... reduction in hydrolysis by two orders of magnitude.AbbreviationsARCA (anti-reverse cap analog), m27,2¢-OGpppG and m27,3¢-OGpppG; bn7GpppG, 7-benzylGpppG; BODIPY, 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; ... compilation ª 2010 FEBSwith m7GpppBODIPY, GpppBODIPY and ApppBO-DIPY (BODIPY, 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene), but not with natural caps m7GpppG orm32,2,7GpppG. Methylated mono- ... obtained for m27,2¢-OGpppG and m27,3¢-OGpppG demonstrated the crucial role ofthe 2¢-OH and 3¢-OH groups of the m7Guo moiety forC. elegans DcpS hydrolysis. The 2¢-O-Me and 3¢-O-Me0 10 20 30...