10 scans
with 50 nmÆmin
)1
. The CD spectra of the FLS sample ... 4). These
enzymes probably evolved from a common ancestral gene,
and the essence of most of the conserved amino acids has
been further substantiated by site-di...
... activation.
On the other hand, further deletion of Fas up to part
of the DD (FD7 and FD2) or the lpr complementing
generalized lymphoproliferative disease (lpr
cg
)-type
point mutation (Val238 to Asn) in the ... nonapoptotic response,
and further characterization of the JNK function
linked to gene expression downstream of Fas will help
us understand the role of the...
...
Fig. 3. cDNA sequence and sequence alignment of Cyn d 24. (A) Nucleotide and deduced amino acid sequences of Cyn d 24. The numbers
on the right of the figure indicate the positions of the nucleotide ... to the N-terminal portion of the allergen.
Based on this partial sequence, two specific primers
were designed and used together with the anchor pri-
mer AP2 to...
... 2009 FEBS 4377
DNAi are the lower cost of dsDNA synthesis and the
lack of necessity for further DNA modification. These
observations may open a new path towards the use of
dsDNA or siDNAs as reverse ... when dsDNA corre-
sponded to the intron sequence of the target gene [24].
Hydrolysis of dsDNA with Dicer makes it possible
to channel the siDNA molecules into RISC b...
... On the other
hand, the HN cDNA shared a high degree of homol-
ogy with the histone H4 coding region (Fig. 3), but
lacked the first ATG codon necessary to translate the
whole histone H4 protein and ... determination of H4-v.1 and HN cDNAs
Determination of the length and sequences of H4-v.1
and HN mRNAs was accomplished by 3¢RACE and
5¢RACE experiments using a M...
... similarity with the other members of the IL-1
family; 30–38% with IL-1a, 26–36% with IL-1b and 30%
with IL-1Ra (data not shown). To further analyse the
relationship of trout IL-18 with the other ... extracted twice with
an equal volume of phenol/chloroform (24 : 1, v/v) and
once with chloroform. Genomic DNA was precipitated
with two volumes of cold etha...