Báo cáo khoa học: Structural insight into the evolutionary and pharmacologic homology of glutamate carboxypeptidases II and III ppt

... bothGCPIII and GCPII, including the ‘glutarate sensor’ and the Pro-rich region [27]. Both GCPII and GCPIIIdisplay an induced-ﬁt movement of the ‘glutaratesensor’ upon binding of a glutamate (or glutamate- like) ... therapeutics and ligands, as well as in the investigation of the diverse biological functions of GCPIII and GCPII.Experimental proceduresrhGCPIII and rhGCPII expression and puriﬁcation The extracellular ... functional and pharmacological over-lap between GCPIII and GCPII, studies addressing the structural and physiological properties of GCPIII are crucial for obtaining a deeperunderstanding of the GCPII...

Tài liệu Báo cáo khoa học: Structural insights into the substrate speciﬁcity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

... erva-tamin-A, the P3 moiety of both the molecules of the asymmetric unit runs along the extended backbone of residues 65–64 and is partly exposed to the solvent(Fig. 2B). On the other hand, the P3 ... at the bottom of the subsite is conservedin ervatamins. Compared to a Ser in the equivalentposition of papain, the role of Leu here is to restrict the size and depth of the S2 subsite. The ... proton from the catalytic Cys pres-ent at the N-terminus of the helix to the His of the dyad [19,50,51]. The ﬁrst stage of catalysis is mediatedby the highly active thiolate ion of the Cys. Biochemi-cal...

Báo cáo khóa học: Mechanistic insight into the peroxidase catalyzed nitration of tyrosine derivatives by nitrite and hydrogen peroxide doc

... portions to the substrate solution during 1 h at 25 °C. Formation of the product was accompanied by the development of a yellow and persistent color in the solution. Then, the pH of the mixture ... excess of nitrite (from 40 lMto 1.6 mM). The compound II reduction to iron (III) followed a ﬁrst-orderbehavior. In order to decrease the noise in the readings, the determination of the observed ... presence of the phenol.Interestingly, these constants resemble the reciprocal of the nitrite binding constants to the proteins.According to Schemes 1 and 2, the nitrating speciesproduced by the...

Báo cáo khoa học: Structural basis for the changed substrate speciﬁcity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D docx

... ion in the P-loop and Glu172 to the 3¢-OH of the substrate. In the dTTPcomplex, the phosphates are bound by the LID argi-nines and the 3¢-OH is bound to Glu172. In thesecases, Glu171 in the LID ... comparing the phosphorylation of the natural substrates of the double mutant with the singlemutant, it seems that the mutation N45D in the double mutant counteracts the negative effect(s) of the N64D ... 3737actions with the 3¢-OH of the deoxyribose of the substrate. The absence of this hydrogen bond and aﬂexible LID make the substrate binding pocket larger and provide space for the bulky 3¢-azide...

Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

... 7. The arrowslabeled (b) and (c) point to the areas of interaction between the transmembrane helix of subunit 8 and helices G and H1 of cytochrome b and between the N-terminus of subunit 8 and ... panels A and B and the relative amounts of each of the subunits determined by densitometry scanning of the stained Westernblots of the q° and CKL57 membranes are shown in panels C and D, respectively.Ó ... inability of the truncated protein to insert into and be stable in the inner mitochondrial membrane or lack of detection of the truncated protein by the antibodies.In the W303–1B q° strain the amounts...

Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot

... alignment of the amino acidsequences of RVV-X HC with those of other represen-tative P -III enzymes. It shows highest similarity (82%)to VLFXA HC, and lower similarity to other P -III proteases, ... sequenced. Of these, 10clones were found to encode the LC2 and LC1 sub-units. Others were found to encode other variants of the C-lectin-like venom proteins. The amino acidsequences of both subunits ... signalpeptide of 23 residues, and the predicted sequence forits mature protein matches that published previously[8]. The amino acid sequences of LC1 and LC2, togetherwith those of other homologues of...

Báo cáo khoa học: New insights into the P-glycoprotein-mediated efﬂuxes of rhodamines doc

... the measurement of the rate of passive diffusion of the dye through the plasma membrane. This cannot be done by the simplemeasurement of the increase of the fluorescence signal(Fcyto) of the ... show that the efficiencies of the P-gp-mediatedefflux of Rh I, Rh II, TMR, Rh 6G, Rh B, RhI ,II hydro-lysed are similar to each other and to the efficiency of the P-gp-mediated efflux of anthracyclines. ... Na+-buffer on the one hand and K+bufferon the other hand. In Na+-buffer a strong decrease of the fluorescence signal was observed due to the accumula-tion of the lipophilic cation mainly in the mitochondrialcompartments,...

Báo cáo khoa học: Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron-encoded protein contribute to its maturase and intron ppt

... initiation of cDNAsynthesis. Because most of these mutants were able tocleave single- and double-stranded DNA substrates(Fig. 4), the impairment of mobility may reﬂect the requirement of these ... [2,20–25]. The L. lactis Ll.ltrBintron and the yeast aI1 and aI2 introns, which are the best studied mobile introns and serve as a paradigmfor group II intron mobility, all belong to the IIAclass. The ... most group II IEPs [13], and a putative short b-strand in the C-tail. The twoconserved domain X motifs are found at or near the C-termini of a2 and a3, respectively. The a-helices a1,a2 and a3...

Báo cáo khoa học: Recent insights into cerebral cavernous malformations: animal models of CCM and the human phenotype pptx

... helptranslate the cellular and biochemical insights into a better understanding of disease mechanism. In this minireview, we discuss the contributions of animal models to our growing understanding of the ... biology of cavernousmalformations, including the elucidation of the cellular context of CCMprotein actions and the in vivo conﬁrmation of abnormal endothelial cell–cell interactions. Challenges and ... san and vtn were later shown to exhibit the same pheno-type as the heg mutant ﬁsh – that of the dilated heartcovered by a single layer of myocardium. The similar-ity of the phenotype in these...

Báo cáo khoa học: Structural basis for the erythro-stereospeciﬁcity of the L-arginine oxygenase VioC in viomycin biosynthesis docx

... VioC•L-arginine•tar-trate•Fe (II) . The b-strands B, G, D, I and C build the major side of the jelly roll fold, and the minor side is built by the b-strands F, E and H. The flexible lid region is shown in blue, the bound ... contains threehelices (a1–a3) and one b-strand (A) parallel to the first b-strand, B, of the jelly roll core. The linkage of the fourth (E) and fifth (F) b-strand of the jelly rollfold is built ... Fe(IV)-peroxo or a Fe (III) -superoxospecies, which in turn attacks the 2-ketogroup of aKG. The following oxidative decomposition of a-KG formssuccinate and CO2 and leads to the formation of anFe(IV)-oxo...

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