Báo cáo khoa học: Transcriptional activity and Sp 1⁄3 transcription factor binding to the P1 promoter sequences of the human AbH-J-J locus docx

... organization of the human AbH-J-J locus and identiﬁcation of the transcription initiation sites proximal to the P1 promoter The structural organization of the human AbH-J-J locus is shown in Fig. 1. The ... attention on the activity of binding of nuclear extract to these boxes. Binding of nuclear factors to GC-rich boxes of the AbH-J-J P1 promoter In order to study protein–DNA interactions and fur-ther ... belonging to the Sp1 superfamily interact with the P1 promoter in intact HeLa cells.Effects of mutations of Sp1 -binding siteson P1- directed transcription In order to verify the role of Sp1 -binding...

Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf

... sero-type E and L2were of similar activity and both less activethan smooth LPS by a factor of 100 whereas, LPS fromChl. psittaci was of lower activity than the two otherchlamydial LPS. Nevertheless, ... ratio of 5 : 5 : 0.01 (v/v/v) and sprayed at a ﬂow rate of 2 lLÆmin)1.MALDI-TOF MS of LPS and free lipid A wereperformed on a Reﬂex III (Bruker-Daltonik, Bremen, Germany) in the linear TOF ... mMNaOD. Under these conditions,31P signals were sharp and proton signals were moredispersed. However, the signal of the anomeric carbon of the b-GlcpN was absent from the spectrum under theseconditions...

Báo cáo khoa học: Jackbean, soybean and Bacillus pasteurii ureases Biological effects unrelated to ureolytic activity docx

... added to the cotton meal,respectively (not shown). The time dependency of the entomotoxic effect was similar for both proteins, with a lag-phase of 3–4 days for death of the ﬁrst insects, and reachingmaximal ... characterize and to study the mode of action of entomotoxic ureases in order to establish theirbiotechnological potential against phytophagous insects.Contrasting with canatoxin, which is highly toxic ... differentmechanism, unrelated to the release of ammonia.We also compared the kinetic properties of these enzymeson the hydrolysis of urea and susceptibility to differenturease inhibitors. Even the highly homologous...

Báo cáo khoa học: Two conserved domains in regulatory B subunits mediate binding to the A subunit of protein phosphatase 2A pdf

... subunit, and one of avariety of r egulatory B subunits. These diverse B subunits in the P P2A heterotrimer allow the phosphatase to localize t odistinct regions of the cell and to dephosphorylate speci®csubstrates, ... level of nonspeci®c adsorption of the B56a polypeptides to the beads, and suboptimal binding in the absence of cotranslation of the A and C subunits. The smallest N-terminal fragmen t of B56a ... ops alter the binding of the B and C subunits [13]. The B subunitsbind to repeats 1±10 of the A subunit, whereas the C s ubunitbinds to repeats 11±15. Interactions between the B and Csubunits...

Báo cáo khoa học: Snail associates with EGR-1 and SP-1 to upregulate transcriptional activation of p15INK4b doc

... Snail and SP- 1, but not the association of Snailwith SP- 1.Chromatin immunoprecipitaion assay for in vivoDNA -binding activity of the candidate transcriptional factors To further examine whether ... transcriptional factors induced by TPA.Taken together, by examining the pattern of SII wesuggest that the in vitro DNA -binding activity of allthree transcriptional factors toward p15 proE/S couldbe ... the TPA-induced promoter activity of p15 –pro228 was slightly higher than that of p15 –profull and p15 –pro461, although its basal promoter activity decreased signiﬁcantly. Also, the promoter activity...

Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt

... with the higher inhibitory activity of the cEL peptide and the verylow inhibitory activity of the cYT peptide compared to other peptides. The ﬂanking residue of the b-turn, i.e. Tyr3 of the cVYpeptide ... suggestive of a b-turn in the peptide and the proximity of amide protons due to compact nature of the structure. However, t he coupling constant of most of the amide protons was in the range of 6–8 ... 2877measured by ﬂow-cytometry assay. Binding of Jurkat cells to SRBCs due to CD2 and CD58 interaction results in the formation of E-rosettes. The ability of each of the designed CD2 peptides to inhibit...

Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

... between the two isoforms of the protein,only the molecular mass of 50 kDa would suggest that wehad purified the truncated isoform.Cloning of the cDNA of two isoforms of tomatob-fructofuranosidase ... b-fructofuranosidase of tomato wasfurther confirmed by Foetisch et al. [22]. The aim of the present study was to analyze the role of N-linked glycans in the IgE-response of tomato-allergic patients using the b-fructofuranosidase ... structure to be the main glycan moiety of the protein. The carbohydrates of nLyc e 2 are able to triggerhistamine release from human basophilsIn order to confirm the clinical relevance of the tomatoallergen...

Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt

... any reactivity with the Toll-like receptors TLR-2 and -4, in contrast to control lipopeptides and LPS. Thesedata conﬁrm the applicability of our conformational concept of endotoxicity to nonlipid ... assay The LAL test is based on the property of lysates of amebocytes of the horseshoe crab Limulus polyphemus ,to form a solid gel in the presence of minute amounts of endotoxins. The comparison of ... Infrared spectrum in the spectral range 1800–900 cm)1(A) and in the range of the negatively charged phosphate band mas(PO2–) 1300–1190 cm)1(B)ofhydratedPC,MfGl-II,andLPSRe.3274 K. Brandenburg...

Báo cáo khoa học: Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design pdf

... affect the precise positioning of the aromatic ring of Tyr456 in the closed state. In the absence of a closed complex of E542K P2Ox, the effect of the loss of this hydrogen bond on the packing of the ... from the internalcavity of the homotetramer. Concomitantly, Phe454rotates and moves some 7 A˚ to ﬁll the active site and pack against the FAD cofactor. In the loop betweenb-strand B6 in the ... differences in the backbone position of the exposed head domain of up to 4.3 A˚, and of exposed parts of the Rossmann domain of up to 2.7 A˚, are observed. For the rest of the homotetramer-ic...

Báo cáo khoa học: Proper targeting and activity of a nonfunctioning thyroid-stimulating hormone receptor (TSHr) combining an inactivating and activating TSHr mutation in one receptor pptx

... alter the routing of the receptor to the cell surface, resulting in loss of basal activity and loss of agonist induced cAMP production [14–16].In order to study the mechanism of activation of the TSHr ... corresponds to the major unliganded form of the receptor; the active conformation capable of promotingGDP/GTP exchange on the G-protein is achieved by afraction of the unliganded receptors ... and (f) the mechanism by which binding of the hormone to the N-terminal domain of the receptor translates into activation of its serpentine domain is unknown.With the aim of improving our understanding...

Xem thêm