Báo cáo khoa học: Comparative biochemical characterization of nitrile-forming proteins from plants and insects that alter myrosinase-catalysed hydrolysis of glucosinolatesk docx
... from plants and insects M. Burow et al.
2446 FEBS Journal 273 (2006) 2432–2446 ª 2006 The Authors Journal compilation ª 2006 FEBS
Comparative biochemical characterization of
nitrile-forming proteins ... characterization of
nitrile-forming proteins from plants and insects that alter
myrosinase-catalysed hydrolysis of glucosinolates
Meike Burow, Jan...
... The NOE
peaks between residues of the binding loop and that of the
11±16 loop are also d ifferent from that of SGCI and its
variant (Table 3).
Qualitative evaluation of H±D exchange data
Proton±deuterium ... properties o f t he amide protons
of SGCI[L30R, K31M] are very similar to that of SGCI.
Both the amide proton of Lys13 of SGCI and of
SGCI[L30R, K31M] vari...
... Rao and Dr J.
Josse for critical review of the manuscript, and Profes-
sor W. Lo
¨
ffelhardt (University of Vienna, Austria) for
his assistance in N-terminal protein sequencing and
some MALDI-TOF ... sPPases of photosynthetic bacteria,
a polyphyletic and very diverse assembly of prokaryo-
tes, are worth characterizing.
An increasing body of biochemical and genetic evi-
d...
... elements that are adopted only temporarily
and ⁄ or in a fraction of the protein population.
Recent data suggest that even proteins that adopt a
well-defined structure by conventional standards ... observed and described for
lentiviral Tat [32,45–52] and Vpr [53–56]. A well-
defined and rigid tertiary structure of these proteins is
observed only in complexes with one of...
... Fe -proteins or other proteins.
Results and Discussion
Site-directed mutagenesis of the target arginine
of dinitrogenase reductase
An indication for the covalent modification of one
subunit of ... shift of 541.06 m ⁄ z. Signals from the
singly and doubly charged b- and y-ion ser-
ies, as well as ions from the fragmentation
of the post-translational modification, are
ind...
... over-expression of the three proteins was signifi-
cantly associated with the genesis and progression of
CRC. The functional implications of the alterations in
the levels of these proteins are discussed ... and
metastasis-associated proteins in CRC. The results sug-
gest that RhoGDI, S100A9 and LASP-1 may play an
important role in the development and progression of
CR...
... upgrade
of existing genres and the creation of novel
genres.
The change of genre repertoire and the
creation of new genres were well illustrated by
Crowston and Williams (2000) and Shepherd and ... generated from specific
needs and requirements of the web. Crowston
and Williams (2000) have traced this evolution
through a manual qualitative survey of 1000 web...
... languages was
obtained by Thatcher and Wright (1968) and
Doner (1970) who established that the CFLs
were all and only the sets of strings forming the
yield of sets of finite trees definable ... Doner's and Thatcher and Wright's proofs
that the recognizable sets of trees are char-
acterized by definability in wSnS lift directly
to a proof that the recogn...
... Dolce
1
,
Ferdinando Palmieri
3
and Loredana Capobianco
2
1 Department of Pharmaco-Biology, University of Calabria, Arcavacata di Rende, Cosenza, Italy
2 Department of Biological and Environmental Sciences and ... Dolce,
Department of Biological and Environmental
Sciences and Technologies, University of
Salento, 73100 Lecce, Italy; Department of
Pharmaco-Biology, Univers...
... protein in
E. coli and studied its substrate preferences, the factors
influencing its activity and the nature of suitable metal
cofactors and inhibitors in vitro.
Materials and methods
Chemicals
The ... mito-
chodrial AOX activity and proposed that the effect of
organic acids could be due to the fact that they chelate metal
cations. We did observe an inhibitory effect of bo...