Báo cáo khoa học: Deoxyribonuclease I footprinting reveals different DNA binding modes of bifunctional platinum complexes potx

... mole-cules of biological significance [1,6–15], includingantitumor cis-diamminedichloroplatinum(II) (cisplatin)(Fig. 1B) and its clinically ineffective trans isomer [16].The bifunctional platinum complexes, ... high sensitivity of DNase I to DNA conformational alterations induced by platinum com-plexes so that the platinum adducts which induce specific local conforma-tional alterations in DNA are differently ... formation, identification, and quantitation.Biochemistry 24, 707–713.32 Anin MF & Leng M (1990) Distortions induced indouble-stranded oligonucleotides by the binding of cis-diamminedichloroplatinum(II)...

Tài liệu Báo cáo khoa học: Molecular cloning, recombinant expression and IgE-binding epitope of x-5 gliadin, a major allergen in wheat-dependent exercise-induced anaphylaxis ppt

... recombinant x-5 gliadin protein thatcan be used for diagnosis and possibly treatment of WDEIA.ResultsIdentification of IgE -binding epitopes inx-5 gliadinThe IgE -binding epitopes of x-5 gliadin ... patients.The binding of IgE to nOG5 was completely inhibitedby increasing amounts of rOG5C in all patients. At aninhibitor concentration of 0.1 and 1 lgÆmL)1, nOG5inhibited IgE binding ... effectively than rOG5C(Fig. 5).DiscussionIn this study we identified new linear IgE -binding epi-topes in x-5 gliadin and described the gene cloning,expression in E. coli, purification, and immunologicalcharacterization...

Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx

... thatfolding of G-rich oligonucleotides into quadruplexstructures proceeds via kinetically signiﬁcant interme-diates. These intermediates may consist of antiparalleldouble hairpins in rapid equilibrium ... Coupling of localfolding to site-speciﬁc binding of proteins to DNA. Science 263, 777–784.50 Lane AN & Jenkins TC (2000) Thermodynamics of nucleic acids and their interactions with ligands. ... concentrations of any intermediate spe-cies. Calorimetry (differential scanning calorimetry,DSC, and isothermal titration calorimetry) can be usedto monitor quadruplex folding and unfolding reac-tions,...

Báo cáo khoa học: Structural and thermodynamic insights into the binding mode of ﬁve novel inhibitors of lumazine synthase from Mycobacterium tuberculosis pptx

... thearomatic pyrimidine ring and the indole group of Trp27 should to be weaker in comparison with thepurinetrione inhibitors due to the smaller size of thepyrimidine ring. It has in addition resulted ... activesite with an inhibitor molecule of proper size, shapeand atomic composition clearly stabilizes the interac-tion of the subunits within the pentamer. This conclu-sion is in line with ... (DG) of the binding reactions. Figure 7 showsrepresentative calorimetric titration curves of MbtLSwith different inhibitors. Earlier crystallographic stud-ies of lumazine synthases from various...

Báo cáo khoa học: D-Amino acids in the brain: the biochemistry of brain serine racemase potx

... wasestablished with its N-terminal 66 residues. Binding of Golga3 increases d-serine synthesis. Intriguingly, this isachieved through a decrease in ubiquitin ⁄ proteasomaldegradation of serine racemase, ... of calcium, it is possible that some otherGRIP-interacting protein that also binds to PDZ6might disrupt the serine racemase–GRIP interaction,hence diminishing the activity of the former.In ... phosphorylation. Thus,d-serine production in the brain is tightly regulated by various factorspointing at its physiologic importance. In this minireview, we will focus onthe regulation of brain serine...

Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf

... its 72 kDa progelatinase complex.Demonstration of the biochemical similarities of tissueinhibitor of metalloproteinases-2 and tissue inhibitor of metalloproteinases-1. Biochem J 278, 179–187.16 ... cysteine-rich protein with kazal motifs (RECK) is a cell mem-brane associated inhibitor of MMP-2 that is able toinhibit the second activation step of MMP-2 [9].The small calcium -binding protein ... metastasis process, depending on the time andlocalization of their expression. The N-terminal part of TIMPs is involved in binding to the active site of MMPs and hence prevents their action, whereas...

Tài liệu Báo cáo khoa học: Type I antifreeze proteins expressed in snailﬁsh skin are identical to their plasma counterparts doc

... all fishexamined had significant levels of protein in blood andskin during the winter. It is possible that different phy-siological or environmental cues initiate expression ineach tissue separately. ... as important for ice binding [21–23]. Recently,amino acid substitution experiments have determinedthat it is the conserved Ala-rich hydrophobic surfacewhich is most important for ice -binding ... from liver of Atlantic anddusky snailfish using a WizardÒ Genomic DNA PurificationKit (Promega, Madison, WI, USA). Aliquots of RNAseA-treated genomic DNA were digested with various restric-tion...

Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-speciﬁc intronic inverted repeats pptx

... expression. Thestem-loops and palindromes constructed by invertedrepeats are involved in RNA interference, transcriptioninitiation of genes, initiation of DNA replication andalternative splicing ... cruciforms: significance for initiation of DNA replication. J Cell Biochem 63, 1–22.25 McAlinden A, Havlioglu N, Liang L, Davies SR &Sandell LJ (2005) Alternative splicing of type II procol-lagen ... comprise one line of evi-dence for the functional role and adaptive evolution of the primate LIRs.The biological profiling of the orthologs withhuman-specific LIRs implies their association with thedevelopment...

Báo cáo khoa học: Transcript proﬁling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice pdf

... gene families in rice. In this study, whole genome microarrayanalysis of auxin-responsive genes in rice was performed, with the aim of gaining some insight into the mechanism of auxin action. A ... positively regulatethe expression of downstream genes involved in auxinsignaling. The differential and overlapping expressionpatterns of individual members of these gene familiesin rice offer ... analysis reveals similarities and variations in genetic programscontrolling pollination ⁄ fertilization and stressresponses in rice (Oryza sativa L.). Plant Mol Biol 59,151–164.63 Jain M, Nijhawan...

Báo cáo khoa học: Modular kinetic analysis reveals differences in Cd2+ and Cu2+ ion-induced impairment of oxidative phosphorylation in liver pot

... compilation ª 2009 FEBSMetal-induced impairment of mitochondrial function J. Ciapaite et al.Modular kinetic analysis reveals differences in Cd2+andCu2+ion-induced impairment of oxidative ... quantiﬁcation of microgram quantities of proteinutilizing the principle of protein–dye binding. AnalBiochem 72, 248–254.40 Ciapaite J, Van Eikenhorst G, Bakker SJ, Diamant M,Heine RJ, Wagner ... peroxidation of membrane lipids.Modular kinetic analysis uses natural properties of metabolism, i. e. its organization into recognizable func-tional units, simplifying the analysis and making cellularcomplexity...

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