Báo cáo khoa học: Contribution of a central proline in model amphipathic a-helical peptides to self-association, interaction with phospholipids, and antimicrobial mode of action ppt

Báo cáo khoa học: Contribution of exofacial thiol groups in the reducing activity of Lactococcus lactis docx

... Eh7was linked to anincrease in exofacial thiol groups (Fig. 5A, phase 1) and the Eh7ceased to decrease and remained stablewhen the maximal amount of thiol groups was reached(Fig. 5A, phase ... (Axioplan 2i; Carl Zeiss,Jena, Germany). Images were acquired using axiovision4.8 software and an AxioCam MRm digital camera(Carl Zeiss).Protein extraction and blotting analysisBacterial ... CCCPstock solution was prepared in methanol with a ﬁnal CCCPbatch concentration of 152 lm. A nigericin : valinomycinstock solution was prepared in methanol with a concentra-tion of 1.6 and...

Báo cáo khoa học: Contribution of a central proline in model amphipathic a-helical peptides to self-association, interaction with phospholipids, and antimicrobial mode of action ppt

... of a central Pro in membrane-active peptides. To determine the role of Pro in structure,antibiotic activity, and interaction with phospholipids, we generated a ser-ies of model amphipathic a- helical ... liposomes. In addition, biosensortechnology indicated that the interaction of PCPs with membranes was predominantly in uenced by initialelectrostatic interactions, whereas the interaction of PFPs with ... Taneja AK & Hodges RS (1984) Synthesis of a model protein of deﬁned secondary and quaternarystructure. Effect of chain length on the stabilization and formation of two-stranded alpha-helical...

Tài liệu Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses pdf

... Gagne´M & Rappe´AK (1998)p-Stacking interactions. Alive and well in proteins.J Biol Chem 273, 15458–15463.29 Kashiwagi T, Jeanteur D, Haruki M, Katayanagi M,Kanaya S & Morikawa ... extension: tailor-made genes using thepolymerase chain reaction. Biotechniques 8, 528–535.34 Kanaya S, Katsuda C, Kimura S, Nakai T, Kitakuni E,Nakamura H, Katayanagi K, Morikawa K & IkeharaM ... Koga1, Kazufumi Takano1,3 and Shigenori Kanaya11 Department of Material and Life Science, Graduate School of Engineering, Osaka University, Japan2 Department of Applied Chemistry, Graduate School...

Tài liệu Báo cáo khoa học: "TREATMENT OF LONG DISTANCE DEPENDENCIES IN LFG AND TAG: FUNCTIONAL UNCERTAINTY IN LFG IS A COROLLARY IN TAG" ppt

... functional uncertainty, as defined by Kaplan and Zaenan [3] and Kaplan and Maxwell [2]. In this paper, we relate this characterization to that provided by Tree ~,djoining Grammars (TAG), showing ... level). In [10], we have formal- ized this notion by introducing graph adjoining grammars which generate exactly the same lan- guages as TAGs. In a graph adjoining grammar, /~x is represented as ... elementary trees in a TAG provide an extended domain of locality, and factor out recursion and dependencies. A computational consequence of this result is that we can obtain these analyses without...

Báo cáo khoa học: Prediction of missing enzyme genes in a bacterial metabolic network Reconstruction of the lysine-degradation pathway ofPseudomonas aeruginosa doc

... 5’-GGAATTCCATATGCAACTCAAAGATGCCAAGCTG)3’ and 5’-CCCAAGCTTGATACCGCCCAGGCAGAGGTACTTG-3’.The primers used for the PCR cloning of PA0266 wereas follows: 5’-GGAATTCCATATGAGCAAGACCAACGAATCCC-3’ and 5’-CCGCTCGAGAGCGAGTTCGTCGAAGCACTCGG-3’. ... Schematic drawing of reactionscatalyzed by aminovalerate aminotrans-ferase (PA0266) and glutarate semialdehydedehydrogenase (PA0265). (B) Activity of PA0265 and PA0266. The reaction was car-ried ... pathway in P. aeruginosa.We ﬁlled in some of the enzyme genes in the lysine-degradation pathway, for example, by predictingPA0266 as a putative 5-aminovalerate aminotrans-ferase and PA0265 as...

Báo cáo khoa học: Reconstruction of the central carbon metabolism of Aspergillus niger pot

... investigation in the literature and reaction or pathway databases.Some enzymes may catalyse several reactions, anexample being transketolase (EC 2.2.1.1) in the pentosephosphate pathway that catalyses ... speciality chemicals and pharmaceuticals. Moreover, they play a major role forhuman welfare as agents of biodegradation, spoilage and decay, and some ﬁlamentous fungi act as pathogens forhumans, ... and here the availability of experimental data plays an importantrole. Once validated, the model can be used as a tool for theanalysis, interpretation and prediction of metabolic beha-viour and hence...

Báo cáo khoa học: Dynamics of flavin semiquinone protolysis in L-a-hydroxyacid-oxidizing flavoenzymes – a study using nanosecond laser flash photolysis doc

... limited to a narrow time window because of rapid pH relaxation to the initial state after the pH jump.We have instead made use of the transformation of a photoreactive species into a stable product ... runs at 2 and 5 mm EDTAgave the same deprotonation rates. Thus, a reaction with EDTA can be neglected, and so can a reaction with OH)at the pH values used here. This leaves Tris and H2O as ... individual measurements but arerepresentative of a number of experiments.Table 1. Rate parameters of FMNH•deprotonation. Rate constants of FMNH•deprotonation in the FDH domain and in LCHAO in 50...

Báo cáo khoa học: Prediction of coenzyme speciﬁcity in dehydrogenases ⁄ reductases A hidden Markov model-based method and its application on complete genomes doc

... belong to the folds ‘NAD(P)-binding Rossmann-fold domain’, ‘FAD ⁄ NAD(P)-bindingdomain’ and ‘Nucleotide-binding domain’. The dataset wasscrutinized and only proteins utilizing FAD or NAD(P) in a ... iter-atively training the two HMMs, one with FAD-bindingsequences and one with NAD(P)-binding sequences, usingsequences from five of the groups and testing against theremaining group and a false data set. ... classified asNADP-binding. The group with most failures is NADP-binding proteins, with a total of 13 false negatives: eightare lost during database search, three are classified as false,and...

Báo cáo khoa học: Presence of melanocortin (MC4) receptor in spiny dogﬁsh suggests an ancient vertebrate origin of central melanocortin system pot

... receptor has a role in pigmentation and it also mediates the anti -in ammatory action of MSH [11].Our understanding of the mechanisms of appetite regu-lation and metabolism in mammals is increasing ... and counted with Wallac 1450 (Wizard automatic Microbetacounter). The results were analyzed with a softwarepackage suitable for radioligand binding data analysis(PRISM3.0, Graphpad Software, San ... CCTCTT GGA AAA TAT TTT GGT CAT TG and 3¢-GACCAA AAT ATT TTC CAA GAG GCT GAA GAT G.The primers were hybridized to opposite strands of thereceptor gene and the complete coding sequence wasampliﬁed....

Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx

... 10.106 Takaki E, Fujimoto M, Sugahara K, Nakahari T,Yonemura S, Tanaka Y, Hayashida N, Inouye S,Takemoto T, Yamashita H et al. (2006) Maintenance of olfactory neurogenesis requires HSF1, a major ... The Authors Journal compilation ª 2010 FEBS in mammals, the crystallins are aA and aB; bB1, bB2,bB3, bA3 ⁄ A1 and bA4; and cA, cB, cC, cD, cE, cF and cS. In mice, the c-crystallins are the major ... transcriptionfactor 1 deﬁciency alters cardiac redox homeostasis and increases mitochondrial oxidative damage. EMBOJ 21, 5164–5172.36 Yan LJ, Rajasekaran NS, Sathyanarayanan S & Benja-min IJ (2005)...

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