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Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx

Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx

Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx

... 237 amino acids 15 amino acids ⁄ 14 amino acids 25 kDa ⁄ 4.07PaTry2 GU338028 870 798 nucleotides 58 nucleotides AATAAA ⁄ 10 nucleotides 266 amino acids ⁄ 237 amino acids 15 amino acids ⁄ 14 amino ... nucleotides ATTAAA ⁄ 13 nucleotides 278 amino acids ⁄ 249 amino acids 15 amino acids ⁄ 14 amino acids 27 kDa ⁄ 4.6 New members of the brachyurins family in lobster E. Perera et al.3498 FEBS Journal 277 ... AATAAA ⁄ 13 nucleotides 266 amino acids ⁄ 237 amino acids 15 amino acids ⁄ 14 amino acids 25 kDa ⁄ 4.07PaTry1b GU338027 873 798 nucleotides 61 nucleotides AATAAA ⁄ 13 nucleotides 266 amino acids...
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Báo cáo khoa học: Possible involvement of an FKBP family member protein from a psychrotrophic bacterium Shewanella sp. SIB1 in cold-adaptation potx

Báo cáo khoa học: Possible involvement of an FKBP family member protein from a psychrotrophic bacterium Shewanella sp. SIB1 in cold-adaptation potx

... monitoring the change in the concentration of p-nitroaniline. The increase in the rate of isomerization is implicit in the increased rate of p-nitro-aniline release, because catalysis of isomerization ... cellularcontents of the proteins that are associated with adaptationat these conditions usually increase. More specifically, the cellular content of a protein that is involved in cold-adaptation, may increase ... ligating the DNA fragment containing the Sh-fklB gene into the NdeI–BamHI sites of pET-2 8a. This DNA fragment wasamplified by PCR. The sequences of the PCR primers were5¢-AGAGAGAATTCATATGTCAGATTTGTTCAG-3¢for...
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Báo cáo khoa học: New application of firefly luciferase ) it can catalyze the enantioselective thioester formation of 2-arylpropanoic acid docx

Báo cáo khoa học: New application of firefly luciferase ) it can catalyze the enantioselective thioester formation of 2-arylpropanoic acid docx

... differentiate the chirality of 2-phenylbutanoic acid and the ee value of unreacted acid was almost the same as the case of 2-phenylpropanoic acid. In the case of 2-hydroxymethylpropanoic acid (tropic acid) ,which ... ketoprofenyl-CoA catalyzed by LUC-H was examined to obtain the detailed reaction information. The efficiency of thioest-er formation was calculated from the peak areas of the remaining acid and ... long-chain fatty acids. This report indica-ted that firefly luciferase also has an acyl-CoA synthe-tase activity toward an unnatural substrate. In addition, Nakamura et al. [10] showed that luciferaseexhibits...
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Tài liệu Báo cáo khóa học: New activities of a catalytic antibody with a peroxidase activity ppt

Tài liệu Báo cáo khóa học: New activities of a catalytic antibody with a peroxidase activity ppt

... change of the Fe(II) spin state and noreplacement of any of the two axial ligands of the iron,His18 or RNO, by an amino acid side-chain of the antibody protein.Binding site topology of antibody ... the binding of a ligand, such as nitrosoalcane, to the iron of MP8, it brings a partial steric hindrance on the distal face of MP8 and thus controls access of the nitrosoalcane ligandto the iron atom of ... S-oxidation.Catalytic antibodies with a metalloporphyrin cofactor, orÔhemoabzymesÕ, are not as efficient a category of catalysts astheir natural hemoprotein counterparts. The hemoabzymes,which display a...
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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc

Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc

... indolepyruvatedecarboxylase fromEnterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid Anja Schu¨tz1, Tatyana Sandalova2, Stefano Ricagno2, Gerhard ... decarboxylase,suggesting that the interactions with the cofactor thiamindiphosphate and the catalytic mechanisms are very similar. The substrate binding site in indolepyruvate decarboxylasecontains ... residues marked ÔdÕ), with most residuesat the interface coming from the PYR and PP domains. Thisis in agreement with the average mobility of the domains in the crystal; the PYR domain has the lowest...
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Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: Blue-light active flavoproteins studied by electron paramagnetic resonance pptx

Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: Blue-light active flavoproteins studied by electron paramagnetic resonance pptx

... yields information on the chemical nature of the individual radicals of the radi-cal pair state, and their interaction with each otherand with their immediate surroundings.EPR ⁄ ENDOR investigations ... spectrum of the flavin radical of a protein-bound FMN radical of the LOV1 domain (C57M mutant) of Chlamydomonas reinhardtii phototropin [34]. Experimental and calcu-lated EPR spectra are shown as solid ... otherproteins involved in intracellular signaling or localiza-tion [24,25]. In photolyases and cryptochromes, radicals andradical pairs play a prominent role in their function. In nearly all cases, these...
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Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: Histone demethylase LSD1 and chromatin pot

Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: Histone demethylase LSD1 and chromatin pot

... (peroxisome)LAAO (snake) 19 4.0L -amino acids amino acids metabolism (venom toxin?)GOX (bacterial) 14 4.0 glycine, sarcosine amino acids metabolismMSOX (bacterial) 10 4.3 sarcosine amino acids metabolismFlavin-dependent ... with other flavin amine oxidases, including the overall fold of the amine oxidasedomain region and details in the active site that are relevant for aminesubstrate oxidation.AbbreviationsAOD, amine ... cofactor(either the re or the si face depending on the enzyme) ,which has alongside one or two aromatic amino acidsthat form a sort of cage together with the flavin ring. In PAO and in MAO A, ...
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Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: An unexpected role for quinone reductases as regulators of proteasomal degradation pptx

Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: An unexpected role for quinone reductases as regulators of proteasomal degradation pptx

... state governs the association of Yap4p and Lot6p. Although it appears likely that the redox state of the FAD cofactor of NQO1 andNQO2 plays a similar role in the mammalian system,unequivocal ... acid side chains (Fig. 2) [68]. As mentioned in the introduc-tion, QRs also adopt a flavodoxin-fold and the isoal-loxazine ring engages in a similar interaction with a peptide chain. In the reported ... inhibitorthat pi-stacks on top of the isoalloxazine ring system,preventing association with p53 [44,67]. Similarly,removal of the flavin in Lot6p disables the interaction with the yeast transcription...
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Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt

Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt

... CAGACTAGTGGTTAGAGGAGAGGAATGATGCTGTAGAGACADENV-1 92.1 ± 0.57 73.6 ± 2.311G4P217 ATATGCTGAAACGCGTGAGCATCATGAGACAGAGCGATDENV-3 90.3 ± 1.09 83.9 ± 5.161G5P30 TTCCAACAAGCAGAACAACATGCTACAGGCAGCACGGTTTDENV-4 ... Group 4 ATATGCTGAAACGCGTGAGCATCATGAGACAGAGCGATDENV-3 1043822791G5P30 Group 5 TTCCAACAAGCAGAACAACATGCTACAGGCAGCACGGTTTDENV-4 990310318415C. Gijavanekar et al. PCR detection of nearly any ... Wedemonstrated the broad strain sensitivity of thisreagent using DENV cDNA clones and RNA of the four serotypes in the presence of a vast excess of human DNA and RNA. The reagent has high analyti-cal...
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Báo cáo khoa học: Molecular imprinting of cyclodextrin glycosyltransferases from Paenibacillus sp. A11 and Bacillus macerans with c-cyclodextrin pptx

Báo cáo khoa học: Molecular imprinting of cyclodextrin glycosyltransferases from Paenibacillus sp. A11 and Bacillus macerans with c-cyclodextrin pptx

... (Osaka, Japan). BM CGTase was obtainedfrom Amano Enzyme Inc. (Aichi, Japan) and had a specificactivity of 1003 UÆmg)1 of dextrinizing activity [13]. A1 1CGTase was purified using starch adsorption ... resulting from an increase in the turnover rate (kcat) and the binding affinity (Km)(Table 3). The Kmvalues of the CLIP CGTases in the coupling reaction indicated stronger binding of the CD8substrate, ... combined and lyophilized.Determination of free amino groups of the CGTases The relative amounts of amino groups of the native andcovalently derivatized CGTases were determined accordingto Habeeb...
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