Báo cáo khoa học: Crystal structure of a cold-adapted class C b-lactamase potx

Tài liệu Báo cáo khoa học: Crystal structure of a subtilisin-like serine proteinase from a psychrotrophic Vibrio species reveals structural aspects of cold adaptation docx

... I, Moe E, Lanes O, Smalas AO & Willassen NP(2003) The structure of uracil–DNA glycosylase fromAtlantic cod (Gadus morhua) reveals cold-adaptationfeatures. Acta Crystallogr Sect D 59, 1357–1365.10 ... CA, USA].AcknowledgementsWe thank Andrea Schmidt for assistance during datacollection at DESY beamline BW 7A and Achim Dick-manns for comments on the manuscript.References1 Blochl E, Rachel ... structure of proteinase K (PDB accession number, 1IC6) as a search model. The crystallized 30 kDa catalytic domain of Vibrio proteinase encompasses amino acids 140–420 of the 530 amino acid prepro-enzyme...

Báo cáo khoa học: Crystal structure of a glycoside hydrolase family 6 enzyme, CcCel6C, a cellulase constitutively produced by Coprinopsis cinerea pot

... A ˚(CcCel 6C HinCel 6A, 1BVW), 1.14 A ˚(CcCel 6C HjeCel 6A, 1QK0 chain A) and 1.30 A ˚(CcCel 6C HinCel6B, 1DYS chain A) formain chain atoms.The signiﬁcant feature in cellobiohydrolasesHjeCel 6A and ... areillustrated as stick models. (C) Comparison of the Ca backbones of unliganded CcCel 6C (blue), CcCel 6C pNPG3 (green) andCcCel 6C cellobiose (red).Y. Liu et al. Structure of C. cinerea CcCel 6C FEBS ... CcCel 6C (Fig. 2)indicated that Asp150 and Asp334 of CcCel 6C arethe potential catalytic residues and could act as a proton donor and a base, respectively. Another aspar-tic acid residue (Asp175 of...

Báo cáo khoa học: Crystal structure of a cold-adapted class C b-lactamase potx

... transpeptidase. b-Lactamases of Keywords class C b-lactamase; cold adaptation;psychrophile; weak interactions; X-ray structure Correspondence C. Michaux, Chimie Biologique StructuraleLaboratory, CPTS ... the structure of the class C b-lactamase from E. cloacae P99 (Protein DataBank code 2BLT) as a search model. The model wassolved to a resolution of 2.2 A ˚. A summary of datacollection and reﬁnement ... (ENTCL) and S. mar-cescens (SERMA). The three motifs characteristic of active site serine b-lactamases are in red. The disordered sequences of PSEFL are ingreen.Psychrophilic class C b-lactamase...

Báo cáo khoa học: Crystal structure of a staphylokinase variant A model for reduced antigenicity pptx

... using a- cyano-4-hydroxycinnamic acid (CCA) as the matrix.Protein crystallizationCrystallization trials were carried out using the hanging-drop vapor-diffusion method at 293 K. Crystals wereobtained a few days after ... dimerinterface. The total buried surface areas of the a a or head±tail geometries were more than 1000 A Ê2, m uch larger thanthe a verage buried surface area of random crystal packing[31]. ... Beijing, China;2Department of Molecular Genetics, Shanghai Medical University, ChinaStaphylokinase (SAK) is a 15.5-kDa protein from Staphy-lococcus aureus that activates plasminogen by forming a...

Báo cáo khoa học: Crystal structure of a designed tetratricopeptide repeat module in complex with its peptide ligand pot

... com-posed of three TPR repeats (AB-helix pair) and anadditional C- terminal capping helix (A cap). The onlyway for molecules AB–AB–AB A capto arrange on‘head-to-tail’ packing is if the C- terminal ... Hsp90-speci c residues ‘grafted’ onto the bindingsurface of CTPR3 [5]. The pairwise backbone align-ment of CTPR3 (Protein Data Bank ID: 1Na0) and A BFig. 2. Crystal packing of CTPR390. (A) R3 crystal ... the‘additionally allowed’ regions of the Ramachandranplot. Crystal packing – head-to-tail packingThe parent protein, CTPR3, crystallized as a monomerwith two molecules in the ASU. It was therefore...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... digital abstractlCLIC4 and importin alpha bind by x-ray crystallography (View interaction)AbbreviationsARM, armadillo; CLIC, chloride intracellular channel; NLS, nuclear localization signal; ... integration of macromoleculardiffraction data. Acta Crystallogr D: Biol Crystallogr62, 48–57.36 Collaborative Computational Project Number 4 (1994)The CCP4 suite: programs for protein crystallography.Acta ... (CLIC)protein, CLIC4, translocates to the nucleus in response to cellular stress,facilitated by a putative CLIC4 nuclear localization signal (NLS). TheCLIC4 NLS adopts an a- helical structure in the native...

Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

... Holst MJ & McCammonJA (2001) Electrostatics of nanosystems: Applicationsto microtubules and the ribosome. Proc Natl Acad SciUSA 98, 10037–10041.39 Wallace AC, Laskowski RA & Thornton ... 6-phytases.However, the crystal structure of E. coli AppA showsphytate bound with the 3-phosphate as a scissilegroup, although its biochemical characterization classi-ﬁes it as a 6-phytase. ... RHGXRXP and HD, andhydrolyze metal-free phytate with pH optima in theacidic range. They consist of two domains, a large a ⁄ bdomain and a small a domain with the catalytic site atthe interface of...

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... expression analysis betweensquamous cell carcinoma of the head and neck andnormal tissue led to the identiﬁcation of a differentialexpressed squamous cell carcinoma gene 1 (DESC1).The data indicated ... precursor by human colorectal carcinomacells and its modulation by cytokines ⁄ growth factorsand proteinase inhibitors. Biol Chem 380, 473–483.17 Kataoka H, Uchino H, Asada Y, Hatakeyama K,Nabeshima ... a new software suite for macromolecular struc-ture determination. Acta Crystallogr D Biol Crystallogr54, 905–921.29 Engh RA & Huber R (1991) Accurate bond and angleparameters for X-Ray...

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... hyper-thermophilic archaeon Thermococcus kodakaraensisKOD1. J Bacteriol 185, 5175–5180.14 Tanaka T, Fukui T, Fujiwara S, Atomi H & ImanakaT (2004) Concerted action of diacetylchitobiose deacety-lase and ... fromTable 1. Deacetylase activity of the BcZBP protein on N-acetylchi-tooligosaccharides.Substrate Deacetylation (%)GlcNAc 100(GlcNAc)289.82(GlcNAc)310.51(GlcNAc)45.51(GlcNAc)512.19(GlcNAc)63.93Fig. ... cereus is an opportunistic pathogenic bacterium closely related toBacillus anthracis, the causative agent of anthrax in mammals. A signiﬁcantportion of the B. cereus chromosomal genes are common...

Báo cáo khoa học: Crystal structure of basic 7S globulin, a xyloglucanspeciﬁc endo-b-1,4-glucanase inhibitor protein-like protein from soybean lacking inhibitory activity against endo-b-glucanase doc

... DASA of the AB dimer isdeﬁned as [(ASA of A) + (ASA of B) ) (ASA of AB dimer)] ⁄ 2. Thenumber of water molecules in the dimer interface was detectedwithASV CALCULATOR [34]. ASA was calculated ... Kinoshita S, Tomatsuri H, UmedaK, Ueda S, Hata Y & Arai M (1999) Analysis of a cat-alytic acidic pair in the active center of cellulase fromAspergillus aculeatus. Biosci Biotechnol Biochem 63,2157–2162.33 ... Yokohama City University, Japan2 Graduate School of Pharmaceutical Science, The University of Tokyo, Japan3 SAITO Research Center, Peptide Institute Inc., Osaka, Japan4 Graduate School of Science,...

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