Báo cáo khoa học: Mimicking phosphorylation of the small heat-shock protein aB-crystallin recruits the F-box protein FBX4 to nuclear SC35 speckles docx

... Mimicking phosphorylation of the small heat-shock protein aB-crystallin recruits the F-box protein FBX4 to nuclear SC35 speckles John den Engelsman1, Erik ... desmin-related myopathy; phosphorylation; SC35; small heat-shock p rotein; ubiquitin isopeptide ligase. aB-crystallin is a member of the family of small heat-shock proteins [1–3]. In mammals, aB-crystallin ... appears that the localization of pseudophosphorylated aB-crystallin in nuclear speckles isnot dependent on intact DNA or RNA. aB-crystallin S19D/S45D recruits FBX4 to the SC35 speckles We have...

Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt

... threonine phosphorylation of tau [20,21]. To determine whether the tyrosine phosphorylation status of tau also affects the binding of tau to Fyn-SH3, CHO cells werecotransfected with Fyn together ... models of AD [8], suggesting that disruption of the association of these proteins could represent a poten-tial therapeutic strategy for the treatment of AD. Here,we used GST fusion proteins of ... reveal that the tyrosine phosphorylation status of tau is important formediating the association of these two proteins.Here, we used Chinese hamster ovary (CHO) cells to characterize the interaction...

Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-afﬁnity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt

... details of these signaling events have yet to beinvestigated, many of the molecular interactions down-stream of the Eph and ephrin molecules appear to lead to the regulation of the cytoskeleton of ... unknown whether the two classes of effectors can bind to B ephrins simulta-neously or if the binding of one affects the binding of the other. We report here that phosphorylation of Tyr304 in the functional ... known whether these twophosphorylated tyrosine residues contribute to high-affinitybinding of the ephrin B cytoplasmic domain to the Grb4adaptor protein [11]. The equivalent Tyr304 residue of Xenopus...

Báo cáo khoa học: Tyrosine phosphorylation of calponins Inhibition of the interaction with F-actin pot

... densitometric measurements of the autoradiograms using the Scan Analysis software of Biosoft(Cambridge, UK). The calponin phosphorylation in the presence of F-actin was conducted under the same ... change in the binding of either calponin to the modiﬁed actin. Thus, the actin-stimulated phosphorylation of calponins we observed wasnot the result of a nonspeciﬁc alteration of the actinstructure ... calponin, proteins participating in the reorganization of the actin cytoskeleton. Less well charac-terized is the tyrosine phosphorylation of calponins and itseffects on the actin-binding capacity of...

Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx

... the MARE [45–49]. The increased concentra-tion of endogenous heme may facilitate the binding of Nrf2, instead of Bach1, to the MARE to activate the MARE-dependent promoter, as reported for the mouse ... and HO-2 mRNA. The intensity of the signals representing HO-1 or HO-2 mRNA was normalized with respect to the intensity of the 18S rRNA signal. The ratio of each normalized value to the control ... 6). On the otherhand, knockdown of either HO-1 or HO-2 expressionresulted in the accumulation of heme in the hemin-treated HepG2 cells. Taken together with the siHO-2-mediated induction of HO-1...

Tài liệu Báo cáo khoa học: Major phosphorylation of SF1 on adjacent Ser-Pro motifs enhances interaction with U2AF65 doc

... GST beads. The binding of the different proteins to GST–SF1f wasquantified (bottom) as the fraction of the input protein (% of input) that was bound to the beads (the total input protein was ... dupli-cate. One per cent of the input was loaded to allow quantificationby phosphorimaging of the fraction of the 35S-labelled proteins thatwas bound to the beads. The mean values of duplicates with ... domain, suggesting that phosphorylation of these sites might regulate the formation of the SF1-U2AF-RNA complex. We first tested the effect of phosphorylation on the interaction of SF1 withU2AF65by...

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... cytometry revealed that the inhibition of cellgrowth was due to a decrease in the percentage of cellsin the S and G2 ⁄ M phases of the cell cycle (Fig. 3B).In a total of seven separate determinations, ... examined whether iron loading of the liver epithelial cell line HepG2 inﬂuenced the expression of immune regulatory molecules known to function as ligands of selected subsets of T cells, suchas MHC ... iron-loaded hepatocytes may facili-tate phagocytosis and removal of the purportedlyapoptotic cells by resident macrophages through the phosphatidylserine receptor [54]. Removal of apoptoticcells...

Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx

... concerted proton transfer to the leaving group of the substrate occurs.Proton transfer The requirement of an acceptor for the Oc proton from the catalytic serine is a critical component of the mechanism ... the carbonyl carbon of the substrateundergoes a nucleophile attack by the Oc of the catalyticserine, while the proton is shuttled to the catalytic histidine.This results in the formation of ... Compression of the His-Asp/Glu diad causes the basicity of the histidine to increase, so that it is able to accept/abstract the Oc proton from the catalytic serine.Transferring a proton from the catalytic...

Báo cáo khoa học: New application of ﬁreﬂy luciferase ) it can catalyze the enantioselective thioester formation of 2-arylpropanoic acid docx

... respectively. The afﬁnity of keto-profen was almost the same of d-luciferin. In addition, the calculatedE-value toward ketoprofen was approximately 20. These results suggestthat LUC-H could catalyze the ... anability to distinguish the absolute conﬁguration of the starting material, the ee of the compounds in the twolayers will increase.After the above mentioned separation procedure, the ee of the materials ... addi-tion, the enatiomer ratios of these compounds were par-tial to the R-form. These results give support to the results of the HPLC analysis of unreacted acids, whichindicate that the thioester...

Báo cáo khoa học: Functional characterization of front-end desaturases from trypanosomatids depicts the ﬁrst polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan ppt

... eachdesaturase and to depict, for the ﬁrst time, the com-plete pathway for PUFA biosynthesis in a protozoon.Moreover, it would help to resolve an old controversyrelated to the isomeric nature of the PUFAs ... con-sidering the complexity of their life cycles, where theyhave to adapt to dramatic changes of temperature andmorphology. It may be very interesting to evaluate the possibility of using this ... selectable marker gene in thisvector confers uracil prototrophy to the host. Either the vector alone, or the vector harbouring different desaturases,was used to transform S. cerevisiae strain...

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