Báo cáo khoa học: Aptamers toEscherichia colicore RNA polymerase that sense its interaction with rifampicin, r-subunit and GreB ppt
Báo cáo khoa học: Aptamers toEscherichia colicore RNA polymerase that sense its interaction with rifampicin, r-subunit and GreB ppt
... Aptamer probes of RNA polymerase structure (Eur. J. Biochem. 271) 4931 Aptamers toEscherichia colicore RNA polymerase that sense its interaction with rifampicin, r-subunit and GreB Andrey Kulbachinskiy1,2, ... when GreB w as added b efore the aptamers (Fig. 5 B a nd data not shown).Specific and nonspecific interactions of aptamers with the RNAP main channelThe interaction of the a ptamers with RNAP ... ligands that bindRNAP and stabilize alternative structural states of theenzyme could open the way for a better understanding ofthe conformational flexibility of RNAP. Aptamers are synthetic RNA...
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Tài liệu Báo cáo khoa học: Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity ppt
... howthe interaction between 14-3-3 and ExoS occurs,our pull-down analysis with GST-ExoS(LDL426–428AAA) still strongly suggests that ExoS must utilizea strategy for its interaction with 14-3-3 that ... mediatesthe interaction of 14-3-3 with the phospho-amino acidresidues in its interaction partners. Therefore it is likely that each dimer contains two binding pockets and caninteract with a single ... S residues that mediate the interaction with 14-3-3 and its biological activityLubna Yasmin1,*, Anna L. Jansson1,*, Tooba Panahandeh1, Ruth H. Palmer3, Matthew S. Francis2 and Bengt...
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Tài liệu Báo cáo khoa học: Activation of the Torpedo nicotinic acetylcholine receptor The contribution of residues aArg55 and cGlu93 ppt
... ion channel[1,2]. The a and b subunits of the Torpedo receptorreferred to in this report correspond to the a1 and b1subunits in the nomenclature recommended by theInternational Union of Pharmacology ... sites for agonists and competitive antagonists[4,5]. It is now generally agreed that these sites lie atthe interfaces between the a–c and the a–d subunits[6]. Labeling and mutational studies ... of the a-subunits (designated as loopsA–C, the ‘primary component’), together with aminoacids in the neighboring c and d subunits (lying inloops D–F, the ‘secondary component’) that partici-pate...
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Báo cáo khoa học: Weak toxin WTX from Naja kaouthia cobra venom interacts with both nicotinic and muscarinic acetylcholine receptors potx
... nicotinic and muscarinic ligands, suggesting that WTX interacts with both nAChR and mAChR. Experiments using hetero-logous expression of mAChRs revealed that the mech-anism of WTX interaction with ... membranes by muscarinic ligands demon-strates a specific interaction of WTX with mAChRs.WTX interaction with heterologously expressedmAChRsTo characterize the WTX interaction with mAChR inmore detail, ... ligand ACh binds) of nAChR, whereas its interaction with mAChR is allosteric.ResultsDistribution of WTX binding in mouse organsMice were injected intraperitoneally with radiolabeledWTX, and...
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Báo cáo khoa học: Identification of three proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand pdf
... humans and yeast [6–8] and its loss leads to genome i nstability and chromosomal end fusion through the activation of DNAdamage checkpoints [5,9].Proteins associated with both double-stranded and G-rich ... 3051 sense and antisense oligonucleotides, as described by Canoet al . [28]. Fully partial duplex and double-stranded DNA(dsDNA) were purified from the residual single-strandedDNA (ssDNA) and ... Pif1p, h as anuclear form that coimmunoprecipitates with telomericDNA in vivo and inhibits telomerase activity [44].MS analysis and the sequence tags m/ z 1339.7 and m/z1426.8 obtained by...
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Báo cáo khoa học: "Automatic training of lemmatization rules that handle morphological changes in pre-, in- and suffixes alike" docx
... part of the word. Its performance was compared with a stemmer using hand crafted stemming rules, the Euroling SiteSeeker stemmer for Swedish, Danish and Norwegian, and also with a stemmer for ... finite time. To keep the number of candidate rules within practical limits, we used the strategy that the pat-tern of a candidate is minimally different from its parent’s pattern: it can have ... rectly and those that do not match the pattern of the candidate. For each candidate rule the training algorithm creates a 2×3 table (see Table 2) that counts the number of training pairs that...
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Báo cáo khoa học: "A MODEL OF PLAN INFERENCE THAT DISTINGUISHES BETWEEN THE BELIEFS OF ACTORS AND OBSERVERS" pot
... stand on his head, and intends his doing the latter to play a role in his plan to do the former and that he also believes that he can talk to Kathy, believes that he can find out how to stand ... just that. And if R further believes that the system manager can override file permissions and that Tom is the system man- ager, but also that Q does not know the former fact, R will judge that ... that will explain why he thinks that standing on his head will lead to talking with Kathy. And, in the version of example (1) in which R believes that Q believes that Tom is a faculty member,...
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Báo cáo khoa học: Anti-(Raf-1) RNA aptamers that inhibit Ras-induced Raf-1 activation docx
... of RNA 9A; lanes 7, 8, and 9: 20, 100, and 200 pmol of RNA 9B.(B)Lanes 1and2 ,noRNA;lanes3,4,5,6,7 ,and8 ,10,20,40,60,80, and 100 pmol of RNA 9 A (C) Lanes 1 and 2, no RNA; l anes 3, 4, 5, 6,7, and ... we isolated and characterized RNA aptamers to the Raf-1 RBD, and inhibited the Ras-inducedRaf-1 activation by these aptamers. The aptamers, RNAs9A and 9 B, prevented t he RasáRaf-1 interaction ... eliminate the RNAs that bind GST. Prior tothe i ncubation with the target protein (in rounds 6±9) and after the elution (in rounds 8 and 9), the RNA pool wasincubated with 1 00 pmol of GST and a matrix...
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Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... (EMSA) with EWS and several G-quadruplex or single- or dou-ble-stranded DNA and RNA. Here, we show that EWS specifically targets G-quadruplex DNA and RNA in vitro. We also determined that the ... performed with EWS (lanes 2 and 4) and 32P-labeled ETS-1(lanes 3 and 4) or ssDNA L (lanes 1 and 2). (B) EMSA was per-formed with EWS (lanes 2, 4 and 6) and 32P-labeled Htelo (lanes 3 and 4), ... performed with RGG3 (lanes 2 and 4) and 32P-labeled Htelo (lanes 1 and 2) orrHtelo (lanes 3 and 4). (B) EMSA was performed with RGG3 (lanes2 and 4) and 32P-labeled rHtelo (lanes 1 and 2) or...
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Tài liệu Báo cáo khoa học: The double-stranded RNA-binding motif, a versatile macromolecular docking platform doc
... blue and grey) in complex with its physiological RNA target (blu ⁄ green and grey). Recognitionof the RNA A-form helix is mediated by contacts between by loops2 and 4 and the RNA minor and major ... in complex with an RNA hairpin [31] and the one of Escherichiacoli RNaseIII dsRBM in complex with dsRNA [35],confirmed the existence of a common interaction pat-tern in dsRBM–dsRNA recognition. ... separated interaction modes. These are the interaction of residues of the protein loops 2 and 4 with 2¢OH and phosphate groups of sequential minor and major grooves of the RNA helix, and the inter-action...
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