Báo cáo khoa học: Curcumin suppresses the dynamic instability of microtubules, activates the mitotic checkpoint and induces apoptosis in MCF-7 cells ppt

... 3437–3448 ª 2010 The Authors Journal compilation ª 2010 FEBS Curcumin suppresses the dynamic instability of microtubules, activates the mitotic checkpoint and induces apoptosis in MCF-7 cells Mithu ... with vinblastine in inhibiting MCF-7 cell proliferation. Curcumin affected the localization of the kinesinprotein Eg5Because curcumin produced monopolar spindles in MCF-7 cells, we examined the ... lm.Tubulin DNAMergeControl Curcumin 24 μM Curcumin 36 μMControl Curcumin 0 min15 min15 min25 min25 min0 minABFig. 2. Curcumin- perturbed mitotic spindle structures of MCF-7 cells. (A) MCF-7...

Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

... proteins they probably formpart of the copper binding sites in this protein.Although there are four histidines in the C-terminal,another histidine is present at position 18, and given the folded ... withglycine alone was performed and no binding was observed. In our hands, as others [54], the most reproducible datawas obtained from injections of the metal into a solution of the protein. Typically ... high affinitycopper binding protein, and (b) that amyloid fibrilformation of this protein is diminished in presence of Cu2+ions. Another interesting finding is thatcopper binding gets less strong...

Báo cáo khoa học: Differential tissue-speciﬁc distribution of transcripts for the duplicated fatty acid-binding protein 10 (fabp10) genes in embryos, larvae and adult zebraﬁsh (Danio rerio) docx

... distribution of fabp10a and fabp10b transcripts in developing and adult zebrafish, evidence of the divergence of regula-tory elements in the promoters of the fabp10a and fabp10b genes compared with the ... shown in capitalletters, with the coding sequences of eachexon underlined and the deduced aminoacid sequence indicated below. The nucleo-tide positions in the gene sequence areindicated by the ... were detected only in the liver,intestine and testis of adult zebrafish [6].Are the duplicated fabp10 genes retained in the zebrafish genome owing to the neofunctionalization of fabp10b?Based...

Báo cáo khoa học: "Adding Syntax to Dynamic Programming for Aligning Comparable Texts for the Generation of Paraphrases" potx

... it marks the beginning of a noun-phrase (NP). On the other hand, “Theater” has anIOB value of I-NP because it is inside a noun-phrase (Milan Theater) and is not at the beginning of that constituent. ... with the overall most similar pair and then respect the initial ordering of the cluster,aligning remaining sentences sequentially. Eachsentence is aligned against its best match in the pool of ... constituent. Finally, the syntactic depen-dence trace of a word is the path of IOB valuesfrom the root of the tree to the word itself. The last element in the trace is hence the IOB of the word...

Tài liệu Báo cáo khoa học: Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocase docx

... couldparticipate in proton translocation or, in the case of E103, in the binding of the twin-arginine motif in the signal peptide. The absence of any translocationdefects leads us to conclude that these ... synthesis with cleavable, N-ter-minal signal peptides that almost invariably contain anessential twin-arginine motif in the N-terminal domain[9,10]. They then interact with a translocon in the inner ... 2275Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocaseClaire M. L. Barrett and Colin RobinsonDepartment of Biological...

Tài liệu Báo cáo khoa học: "Topic Models for Dynamic Translation Model Adaptation" pptx

... set-ting, or in one domain, is not necessarily preferable in another domain. Indeed, sometimes the domaincan change the meaning of a phrase entirely. In a food related context, the Chinese ... (2011) extends the same notion of condition-ing on provenance (i.e., the origin of the text) by re-moving the separate mapping step, directly optimiz-ing the weight of the genre and collection ... distribution of the sentence from which we areextracting the phrase. To optimize the weights of these features we combine them in our linear modelwith the other features when computing the modelscore...

Tài liệu Báo cáo khoa học: "An Information-Theory-Based Feature Type Analysis for the Modelling of Statistical Parsing" docx

... headword of the node. In the following, the units of PIQ are bits. ConclusionAmong the feature types in the same structuralposition of the parsing tree, the predictiveinformation quantity of lexical ... sequencecontaining the leaf nodes of the partial parsingtree rooted by the current node, representing the final objectives to be derived from the currentnode.4.2 The corpus used in the experiment The ... brother)0.2505(Y= the first left brother of the parent)0.0949(Y= the second left brother)SD=2 0.6483(Y= the grandpa)0.1066(Y= the second right brother)0.1068(Y= the first rightbrother of the parent)...

Tài liệu Báo cáo khoa học: A single amino acid substitution of Leu130Ile in snake DNases I contributes to the acquisition of thermal stability A clue to the molecular evolutionary mechanism from cold-blooded to warm-blooded vertebrates docx

... AB045037 and EMBL AB041732 were obtained. Phylogenetic treeswith the nt sequence of the open reading frame (ORF) of their cDNAs and the corresponding aa sequences, in Table 1. Summary of the puriﬁcation ... susceptible. These ﬁndings show that the presence of Val67 is one of the essential factors responsible for the actin-binding capacity of these snake DNases I.Tissue distribution of snake DNases I The ... including the kidney, liver and stomach, as wellas the pancreas and small intestine [2,7,19].cDNA structures encoding three snake DNases I and expression of the DNase I cDNAs in COS-7 cells The...

Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx

... reinforce the argument that the basic domain should be exposed at the surface of the Tat-containing recombinant proteins and call other reasonsto explain the differences in the mechanism of internaliza-tion ... appearsunlikely. The number of arginine residues within the Tatpeptide appeared to be the main determinant for main-taining a high translocating activity as pre viously shown byalanine-arginine substitution ... amounts of the rhodamine-labeled Tat peptide, as indicated in the ®gure.Fig. 2. FACS analysis of Tat peptide and Tat±GFP fusion protein uptake in HSexpressing or de®cient cell lines. Plain linesin...

Báo cáo khoa học: CD91 interacts with mannan-binding lectin (MBL) through the MBL-associated serine protease-binding site doc

... shown).Determination of the kinetic constantsTo determine the kinetic constants of the interactionbetween CD91 and MBL, increasing concentrations of recombinant MBL, ranging from 3 to 30 nm, wereinjected ... 2010 The Authors Journal compilation ª 2010 FEBSligand-binding clusters and an 85 kDa beta-chaininvolved in endocytosis and comprising the transmem-brane and intracellular domains. Ligand interaction ... between CD91 and MBL and CD91 and L-ficolin could be inhibited by ligands of both CD91 (RAP, C1q) and MBL (MASP-3) and therefore fulfilled the requirements of a specific inter-action. The binding between...

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