Báo cáo khoa học: Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex docx

Báo cáo khoa học: Activation of PMCA by calmodulin or ethanol in plasma membrane vesicles from rat brain involves dissociation of the acetylated tubulin/PMCA complex pdf

... types of cells. H+-ATPase of Saccharomyces cerevisiae is similarly inhibited by interaction with acetylated tubulin. The activities of both these ATPasesare restored upon dissociation of the ... Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex. FEBS J 272, 5742–5752.6 Beltramo DM, Nun˜ez M, Alonso AC & Barra HS (1994) The relationship ... identify the isoforms that form complexes for each of the P-ATPases,to characterize more precisely the ‘ATPase ⁄ acetylated tubulin complex , and to identify the interactingdomains of the ATPase...

Báo cáo khoa học: Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex docx

... Campetelli et al. Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex Alexis N. Campetelli1, ... at the periphery. This is further evidence that glucose induced dissociation of the H+-ATPase–acetylated tubulin complex. H+-ATPase activity of isolated plasma membrane is inhibited by tubulin Plasma ... thatbesides dissociation of the complex, the enzymerequires some additional, time-consuming process forits activation. Anyway, the important conclusion fromthese experiments is that dissociation of the...

Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf

... cleavage of the scissile bond. This would affect FX and peptidehydrolysis similarly, supported by the similar decreasein the rate of cleavage of both types of substrates. The effect of the G372(223)A ... 296(154) [14–17]. The high speciﬁc activity of theseFVIIa variants is presumably linked to a more stableburial of the N-terminus of the protease domain in the activation pocket of the activation domain ... applied the technique of N-terminal pegylation to visualize the relative sol-vent exposure of the N-terminus of FVIIa. To the best of our knowledge, this is the ﬁrst time that this tech-nique has...

Tài liệu Báo cáo khoa học: Activation of the Torpedo nicotinic acetylcholine receptor The contribution of residues aArg55 and cGlu93 ppt

... location. The AChBP, which is secreted by the glial cells of the snail, Lymnaea stagnalis , is a truncated homologue of the extracellular amino terminal domains of the nAChR[see 10]. Inspection of its ... on the WT recep-tor are consistent with such a mechanism. The mostparsimonious explanation of the loss of the potentiat-ing effect in the mutant receptor is that the mutationresults in the ... contribute to the binding of agonists [23]. In the GABAAreceptor, the F64L mutation of the a1subunit had a dramatic effect on GABA sensitivity[24] and mutations of the F77 residue of the c2 subunitsigniﬁcantly...

Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc

... elegans is the best genetic model. The generation of transgenic C. elegans has been established and is easier than that of other animal models [35]. The results of this study indicate that the Gai ... indicating that the side effect of the acti-vation of GAR-3 is limited. Furthermore, the pheno-type of gar-3 loss -of- function mutants is almost wild-type with the exception of a faster pharyngeal pumpingrate ... GOA-1 is 82.2% identical tothat of Gao. Although the similarity of the aA–aEregion and the region upstream of a4 was relativelylow, the N-terminal region (aN–a1), the aF–aG regionand the...

Báo cáo khoa học: Activation of activating transcription factor 2 by p38 MAP kinase during apoptosis induced by human amylin in cultured pancreatic b-cells ppt

... apparent. p38 kinase activation is related to the presence of ﬁbrillogenic hA, and hA-induced activation of the p38 kinase pathway in b-cells is consistent with the general role of the p38 kinase pathway ... content.ATF-2 is a transcription factor whose activation canbe catalyzed by either p38 or JNK, or by both[27,41]. To further clarify the role of the MAPKs inhA-evoked activation of ATF-2, we ... death,depending on the duration of activation and the bal-ance of activity between the MAP kinase, ERK, and the stress-activated kinases, JNK and p38 [26]. Activation of MAPK -mediated signaling...

Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx

... Thus, the mechanisms involved in the regula-tion of gluconeogenic enzymes are very controversial,and it is thereby hypothesized that both of these fac-tors contribute to insulin action on glucose ... status within the cellin the given experimental conditions. Foxo1, anothermember of the forkhead family of transcriptionfactors, is a very well-established mediator of the effects of insulin ... insensitive by TNFa, there is a relativelyincreased nuclear translocation of Foxa2, we studied the resulting effects of TNFa pretreatment on the effect of insulin on the expression of PEPCK and another...

Báo cáo khoa học: Activation of Stat5 and induction of a pregnancy-like mammary gland differentiation by eicosapentaenoic and docosapentaenoic omega-3 fatty acids docx

... preventative effect of a dietary supplement of n-3 PUFAs to the mother on the risk of breast cancerrisk for offspring has been reported. Offspring of the rat fed an n-6 PUFA diet during pregnancy ... interpretation of the data is limited by signiﬁcant differences in the methods used to ascertain exposure to n-3 PUFA.Third, of particular note is the fact that n-3 PUFAconsumption generally consists of ... considered. It is assumed thatmost of the beneﬁcial effects including cancer preven-tion is mediated by alternation of the n-3 ⁄ n-6 composi-tional ratio but not the exact amount of n-3 PUFA[24–27]....

Báo cáo khoa học: Kinetic mechanism for p38 MAP kinase a A partial rapid-equilibrium random-order ternary-complex mechanism for the phosphorylation of a protein substrate potx

... for the second-order rate con-stant of ka¼ 104m)1Æs)1for the binding of MgATP top38 MAPKa gives a rate constant for the dissociation of MgATP from the enzyme of k-a¼ 3.5 s)1, if the ... hands because the activity of the enzyme was also affected by the presence of Triton.Isothermal titration calorimetryTo lend further support to our conclusions, weexamined the binding of ADP and ... AMP-PCPto p38 MAPKa in the presence and absence of ATF2D115 by isothermal titration calorimetry (ITC).ITC is the most direct method for the determination of macromolecular ligand dissociation constants...

Báo cáo khoa học: Activation, regulation, and inhibition of DYRK1A Walter Becker1 and Wolfgang Sippl2 pptx

... work is required to dissect the full spectrum of activation modes of the different CMGC group kinas-es. However, so far there is no evidence that the cata-lytic activity of any member of the ... inhibitor of CLKs. Obvi-ously, the promiscuous behaviour of these inhibitorslimits their value as tools for the analysis of signallingpathways. Nevertheless, use of purvalanol A has guided the identiﬁcation ... by DYRK1A overexpression [12]. Furthermore,cross-reactivity with other kinases is not an issue inmechanistic studies with puriﬁed enzymes, such as the analysis of the autoactivation mechanism...

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