Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc

... speci-ﬁc roles of the various members of the DIM family,e.g. DIM A and DIM B, in these functions have neverbeen investigated. The recent developments in ourunderstanding of the biosynthetic ... between the number of CFUs of the wild-type strain and that of the PMM56 mutantstrain in lungs and in the spleen (Fig. 7A). Indeed,both strains multiplied in lungs, but whereas an aver-age of 5.92 ... biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis Roxane Sime´one, Patricia...

Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... base-pairs in the centre of the junctions [19]. Detailedunderstanding of the functional role of these interactionswould require the crystal structure of the Ydc2–junctioncomplex. In this study the ... [14,20]. Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs[19], which is reminiscent of the structure ... conformation of the junction, then the deletion of this motif would have aneven more serious effect on Ydc2 binding in the presence of divalent cations. We tested the effect of Mg2+on the binding of the...

Báo cáo khoa học: Molecular mechanisms of the phospho-dependent prolyl cis ⁄ trans isomerase Pin1 docx

... least for the Fig. 2. Schematic view of the parallel between Pin1 and CKS in protein degrada-tion. Top: Model of the SCFCDC4E3 ligase and the role of Pin1. Pin1 is brought in with the cyclin E ... similar bindingpockets of Pin1 and FKBP and the structural resem-blance between their respective high-afﬁnity inhibitors[28] further underscore the similarity between Pin1 and the other peptide ... novel inter-action surfaces for two distinct molecular partners [6], and the conformation of a proline in the linker region between two SH3 domains of the Crp adaptor proteindetermines the autoinhibition...

Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identiﬁes a calcium sensitive region required for basal activity doc

... weresubcloned into pBSSK (Stratagene). The structure of the gene including the 5¢ and 3¢ ﬂanking regions was determinedby double-stranded DNA sequencing, exons were identiﬁedaccording to the sequence of ... sites within the MGP coding sequence(Fig. 2 ). Analysis of the phase of each of the xMGP intronslocated within the coding region revealed that introns 2 and 3 are of phase I while intron 4 is ... UTR(260)Fig. 2. Sites of intron insertions within the amino-acid sequence of Xenopus, human and mouse MGPs. Conserved sites of intron insertions in mammalian and X. laevis MGPs are boxed. The gamma-carboxyglutamate...

Báo cáo khoa học: Molecular basis of the unusual catalytic preference for GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase doc

... structures of the wild-type, single- and double-mutant EhPGKs in aclosed conformation will certainly help to clarify the molecular arrangement in the guanine ring binding siteduring the binding of ... B); and Val339, Gly340 and Val341 (patch C). The corresponding reported amino acid residuesinvolved in adenine binding in the other crystallizedproteins, as well as in the EhPGK identiﬁed in the predicted ... carbon 2 of the guanine ring may interact with the side chain of Glu309, whereas the carbonyl group atposition 6 of the guanine ring may interact with the hydroxyl group of the Tyr239 side chain (Figs...

Báo cáo khoa học: Molecular dynamics of the DNA-binding domain of the papillomavirus E2 transcriptional regulator uncover differential properties for DNA target accommodation pdf

... gain insights into the structural dynamics of the DNA-bindingdomains of two prototypic strains, human papillomavirus strain 16 and the bovine papillomavirus strain 1. The simulations underline ... toalanine of the residues Lys325 and Lys327, located in the b2–b3 loop, produce a decrease in the DNA bind-ing [22] that is restored upon back mutation intoarginine, indicating the importance of ... (A), the black dotted line represents the S2NMR values; in (A) and (B), the black and the gray point-dashedlines show the values calculated in MD for chain A and chain B,respectively. The...

Báo cáo khoa học: Molecular cloning of the ecdysone receptor and the retinoid X receptor from the scorpion Liocheles australasiae pot

... B(transactivation domain), C (DNA-binding domain),D (hinge region) and E ⁄ F (ligand- or hormone-bindingdomain) [8,9]. The X-ray crystal structures of the ligand-binding domains of EcR, USPs and RXRs havebeen ... speciﬁc binding of LaEcR-A was not increased in the presence of LaRXR. These results are in contrast to the insect receptors where the speciﬁc binding of PonAto EcR was markedly increased in the ... subdivided into two subphyla – the Mandibulata and the Chelicerata; the former includes the classes Insecta and Crustacea; and the latterincludes the class Arachnida, which contains the scor-pions,...

Báo cáo khoa học: Molecular analysis of the interaction between cardosin A and phospholipase Da Identiﬁcation of RGD/KGE sequences as binding motifs for C2 domains pdf

... [12,14,15,19,20,22],there are no reports on the true nature of the RGD-containing proteins and their interacting partners. The involvement of the PLDa C2 domain in theseFig. 7. C2 domain is degraded by cardosin ... at the tip of a loop protruding away from the core of the protein. This amino-acid motif mimics RGD in terms of charge and is positioned at the tip of a loop and istherefore reminiscent of RGD ... at the surface of the protein [4]. The presence of this well-known integrin-binding motif [11], and the previouspuriﬁcation of a cardosin A-binding protein from pol-len, raised the idea that...

Báo cáo khoa học: Structural ﬂexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for speciﬁc substrate recognition pptx

... linked, and incorrectbinding of ATP can affect serine binding and viceversa [22]. Asn435, together with Asp416 and Glu432,contributes to binding of the Mg2+ion between the a- and b-phosphates of ... r.m.s.d of Caatoms after superposition of the catalytic domains in complex with different substrates and the cata-lytic domain of the apo-enzyme. The binding of the small substrates in the active ... of the conserved arginine residues in the motif 2 loop show aconcerted movement following substrate binding in the active site. In the apo-enzyme, the motif 2 Arg336occupies the adenine-binding...

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... strainsrevealed the presence of a single point mutation in 1337 nucleotides of BmIVD. In the sku mutant, the 1127th guanine from the start codon was substitutedby thymine and none of the other sites ... acts in the third step of leucine degradation and the sku mutant accumulates branched-chain amino acids in haemolymph, thismutant may be useful in the investigation of unique branched-chain aminoacid ... sku and BmIVD genesTo determine the consistency between the sku pheno-type and the BmIVD genotype, linkage analysis between the wild-type and sku strains was performed.For this, crossing of the...

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