Báo cáo khoa học: Signalling and regulation of collagen I synthesis by ET-1 and TGF-b1 doc

Tài liệu Báo cáo khoa học: Oxygen-dependent regulation of hypoxia-inducible factors by prolyl and asparaginyl hydroxylation pdf

... analysis of HIF-1a and HIF-2a protein stability and transactivation potencythat have provided the greatest insights into oxygen sensing and regulation. HIF protein stabilityInitial biochemical ... basicDNA binding region and HLH primary dimerization inter-face. The adjacent PAS domain is comprised of approxi-mately 300 amino acids and is subdivided into two semiconserved repeat regions ... Likewise, analysis of HIF-2a revealed that it also contained two transactivationdomains with similar organization [67,79] and, as with HIF-1a,theCADofHIF-2a was also inducible by hypoxia.While...

Báo cáo khoa học: Signalling and regulation of collagen I synthesis by ET-1 and TGF-b1 doc

... affectedsimilarly, with no effect by inhibiting PLC-b (Fig. 5A),strong down -regulation of collagen I levels after inhi-bition of PC-PLC (Fig. 5B), and similar collagen I down -regulation after PLD inhibition ... strong inducer of collagen I synthesis [3,14]. Further investigations should reveal if ET-1 eli-cits induced collagen I synthesis solely via induction of CTGF or via additional mechanisms as it is ... involvement of PC-PLC and PLD in the signalling pathways elicited by ET-1 and TGF-b1. ET-1 and TGF-b1 each stimulated collagen I production and in an additivemanner. ET-1 further induced connective tissue...

Báo cáo khoa học: Fatty acid regulation of adenylyl cyclase Rv2212 from Mycobacterium tuberculosis H37Rv doc

... exceptional vari-ability in basal enyzme activities. Although in theholoenzyme this variability was greatly reduced by addition of linoleic acid, the extent of stimulation wasdiminished in protein ... mixture of conformations of different speciﬁc activities, and that (b) fatty acidsFig. 4. Kinetic analyses of the stimulation of Rv22121)388 by 100 lM linoleic acid (A) and by 170 lM polidocanol ... respectively; that is, the variability of basal and stimulated activities in individual proteinpreparations were identical (54%; Fig. 3B). Thus, thelesser activation of the catalytic domain Rv2212212)388by...

Báo cáo khoa học: Template-independent ligation of single-stranded DNA by T4 DNA ligase doc

... circularized I linear, we hybridized oligonucleotide H17 to the DNAsegment of I encompassing the recognition sequence of HhaI before restriction digestion. With this modifica-tion, essentially a single ... 10)5n.d.Fig. 4. Investigation of ssDNA ligation activity of different DNAligases. Oligonucleotide I (10 nM) was incubated with ligase,cleaved by HhaI, and subjected to PCR amplification. Ligases ... absence of I or with 10 nM of I in the absence of ligase,respectively.ABFig. 6. Determination of ligation yields by qcPCR. (A) Co-amplifica-tion of 8 fmol I, which had previously been incubated...

Tài liệu Báo cáo khoa học: Small molecule regulation of Sir2 protein deacetylases ppt

... activator of Sir2 activity [41].Isonicotinamide was shown to directly compete withnicotinamide for binding. Nicotinamide is a potentinhibitor of the Sir2 reaction because of its aforemen-tioned ... adenine mimics. If this is the case,then it is likely that they possess activity against otherNAD+binding enzymes. This effect is seen with nico-tinamide, which in addition to its Sir2 inhibitory ... is the relief of the inherentnicotinamide inhibition by competition with isonicotin-amide, which does not readily react with the enzymeintermediate. Although the K i for isonicotinamide was68...

Tài liệu Báo cáo khoa học: "The Eﬀect of Corpus Size in Combining Supervised and Unsupervised Training for Disambiguation" pdf

... Supervised and Unsupervised Training for DisambiguationMichaela AttererInstitute for NLPUniversity of Stuttgartatterer@ims.uni-stuttgart.deHinrich Sch¨utzeInstitute for NLPUniversity of Stuttgarthinrich@hotmail.comAbstractWe ... attachment possibilitiesbased on pointwise mutual information, thewell-known measure of how surprising it is tosee R and X together given their individualfrequencies:MI(< R, i, X > ) ... of dependen-cies (a piece of a tile of a roof of a house vs.a piece of a roof of a tile of a house) cannotbe distinguished. We believe that an invertedindex is the most eﬃcient data structure...

Tài liệu Báo cáo khoa học: "DiMLex: A lexicon of discourse markers for text generation and understanding" docx

... explicitly assign such categories. The part of speech of a marker (conjunctive, subordinating conjunction, coordinating conjunction, preposition) determines the possibil- ities of positioning ... detailed studies of individual groups of markers, such as [Vander Linden, Martin 1995] for PURPOSE markers. Besides, the Linguistics literature offers fine-grained analyses of individual ... developing a Discourse Marker LEXicon (DiMLex), which aims at assembling the various information as- sociated with markers and describing it on a uniform level of representation. Our initial...

Báo cáo khoa học: Calmodulin-mediated regulation of the epidermal growth factor receptor doc

... recruit-ment of adaptor and signalling proteins, initiate multi-ple signalling events [16–20]. The EGFR is implicatedin the control of cell proliferation and differentiation,cell survival, ... in an inactive but pre-dimerized ⁄oligomerized state, which is subsequently activatedafter ligand binding by inducing the rotational reorga-nization of both monomers, with such an activationmechanism ... CaM-LDsuggest that this region might not be involved in theallosteric regulation of ligand binding afﬁnity in thereceptor [86].Interestingly, the occurrence of in-frame tandemduplication of exons 18–25...

Báo cáo khoa học: Alternative splicing: regulation of HIV-1 multiplication as a target for therapeutic action docx

... understanding of infection, spread and dissemination through splicing regulation. Such stud-ies coupled with the testing of splicing inhibitors should help the develop-ment of new therapeutic antiviral ... infec-tious particles. IDC16 inhibits replication of macro-phage- and T cell-tropic laboratory strains, clinicalisolates and strains with high-level resistance to inhibi-tors of viral protease and reverse ... approaches aimed at reducing the viral loadin patients infected by HIV utilize molecules intended toinhibit the enzymatic activity of viral reverse transcrip-tase or of the protease involved in virus...

Báo cáo khoa học: Small-molecule modulators of zymogen activation in the ﬁbrinolytic and coagulation systems pptx

... acidic and five aliphatic amino acids), iturin As(heptapeptide consisting of six polar amino acids and a proline) and iturin Cs (heptapeptide consisting of five polar and an acidic amino acids as ... activity [62]. Thus,the reciprocal activation of scu-PA and plasminogenconstitutes a mechanism of localized initiation and propagation of pericellular proteolysis [63–66] and fibri-nolysis ... Unlike lysine-bindingsites in K1, K2 and K4 of plasminogen, the AH sitecan bind an internal lysine residue in addition to theC-terminal lysine [30], which is a preferred ligand forthe lysine-binding...

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