Báo cáo khoa học: Something from nothing ) bridging the gap between constraint-based and kinetic modelling pot
Báo cáo khoa học: Something from nothing ) bridging the gap between constraint-based and kinetic modelling pot
... 1ETOH ) 2.95 ) 1NAD ) 3.04 ) 1NADH 3.20 1ALD F16P 1.89 1TRIO ) 3.08 ) 2ATP P 1.80 1ENO P2G 0.826 1PEP ) 0.384 ) 1GAPDH BPG ) 8.00 · 10 )2 ) 1NAD 0.144 1NADH ) 9.14 · 10 )2 ) 1TRIO ... Michaelis–Menten kinetics v(x) ¼ Vx⁄(x + Km ) (o) to its linlog approximation u(x) ¼ v*(1+e log(x ⁄ x *)) (solid line) and its power law approximation x(x) ¼ v*(x ⁄ x *) e(dashed line), where v* ¼ v(x *) and ... 1P ) 9.47 ) 1P3G ) 2.24 ) 1PGM P2G ) 2.36 ) 1P3G 2.94 1PYK P ) 1.82 ) 1PEP 0.765 1PYR ) 0.243 ) 1K. Smallbone et al. Constraint-based meets kinetic modelling FEBS Journal 274 (200 7) 5576–5585...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
... splicing (Fig. 6B) and therefore it seemsreasonable to make the working hypothesis that the RNA and DNA binding sites are not the same, rather that theyare separate. This does not exclude the possibility ... toresult from slow release of the cleavage products from the protein. The amplitudes of the initial burst (2.78 and 2.83 nM ) gave a ratio of DNA/protein of 0.94 : 1. As will bediscussed further, these ... (data not shown). The rate constant, kon,for the binding of RNA to protein is equal to 3 · 109M )1 Æmin )1 under similar conditions [38] to these. We measured the rateconstant for the binding...
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Báo cáo khoa học: High molecular weight kininogen binds to laminin – characterization and kinetic analysis pot
... plasma) were then added to wells coatedovernight with LM (10 lgÆmL )1 ), VN (5 lgÆmL )1 ) and FN(10 lgÆmL )1 ), respectively, and blocked with Locke’s buffer contain-ing 1% (w ⁄ v) BSA. (B) Alternatively, ... Purified HK and HKa Ligand coated on the microtiter plateFig. 1. Extraction of HK from plasma (A) and of HK and HKa (B) from solutions of purified proteins by immobilized LM, VN and FN.(A) Blood ... ± 9.8 ( 5) 34.3 ± 0.9 ( 3) +Zn2+7.1 ± 3.0 ( 5) 4.6 ± 1.7 ( 4) HKNone 27.0 ± 4.6 ( 6) 22.2 ± 6.1 ( 4) +Zn2+8.7 ± 1.6 ( 4) 16.6 ± 5.7 ( 3) Table 2. The effect of Ca2+,Mg2+ and Zn2+on the binding...
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Báo cáo khoa học: dehydrogenase from Arabidopsis thaliana, a flavoprotein involved in vitamin C biosynthesis pot
... Oligonucleotideswere synthesized by Eurogentec (Liege, Belgium). The pET23a( +) expression vector and E. coli strain BL21(DE 3) were from Novagen (San Diego, CA, USA). All otherchemicals were from commercial ... was measured in the presence of the mediator 2,6-dichlorophenolindophenolat 600 nm, the activity with potassium ferricyanide wasmeasured at 420 nm (e420=1mm )1 Æcm )1 ), and the activitywith ... suggestingthat the holo form of the enzyme is more thermostablethan the apo form. Both local and global unfoldingplay a role in the thermoinactivation process. This isconcluded from the fact that,...
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Báo cáo khoa học: "Learning From Collective Human Behavior to Introduce Diversity in Lexical Choice" potx
... dj ). NT(dj ) DT(di ) ( 3) where NT(dj ) is the number of times the walk hasvisited djup to time T and DT(di ) =dj∈Vp0(di, dj)NT(dj ) ( 4) Here, p∗(dj ) is the ... (Erkan and Radev,200 4)) . Once the network is built, the system finds the most central sentences by performing a randomwalk on the graph.p(dj ) = (1 − ) 1|D|+ λdip(di)P (di→ dj ) ( 2) 5.2.3 ... both or neither, then the two annotators agreeon it, and otherwise they do not. Based on thisagreement setup, we can formalize the κ statisticas κ =Pr(a)−Pr(e)1−Pr(e)where P r(a) is the relative...
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Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc
... Mcl-1 and A 1), pro-apoptoticproteins (Bax, Bak and Bok) and BH3-only proteins(Bad, Bid, Bik, Blk, Hrk, BNIP3 and BimL). Bcl-2family proteins mostly mediate the activity of otherproteins in the ... phosphorylation of the MAPK family and mitochondrial proteins. Phosphorylated ERK prevents the activation of caspases and the Bcl-2 family, whereas these are activated by phosphorylated JNK and p38 MAPK, ... mitochondria and then translocate to the nucleus where they induce DNA fragmentation in a caspase-independent manner. Whether apoptosis is inducedor not depends on the activation balance of these proteins....
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Tài liệu Báo cáo khoa học: Time-dependent regulation analysis dissects shifts between metabolic and gene-expression regulation during nitrogen starvation in baker’s yeast doc
... manufacturer. The reactions contained5 lL of SYBR Green PCR Core Kit (Bioke, Leiden, The Netherlands), 3 pmol of each primer (Isogen, De Meern, The Netherlands or Biolegio, Nijmegen, The Netherlands) and ... understand the patterns ofenzyme capacities over time, we should rather considerthem as the result of a balance between protein synthe-sis and breakdown from the beginning. The differences between ... PFK and ALD were calculated by dividing the sum of the glycerol and ethanol fluxes by 2. The fluxesthrough the enzymes from GAPDH to ADH were taken tobe equal to the measured ethanol flux. As the...
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Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf
... one(or more) of them in the segment between positions402 and 408. In the complementary experiment, the C-terminal fragment of Rio1p (335–484p) was heavilyphosphorylated (Fig. 3C).These results ... a(encoded by CKA 1) and a¢ (encoded by CKA 2), and two regulatory b subunits, b and b¢ in Saccharomycescerevisiae (CKB1 and CKB 2). The enzyme occurs in allpossible combinations of a and b subunits ... [S402 (S 1), S403 (S 2), S409 (S 3), S416 (S 4), S417 (S 5), S419 (S 6)] consecutively numbered 1–6; cf. Fig. 1C (Fig. 6). The total number of CK2 phosphorylation sites of Rio1pwas deduced from experiments...
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Tài liệu Báo cáo khoa học: "Some Pragmatic Issues in the Planning of Definite and Indefinite Noun Phrases" potx
... activation actions: {l) whether the speaker intends that the hearer identify the denotation of the de- scription, and { 2) how much mutual knowledge the speaker and hearer share about the description's ... axiomatize the relationship that holds between the terms and the individuals they denote. The terms can consist of predicates combined with an iota operator, as in Lz D(z), where D{z) = D,(z) A ... strictly on the mutual belief of the speaker and the hearer at the time of the utterance. The noun phrase in a sentence such aa ( 6) Use the same wrench you used to unfasten the pump. must...
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Báo cáo khoa học: Cholesterol oxidase: biotechnological applications Loredano Pollegioni, Luciano Piubelli and Gianluca Molla pot
... Comparison of the stability of ChOxs, from differentsources, in the absence (A) and in the presence of 0.1% (B) and 0.5% (C) Triton X-100 (black bars) or sodium cholate (white bars). The enzyme ... (a) total and esterified serum;(b) from low-density lipoproteins to high-density lipo-proteins; (c) on the cell membrane of erythrocytes (and of other cells and cellular compartments); and (d) ... by adjust-ing the pH (from 6.5 to 7. 5), the temperature (from 39to 37 °C) and the agitation speed (from 200 to300 rpm) after 24 h of growth, maximal production ofChOx (340 UÆL )1 , corresponding...
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Từ khóa: bridging the gap between theory and practice of approximate bayesian inferencebridging the gap between theory and practice in integrated assessmentbridging the gap between theory and practice in journalismbridging the gap between theory and practice connecting courses with field experiencesbridging the gap between theory and practice language policy in multilingual organisationsbridging the gap between theory and practice in nursingBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018chuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMMÔN TRUYỀN THÔNG MARKETING TÍCH HỢP