Báo cáo khoa học: Novel b-1,3-, 1,6-oligoglucan elicitor from Alternaria alternata 102 for defense responses in tobacco docx
Báo cáo khoa học: Novel b-1,3-, 1,6-oligoglucan elicitor from Alternaria alternata 102 for defense responses in tobacco docx
... 2006)doi:10.1111/j.1742-4658.2006.05249.xA novel elicitor that induces chitinases in tobacco BY-2 cells was isolated from Alternaria alternata 102. Six other fungi, including A. alternata IFO6587, could not induce, or weakly induce ... genes. In comparison with laminarin and laminarin sulfate, which are reported tobe potent elicitors of defense responses in tobacco, the expression patternof genes induced by the purified b-1,3-, ... PCR wasperformed with denaturing, annealing and extensiontemperatures of 94 °C for 0.5 min, 50–58 °C for 0.5 minand 72 °C for 0.5 min, respectively, for 24–34 cycles. Theannealing temperature...
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Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx
... detected, indicating that calpainhad undergone a transition from the low to the highaffinity mAb-binding form. Interestingly, these resultsobtained in vivo were almost superimposable on thoseobtained ... the intensity of calpain staining increased 7–8-fold, indicating that the protease was now mainlypresent in the high-affinity form. In these conditions, although the highest amount ofcalpain ... not only indicate that calpastatin inducesa pronounced Ca2+-independent change in calpainconformation, but also provide strong support for pre-vious observations indicating that calpain and...
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Báo cáo khoa học: "SITS: A Hierarchical Nonparametric Model using Speaker Identity for Topic Segmentation in Multiparty Conversations" pptx
... incorporating a participant-level social component. Specifically, we explicitlymodel an individual’s tendency to introduce a topic.After outlining inference in Section 3 and introducingdata in Section ... topical trends. In Knowledge Discoveryand Data Mining, Knowledge Discovery and Data Min-ing.87Proceedings of the 50th Annual Meeting of the Association for Computational Linguistics, pages ... outperform TextTiling. In addition, amongthe probabilistic models, the models that had accessto speaker information consistently segment betterthan those lacking such information, supporting...
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Báo cáo khoa học: The gene carD encodes the aldehyde dehydrogenase responsible for neurosporaxanthin biosynthesis in Fusarium fujikuroi potx
... of1mLÆmin)1, with a linear gradient from 100% B to 43% Bwithin 45 min, and then to 0% within 1 min, with the finalconditions being maintained for another 24 min at a flowrate of 2 mLÆmin)1. ... trans-formant strains were named T3 and T4.The molecular integrity of carD was investigated in T3 and T4 strains, in two nonsectoring transformants,T1 and T2, and in the SF134 original strain. ... domain is shaded in gray. The TMdomain of YLO-1 is shaded in black, and theequivalent sequence in CarD is boxed. Thetwo amino acid changes found in thisprotein segment in F. graminearum CarD(FGSG09960,...
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Báo cáo khoa học: Salicylic acid and the hypersensitive response initiate distinct signal transduction pathways in tobacco that converge on the as-1-like element of the PR-1a promoter pot
... C. (2000) Tobacco transcription factor TGA2.2 is the main component of as-1-binding factor ASF-1 and is involved in salicylic acid- and auxin-inducible expression of as-1-containing target ... coordinated induction of aheterogeneous group of proteins in the infected anduninfected leaves, commonly referred to as pathogenesis-related (PR) proteins.PR proteins were first described in tobacco ... proteins are induced to high levels in necrotic tissue from tobacco plants expressing thenahGgene from P. putidaIt is envisaged that PR-1 gene induction in local andsystemic tissues of infected...
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Báo cáo khóa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis Evidence for independent mutarotation site pdf
... beenmapped in detail, and involvement of several amino acids,e.g. cysteines [17,26,32], histidine [33] and arginine [34], hasbeen suggested. Apart from these, Ôreductive inhibitionÕ wasperformed ... observed using the couplingenzyme glucose oxidase. The reactions followed linearkinetics up to 2 min (Fig. 2B). The initial rates were foundto be related to epimerase concentration in a linear ... 6.8 and 91% for capsicum mutarotase. In another set, refolding wasinitiated in the absence of extraneous NAD. In this case, norecovery for epimerase was observed without affecting therecovery...
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Báo cáo khoa học: "Extracting Key Semantic Terms from Chinese Speech Query for Web Searches" ppt
... known phrases in orderto furthereliminatetheerrors.Theresultingsystemhasbeenfoundtobeeffective.1 IntroductionWeareentering aninformation era, where infor-mationhasbecomeoneofthemajorresources in ourdailyactivities.Withitswidespreadadoption,Internethasbecomethelargestinformationwealth for ... wouldenableaveragepersonstoaccessinformationusingthecurrentsearchengineswithouttheneedtolearnspecialcomputerskillsortraining.Theycansim-ply access the search engine using common de-vices ... QueryTermsGenerationBecauseofspeechrecognitionerror,theCSSob-tained is likely to contain error, or in the worsecase,missingthemainsemanticsofthequeryalto-gether.Wenowdiscusshowwealleviatetheerrors in CSS for theformercase.WewillfirstbreaktheCSS...
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Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt
... DNA-binding domain (DBD)at the amino-terminus and the oligomerization domain in the central region of the protein [1,4]. The HSFDBD belongs to the ‘winged’ helix-turn-helix family ofDNA-binding ... affinity-bind-ing sites for HSF.The DBD–DBD interaction is important in theHSF–HSF and HSF–HSE interactions. In HSF DBD–HSE co-crystals, the protein–protein interface consistsof the helix 2 amino-terminus, ... TheHR-A ⁄ B is indispensable for binding of a single tri-mer to three-unit HSEs and for HSF hyperphosph-orylation, but HR-A ⁄ B is not required for bindingHSEs with four or more units or for transactivationat...
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Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
... 3729–3734.Supporting informationThe following supplementary material is available:Fig. S1. Amino acid alignment of linker region andligand-binding domain (LBD) of ecdysone receptors.Fig. S2. Amino acid ... well-describedecdysteroid receptors from two insect species for whichrelevant biochemical and in vivo information exists.The comparative profiles demonstrate an approach for developing a screening system to identify ... of a hemipteran ecdysone receptor ligand-binding domain: comparison with a lepidopteranecdysone receptor ligand-binding domain andimplications for insecticide design. J Biol Chem 280,22258–22269.14...
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Tài liệu Báo cáo khoa học: Novel aggregate formation of a frame-shift mutant protein of tissue-nonspecific alkaline phosphatase is ascribed to three cysteine residues in the C-terminal extension pdf
... IgG) from Molecular Probes, Inc. (Eugene, OR,USA); antipain, chymostatin, elastatinal, leupeptin andMG-132 (benzyloxycarbonyl-l-leucinyl-l-leucinyl-l-leucinal)and pepstatin A from Protein Research ... cells were preincubated for 0.5–1 h in the methionine ⁄ cysteine-free DMEM and labeledwith 50–100 lCi of [35S]methionine ⁄ cysteine for 0.5 h in thefresh methionine ⁄ cysteine-free MEM. After ... Cytohistochemical staining for alka-line phosphatase. Cells expressing TNSALPor TNSALP (1559delT) were stained for alka-line phosphate activity in the absence orpresence of saponin.K. Komaru et al. Novel...
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