... 4601
MINIREVIEW
Amyloid structure – one but not the same:
the many levels of fibrillar polymorphism
Jesper S. Pedersen
1
, Christian B. Andersen
2,3
and Daniel E. Otzen
4
1 Department of Biochemistry, Molecular ... release
from the cells, these amyloid structures would gradu-
ally dissociate into active monomers in the blood-
stream [17], which would imp...
... annotation, in terms
of the edit distance from the manual annotation,
among the 10-best hypotheses of the FST model;
(C) as above but s
i
is selected among the 100-
best hypotheses. The pairs are also ... we noticed that many times the
hypothesis ranked first by the SCLM is not the
closest to the correct concept sequence, i.e. its er-
ror rate using the Levensht...
... variations in the level of endogenous
Ruk L, suggesting a long half-life for the protein (Fig. 4C).
Furthermore, we examined the effect of selected inhibitors
of protein degradation on the level of endogenous ... retains only
the C-terminal coiled-coil region.
Although the precise role of this Ruk/CD2AP family is
still unknown, they are involved in the control of apo...
... the tk promoter, although the extent of
activation differed among these segments. For the seg-
ments from T4 to T32, the extent of transcriptional acti-
vation correlated with the length of the ... expression of transgenes,
which is of importance in many areas of the biological
sciences. Moreover, such technology could lead to the
development of useful nonviral...
... and B) in the asym-
metric unit display the canonical cystatin fold, (N)–b 1–
a–b2–L1–b3–AS–b4–L2–b 5–( C), with a five-stranded
antiparallel b-sheet gripped around a long a-helix
(Fig. 1). The AS is ... the presence of this extra bridge
does not perturb the conformation of the main chain in
the region of these point mutations. This is possible
because the side cha...
... potential
(E°) of the substrate must be low enough, as the rate
of the reaction has been shown to depend on the dif-
ference between the redox potentials of the enzyme
and the substrate (DE °) [2 8–3 1].
This ... attached to
the T2 copper, as observed in the crystal structure of MaL. The unique fea-
ture of TaLcc1 and MaL as compared with other laccases struc...
... and amide
backbone groups. The one exception to this is the
location of the amide group of Lys204 at P3, where
there is a break in the main-chain density at the 2.8r
map level. The corresponding ... primarily occurs by the formation of a hydrogen
bond between the side-chain hydroxyl group of Tyr205
and the importin-a main chain at the C-terminus of
the ARM1 H...
... [26]. These findings
lead to the hypothesis that Fe-bound ApeSOD mimics
the product-inhibited form and the shift of Tyr39 sup-
presses the release of the peroxide product. This may
be one of the ... the ter-
tiary structure of ApeSOD has not been elucidated.
In the present study, for the first time, we describe the
crystal structure of ApeSOD. In particular...
... well. The hydrogen bond between
the backbone nitrogen atom of Asp291 and the 3-
phosphate of the substrate is the only interaction with
the protein backbone; all other contacts are formed
using the ... identified. These are not part of the predicted
binding mode of phytate to PhyK. Out of the water
molecules included in the structure none is bound in
the cat...
... of intrinsically dynamic behav-
ior. These regions correspond to D11–E14, A46–R51,
G53–C57, K83–G89, S101–A104, D117–N119 and
G122–F125.
Description of hirsutellin A structure
The structure of ... transphosphorylation, which implies the
A
B
Fig. 3. Stereo diagram of the active center
of HtA. (A) Superposition of the active-site
residues of the 20 conformers of t...