Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx

... here the characterization of the ﬁrst mammalian-like purine nucleoside phosphorylase from the hyperthermophilic archaeon Pyrococcus furiosus (PfPNP). The gene PF0853 encoding PfPNP was cloned and expressed ... Puriﬁcation and characterization of extremely thermophilic and thermostable 5 ¢-methylthio-adenosine phosphorylase from the archaeon Sulfolobussolfataricus. Purine nucleodide phosphorylase activity and ... 5¢-deoxy-5¢-methylthioadenosine phosphorylase; PfMTAP, 5¢-deoxy-5¢-methylthioadenosine phosphorylase from Pyrococcus furiosus; PfPNP, purine nucleoside phosphorylase from P. furiosus; PNP, purine nucleoside phosphorylase; ...

Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf

... products of the hazelnutLOX were compared with the reaction products of soybeanLOX type I and authentic standards of 9- and 13-HODE.As shown in Fig. 4, 9-HODE is the main product of the hazelnut ... providing the substrates for the activity of all the enzymes located downstream on the pathway, have a primary role in the biosynthesis of phytooxylipins and can affect their availability inside the plant ... aim to betterclarify the occurrence of LOXs and their inﬂuence onhazelnut seed quality, we carried out the biochemical and molecular characterization of the main LOX isoformsexpressed during...

Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... only one band wasampliﬁed from each of the different samples and Southern blot analysis using the probe prepared with the lac1 primers also revealed a single band. Further-more, all of these generated ... to the culture medium [1]. In this study, we havepuriﬁed and characterized one of the laccase isoforms,cloned and sequenced the cDNA encoding the enzymeprotein, and examined the effect of ... positively affected the activity and stability of the enzyme [40]. The effect of copper onenzyme stability may be related to the inhibitory effect of the metal on the activity of an extracellular...

Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deﬁcient families from Southern Italy docx

... Erlandsen and Stevens [22]. The details of the interactions displayed by residues in the neighborhood of Q301 were analyzed in the structure of the ternary complex of human PAH with BH4 and ... damage and mental retardation and epilepsy, as well as otherneurological abnormalities [3]. The severity of PAHdeﬁciency is variable and partly depends on the nature of the mutations of the PAH ... (Fig. 1A), in the presence of anti-PAH serum, the intensity of the band correspondingto the 50 kDa monomeric form of the mutant enzymeTable 1. Distribution of mutations along the PAH gene ⁄...

Báo cáo khoa học: Biochemical and spectroscopic characterization of the bacterial phytochrome of Pseudomonas aeruginosa pdf

... describe the further biochemical and spect-roscopic characterization of BphP.ResultsExpression, puriﬁcation and initial characterization of recombinant P. aeruginosa phytochrome The P. aeruginosa ... almost all other known members of the classicphytochrome family [12,24]. More recent reports alsorevealed the presence of the Pfr ground state in Agp2 from A. tumefaciens and the BphPs of Rhodopseudo-monas ... spectral shift of the Pr and Pfrforms. Therefore, the BLD may play a role in stabil-ization and co-ordination of the chromophore and possibly its covalent attachment to Cys12 (i.e. the bilinlyase...

Báo cáo khoa học: Synthesis and structural characterization of a mimetic membrane-anchored prion protein doc

... Research, Aliso Viejo, CA) and wasfound to be similar to the pore size of the membrane usedfor the extrusion process. The change in the size and poly-dispersity of the liposomes was minimal ... [15]. In this study the protein is nativelyfolded and retains the two glycosyl moieties but iscleaved from the GPI anchor and therefore released from the membrane surface.There is no high-resolution ... thor-ough understanding of the folding and interactions of PrP in its tethered conformation on the plasma mem-brane. The interaction of PrP with different lipid compo-nents is complex and is not...

Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx

... provides the proton motive force essential for the synthesis of ATP. Homologs of this complex existin bacteria, the mitochondria of eukaryotes, and the chloroplasts of plants. The bacterial and mitochondrialKeywordschloroplast; ... electrophoresisfor the separation of proteins in the range from 1 to100 kDa. Anal Biochem 166, 368–379.42 Laemmli UK (1970) Cleavage of structural proteinsduring the assembly of the head of bacteriophageT4.Nature ... transcription of the ndh genes is much higher in BS chloroplasts, and elevated amounts of the Ndh complex have been foundin these plastids [18]. The function of the Ndh complex is still a matter of debate....

Báo cáo khoa học: Biochemical and molecular characterization of puriﬁed chicken pancreatic phospholipase A2 docx

... of the hydrolysis products [12,19]. In order to investigate the effect of bile salts on ChPLA2activity, the rate of hydrolysis of PC by ChPLA2in the presence of vari-ous concentrations of ... mass of 14 kDa and aspecific activity of 400 UÆmg)1in the presence of 1 mm sodium taur-odeoxycholate and 4 mm CaCl2with phosphatidylcholine as substrate. The N-terminal sequence of the first ... [15] biochemicallycharacterized an active thermostable PLA2 from ostrich pancreas (OPLA2). It is therefore interesting toinvestigate more biochemical and structural properties of other purified...

Báo cáo khoa học: Biochemical and structural analyses of a higher plant photosystem II supercomplex of a photosystem I-less mutant of barley pot

... bands of the WT and viridis zb63. The quantification of the bandsin the two gradients is presented normalizedto the photosystem II (PSII) reaction centre(RC) core band. (C) SDS profile of bands ... solubilization of the viridis zb63 thylakoidmembranes and the analyses of one of the resultingsucrose density fractions (band 7) confirmed the pres-ence of these LHCII–PSII supercomplexes in the thyl-akoids ... prevents the formation of extensive arrays of PSIIcomplexes of the type observed in the grana of viridiszb63.A further factor in the stability of PSII arrays in vir-idis zb63 could be the presence...

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... A3(2).Cloning and expression of the putative hydgenes The deduced open reading frames of putative hydgenes were PCR-ampliﬁed from the genomic DNA of the corresponding organisms. The PCR fragments of the ... performed usingT-COFFEE [44].Docking simulation of substrate on the active site of HYDJs The substrate binding pockets of HYDJs and the mode of interaction between substrate and enzyme were ... for the catalytic activity, even though there may be otherrequirements for residues at other positions, such asside-chain size or polarity. Better understanding of the roles of each of these...

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