Báo cáo khoa học: Synthesis and structural characterization of a mimetic membrane-anchored prion protein doc

... compilation ª 2006 FEBS 1299M. R. Hicks et al. Lipid-anchored PrP Synthesis and structural characterization of a mimetic membrane-anchored prion protein Matthew R. Hicks1, Andrew C. Gill2, Imanpreet ... surface and an endocyticcompartment in cultured neuroblastoma cells. J BiolChem 268, 15922–15928.19 Naslavsky N, Stein R, Yanai A, Friedlander G & Tara-boulos A (1997) Characterization of ... 429–444.25 Naslavsky N, Shmeeda H, Friedlander G, Yanai A, Futerman AH, Barenholz Y & Taraboulos A (1999)Sphingolipid depletion increases formation of the scra-pie prion protein in neuroblastoma...

Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deﬁcient families from Southern Italy docx

... S, Dianzani I, Lattanzi P, Spada M,Romano V, Calı`F, Andria G, Ponzone A, Marra E &Piazza A (2001) Genetic heterogeneity in ﬁve Italianregions: analysis of PAH mutations and minihaplo-types. ... Italy. The study was partly supportedby Agenzia Italiana del Farmaco (AIFA grantFARM5MATC7), Rome, Italy. We thank Jean AnnGilder for revising and editing the text and AnnaNastasi for her skilful ... Giancarlo Parenti4, Luciana Esposito6, Adriana Zagari1, Generoso Andria4 and Francesco Salvatore1,21 CEINGE–Biotecnologie Avanzate Scarl, Naples, Italy2 IRCCS – Fondazione SDN, Naples,...

Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx

... 2495Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylasefrom the Archaeon Pyrococcus furiosusGiovanna Cacciapuoti1, Sabrina Gorassini1, Maria Fiorella Mazzeo2, ... (time-zero control) and at different time inter-vals, aliquots were removed from each sample and analyzedfor activity in the standard assay. Activity values areexpressed as a percentage of the zero-time ... City, CA, USA), as alreadydescribed [57]. In the m ⁄ z range 4000–40000 mass spectrawere acquired in linear positive-ion mode and calibratedusing as internal standards the average double and...

Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx

... homodimers and dissociates in membrane and solublesubcomplexes; isolation of the Ndh complexby BN/PAGE and Tricine/PAGEIntact MS and BS chloroplasts were used as startingmaterial for the separation ... chloroplastswere separated in CN ⁄ PAGE (1D, the hori-zontal bands) and then in nondenaturingconditions by BN ⁄ PAGE (2D). The molecularmass standards and direction of migrationare indicated ... essential forthe synthesis of ATP. Homologs of this complex existin bacteria, the mitochondria of eukaryotes, and thechloroplasts of plants. The bacterial and mitochondrialKeywordschloroplast;...

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... putative allantoate amido-hydrolase, which is part of the urate catabolic pathwayin many organisms [8]. In fact, by genome data mining,another hydantoinase (HYD) was also found in theJannaschia ... (1988)Stereo- and substrate-speciﬁcity of a d-hydantoinase and a d-N-carbamyl amino acid amidohydrolase of Arthrobacter crystallopoietes AM 2. Enzyme MicrobTechnol 10, 618–625.12 Ogawa J & Shimizu ... weight proteins. The ﬂowrate was set to 0.8 mL Æmin)1. The subunit molecular masswas also estimated by SDS–PAGE. Characterization and comparative analyses of HYDJs and HYDBpThe optimal temperature...

Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... developmental cycle: early, middle and latesubstrate colonization stages (4, 8 and 12 days); pinheadstage (day 14), button stage (day 18), egg stage (day 21),elongation stage (day 22) and mature stage ... cycles of 94 °Cfor20s,52°Cfor20s and 70 °C for 2 min; then a ﬁnal extension at 72 °Cfor 10 min. The primers for lac1 PLAC1F(5¢-AGCTTTCATTCCCAGTGATTG-3¢)andPLAC1R(5¢-AACGAGCTCAAGTACAAATGACT-3¢) ... serially diluted lac1 and gpd template DNA. A clear linear relationship between the amount of templateinputs and PCR ampliﬁcation was obtained for both lac1(Fig. 3: right panel) and gpd (data...

Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

... 5¢-CTAGACTCGAGCCTAATTTATATTTGCTCCTTGTGC-3¢. b-Actin primers weredesigned as follows: forward 5¢-CTACAATGAGCTGCGTGT-3¢ and reverse 5¢-AAGGAAGGCTGGAAGAGT-3 ¢.Cell survival and apoptosis analysisFor viability ... regulation of caspases. Takentogether, these results indicate that VBARP is a novel splice variant of ANKHD1 and may play a role in cellular apoptosis (antiapoptotic) and cell survival pathway(s).AbbreviationsANK, ... theamount of protein used in this assay indicating that anequal amount of protein was used in all our samples and that the lack of interaction between VBARP and Nef is not due to the lack of...

Báo cáo khóa học: Overexpression and enzymatic characterization of Neisseria gonorrhoeae penicillin-binding protein 4 ppt

... of 90 min.D-Ala product was detected with QuantaBluTMassays. j,Boc-Cbz-KAA; r, Boc-Ac-KAA; s, Ac-C-KAA; n, Ac2-KAA; h,Boc-H-KAA; d, Ac2-KA-D-Lac. (A) High activity range showingactivity ... TEM b-lactamase, EC PBP 5, NG PBP 3, and NG PBP 4. NA, data not available; AA, amino acid.pK a EnzymeTEM b-Lactamase EC PBP 5 a NG PBP 3bNG PBP 4cpK a value AA residue pK a value AA residue ... [27].NG PBP 4 activity against the $D-Ala-D-Ala substrateAc2-KAA was fairly typical for a PBP with a kcatvalue of 3s)1, Kmvalue of 3 mM, and kcat/Km of 80M)1Æs)1(Table 2). NG...

Báo cáo khoa học: Biochemical and structural analyses of a higher plant photosystem II supercomplex of a photosystem I-less mutant of barley pot

... violaxan-thin, zeaxanthin and b-carotene, 3 for lutein and Chl b, and 7 for total carotenoid content (Tot Car). LL, low light; HL, high light; VAZ, violaxan-thin, antheraxanthin and zeaxanthin.Chl ... monomeric antenna proteins(CP26, CP29 and LHCII monomers) (band 2), and tri-meric LHCII (band 3), monomeric (band 4) and dimeric (band 5) PSII cores. Bands 6 and 7 containedsupramolecular complexes ... proﬁle of bands 6 and 7 from the WT and mutant (Zb63).Photosystem I (PSI) core major polypeptides(PsaA and PsaB), ATPase subunits and PSIIantenna polypeptides (Lhcb) are indicated,as identiﬁed...

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... development and use of N-acylsulfonamides and sulfonimides as antagonists of nucleic acid-bindingproteins.Database Structural data for the two RNase A complexes are available in the Protein Data Bank ... Functional and structural analyses of N-acylsulfonamide-linked dinucleoside inhibitors of RNase A Nethaji Thiyagarajan1, Bryan D. Smith2,*, Ronald T. Raines2,3 and K. Ravi Acharya11 Department ... USAIntroductionUpon catalyzing the cleavage of RNA, RNases operateat the crossroads of transcription and translation.Bovine pancreatic RNase A (EC 3.1.27.5) is the bestcharacterized RNase. A notoriously stable...

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