Báo cáo khoa học: Identification of a copper-repressible C-type heme protein of Methylococcus capsulatus (Bath) A member of a novel group of the bacterial di-heme cytochrome c peroxidase family of proteins docx
... a thermal heating block and cooling to 4 C at a rate of 2 C min
–1
.
Name Sequence
ssDNAS d(CATTCCCACCGGGACCACCAC)
ssDNA L d(CATTCCCACCGGGACCACCACCATTCCCACCGGGACCACCAC)
ETS-1 d(TCTCTCGGTGGCCGGGGCTCGTCGGGGTTTTGGGTCCGTCC)
Htelo ... TCA TGG CGT CCA CGG ATT ACA G)
and EWS reverse d(CGC TCG AGT CAC TAG TAG GGC
CGA TCT CTG C) , for pGEX–EWS; EAD forward d(CGG
AAT TCA TGG CGT CCA CGG ATT ACA...
... study
asd1 AAG CCG ATG ACA CCA ATT (asd sense) This study
asd2 GCA GGT TCA TAG TGC ATG (asd antisense) This study
Kan RP GGT GCG ACA ATC TAT CGA (kanamycin sense) [19]
Kan FP CTC ATC GAG CAT CAA ATG ... study
ats1 GCT CAA TCC GTG ATG TGA (ats sense) This study
ats2 CGA CTG CAC TGA TGA GCT (ats antisense) This study
lg1 CTT CAA GCC ATG TCA AAG (lgt6 sense) This study
lg2 CGA ATA ATC ATC ACA CT...
... substances may be aggregates of
proteins and polysaccharides, and may play important
roles in formation of the phases and ⁄ or morphologies of
the crystals because they consist of acidic glycoproteins
and ... which may comprise proteins and
heparan sulfate glycosaminoglycan chains, although
the structures of the proteins and the glycosaminogly-
can chains were not ch...
... in
the mammalian kidney [2,4,5] and plant roots [6,7]. The
yeast Saccharomyces cerevisiae has four such MIP channels:
the aquaporins Aqy1 and Aqy2 and the aquaglyceroporins
Fps1 and Yfl054 [8]. Aqy1 ... (MIP) family] have been found in archea,
eubacteria, fungi, plants, animals and human [2,3]. Aqua-
porins facilitate the diffusion of water across biological
membranes while the...
... )525
(5¢-TGACCTTGTCTCGTTGCCTCACCC-3¢)and)378
(5¢-GCTACAGGGATGCCAAAAGAACCC-3¢)forthe
Site-3, and primer set )346 (5¢-GCGTCTCACCCTAGT
CCTGGTCCTGC-3¢)and)214 (5¢-GGAAGGGGCGGG
TCCAGAGAACA-3¢) for the Site-2 element. PCR was
performed ... DNAs
used in EMSA analysis were: NF1 wt, 5¢-TTTTG
GATTGAAGCCAATATGATA-3¢;NF1mut,5¢-TTTT
GGATTGAATAAAATATGATA-3¢;Site-2wt,5¢-GCGT
CTCACCCTAGTCCTGGTCCTGCTCCAAGGG...
... of this novel structural feature identified a
further degree of variability within the inner-core oligosac-
charide of meningococcal LPS which may contribute to the
interaction of meningococcal ... meningococcal clinical isolates revealed an
additional and uniquely located glucose residue in the core
oligosaccharide that had not previously been identified in
meningococcal LPS....