... mechanism of cofactor binding as
Fig. 6. Progress curves of the reconstitution of EcIPDC with ThDP
measured by restoration of the catalytic activity of the formed holo-
enzyme for the substrate ... indolepyruvate (Fig. 1). In
the course of the establishment of the equilibrium % 85% of
the enol form is converted into the ketone (half-time
% 8 min at 20 °C) a...
... bilayers, and their
dependence on calcium. Therefore, the objectives of
this study were to analyze (a) the effect of KL
4
on the
physical properties of membranes, in the absence and
presence of Ca
2+
, ... probably
involved the extraction of PA from the bilayer at con-
centrations of > 100 lm calcium. Thus, the formation
of PA–Ca
2+
complexes might explain the...
... with the
higher inhibitory activity of the cEL peptide and the very
low inhibitory activity of the cYT peptide compared to
other peptides.
The flanking residue of the b-turn, i.e. Tyr3 of the cVY
peptide ... suggestive of a b-turn in the peptide and
the proximity of amide protons due to compact nature of
the structure. However, t he coupling constant of...
... between the two isoforms of the protein,
only the molecular mass of 50 kDa would suggest that we
had purified the truncated isoform.
Cloning of the cDNA of two isoforms of tomato
b-fructofuranosidase ... patients.
Hence, we concluded that the IgE reactivity of the natural
protein mainly depends on the glycan structure. The amino
acid sequences of both isofor...
... assay
The LAL test is based on the property of lysates of
amebocytes of the horseshoe crab Limulus polyphemus,to
form a solid gel in the presence of minute amounts of
endotoxins. The comparison of ... answer the question
whether the cubic structure observed is of the ÔnormalÕ
(right side out) or the inverted type, the geometry of the
molecule with its bul...
... positioning of
the aromatic ring of Tyr456 in the closed state. In the
absence of a closed complex of E542K P2Ox, the effect
of the loss of this hydrogen bond on the packing of
the substrate loop is difficult ... those of the native
proteins. The second set of spectra were recorded after partial
thermal unfolding, whereas the final spectra show the loss...
... as a result of the decrease in pH was
assayed by measuring the radioactivity of tritium, as described in
Materials and methods (In the figure, the y-axis represents the
cpm values of pellet). ... Ca
2+
channel in the ER, which
increases Ca
2+
leakage via a mechanism dependent on
both the pH and the C-terminal cytosolic region of the
protein [9]. However, the preci...
... extent of
ATPase activity at NBD1 and NBD2 remains the
same in full-length SUR1 (i.e. that of NBD1 is
20% of that of NBD2), then the marked inhibition
of ATPase activity of SUR1 suggests that the ... filtration,
especially in the case of SUR1F, diluted the amount of
protein available and was therefore not used routinely.
However, no difference in the...
... interruption of the geometry
of the recognition helix for either Antp or the TTF-1
HD, the anomalous amide exchange pattern and the
NOE connectivity data of the C-terminal portion of
helix III ... inertia.
In the context of RSDM, the detailed analysis of the
three spectral densities J(0), J(x
N
) and J(0.87x
H
)
allowed us to obtain a rather complete description...
... one
to develop a broader perspective of the questions
within an analysis of the hypothesis in the con-
text of a characterization of the "how" of the
entire behavior.
By adapting ... a result of acti-
vation of a pragmatic representation of a
disambiguated word meaning.
It is the in interaction of the results of
these asynchronous processes...