Báo cáo khoa học: The regulation of the endosomal compartment by p53 the tumor suppressor gene docx

Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx

... in the previous sections for the special case of weak binding of the enzymes,holds more generally.Individual regulation of the phosphorylation sitesSo far, we have focused our analysis on the ... ﬁnely tuned by a single kinase. Takentogether, this study suggests a versatile regulation of protein activation by the combined effect of structural, kinetic and thermodynamic aspects of multisite ... another site, so that the phosphorylation kinetics of one residue can depend on the phosphorylation state of other residues in the pro-tein [8]. It is not clear how these factors modulate the response...

Báo cáo khoa học: Light regulation of CaS, a novel phosphoprotein in the thylakoid membrane of Arabidopsis thaliana doc

... 1Aand 4B) and the stn7 mutant (Fig. 4B). The involvement of STN8 in the phosphorylation of CaS was further investigated by isolation of phospho-peptides from the wild-type and the stn7 and stn8thylakoids, ... obtain further insights into the mechanismsresponsible for the observed phenotype, we analyzed the photochemical efﬁciency of PSII by ﬂuorescencemeasurements and the susceptibility of the CaS ... S2).Requirement of STN8 kinase for CaSphosphorylationTo address the question of whether one of the two light-regulated protein kinases, STN7 or STN8, isrequired for the light-dependent phosphorylation of APMCaSCaSFOX1D1Chl...

Tài liệu Báo cáo khoa học: Redox regulation of dimerization of the receptor proteintyrosine phosphatases RPTPa, LAR, RPTPl and CD45 pdf

... conditions. The ectodo-mains of both monomers in the dimer are required forthis effect, suggesting that the epitope tag of onemonomer is masked by the ectodomain of the othermonomer in the dimer, ... andsuggest an important role for the catalytic cysteine of D2 in the process.DiscussionWhether regulation of RPTPs by dimerization is ageneral feature is a subject of debate. There is ampleevidence ... Dixon JE (1993) Active site labeling of the Yersinia protein tyrosine phosphatase: the determi-nation of the pKa of the active site cysteine and the function of the conserved histidine 402. Biochemistry32,...

Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx

... The intracellular concentration of hemeis maintained by the rate of its synthesis and degrada-tion [1]. Many enzymes and their regulators areresponsible for heme synthesis [1,2]. On the otherhand, ... similar to that evoked by cadmium [36] orsodium nitroprusside [38], each of which activated the expression of a reporter gene under the regulation of the 4.5-kb HO-1 gene promoter. It should ... 6). On the otherhand, knockdown of either HO-1 or HO-2 expressionresulted in the accumulation of heme in the hemin-treated HepG2 cells. Taken together with the siHO-2-mediated induction of HO-1...

Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc

... control of the expression of the des cluster is mediated by the DmdR1 regulator.ResultsDeletion of the desA gene of S. coelicolor blocksdesferrioxamine biosynthesis The organization of the putative ... interesting that the )10 sequence islocated inside the iron box of desA (nucleotides 7–12 of the iron box), explaining the regulation of desAexpression by binding of DmdR1 to the iron box. The same ... transcriptional control of the desferrioxam-ine cluster.In this study, we report the deletion of the ﬁrst gene of the desABCD gene cluster (desA)inS. coelicolorA3(2), which caused cessation of desferrioxamine...

Báo cáo khoa học: Mechanical regulation of the Cyr61/CCN1 and CTGF/CCN2 proteins Implications in mechanical stress-associated pathologies pptx

... or generated by the cells results in profound alterations of the histo-morphometry, phenotype and function of the cells[3–7]. The onset of this process is characterized by the activation of ... induce up -regulation in the expression of the Cyr61 gene [71]. In line with theseresults it was shown in NIH 3T3 fibroblasts that the Cyr61 gene belongs to a group of target genes of serumresponse ... not clear whetherthere are any specific regulators at the receptor level of signal transmission, or whether targeting of Cyr61 andCTGF is achieved by specific cofactors at the level of cellular...

Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx

... mecha-nism of transphosphorylation, which implies the ABFig. 3. Stereo diagram of the active center of HtA. (A) Superposition of the active-siteresidues of the 20 conformers of the solu-tion ... superposition of the active site of RNase T1, a-sarcin and HtA is shown inFig. 3B. It is known that other side chains in the vicin-ity of these amino acids are also important for the activity. Given the ... surface area of the correspondingside chains very low. Desolvation of the chargedgroups should affect their pKavalues, increasing the pKa of carboxylates and decreasing the pKa of histi-dines...

Tài liệu Báo cáo khoa học: Abstract Integration of Metabolism and Survival PP-1 The metabolic switch in liver methionine metabolism pptx

... activity of APE1 and the thermal stability of DNAduplexes. The efﬁciency of the removal and the thermal stability of DNA duplexes varied depending on the reaction conditionsand the nature of the ... adduct of a molecular mass of around 100-kDa. Unexpectedly, the forma-tion of the 100-kDa adduct does not require the incorporation of the photoreactive dNMP residue at the 3’-margin of the nick ... dG in the MTase recognition sites. The BP lesions affect primarily the methylation reaction by decreasing/blocking the efﬁciency of the catalytic step rather than affecting the binding of the MTases...

Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... subunit and the d subunit is an intrinsic mole-cule of CaM that binds to the enzyme even in the absence of Ca2+[2]. The regulation of PhK throughCa2+⁄ CaM enables the coordination of muscle ... structural alignment of thesethree models shows that they are indeed close structuralTable 2. The quality of ﬁts (described by the chi-squared per degrees of freedom) for a series of Ca2+⁄ CaM ... as the time taken for the molecule to rotate through 1 radian or to translate through its own length. Dpara⁄ Dperpis the ratio between the length of the longs axis, z, and the length of the...

Tài liệu Báo cáo khoa học: NMR structure of AII in solution compared with the X-ray structure of AII bound to the mAb Fab131 pptx

... alignment of the fragment 4–7 of the 13 ensemble structures of AII to the fragment 3–6 of the X-ray structure of AII.Fig. 6. Structure of a representative folded conformer of AII showing the van ... to the average co-ordinates (the blue colour denotes the side chains of Arg2, Tyr4, Ile5, His6 and the yellow the side chains of Val3and Pro7) (a) with the the X-ray structure of AII in the ... between the twolimiting cases. The intermediate values (6.4 Hz) of the backbone couplings for Tyr4 and His6 are possibly the result of twisting or bending in the middle of the amino-acidsequence of...

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