... transitions of the nucleo-tide-binding cleft. For this purpose, we studied the effects of the D-loop cleavage on the thermal unfold-ing of G- and F-actin. Previously, the thermal unfold-ing of G-actin ... conforma-tion of the interdomain cleft in the cleaved actin com-pared to intact actin, both in the monomers and in the filament subunits.ResultsEffects of the D-loop cleavage on the thermalunfolding of ... [6,27,43,44].Thus, the increased thermal stability of G-actinappears to correspond to the closed conformation of the nucleotide-binding cleft, whereas the decreasedthermal stability is a feature of the actin...