Báo cáo khoa học: AdipoR2 is transcriptionally regulated by ER stress-inducible ATF3 in HepG2 human hepatocyte cells pdf

... analyzed in ER stress-induced HepG2 cells determined by westernblot.Table S2. Densitometric values of proteins analyzed in HepG2 cells by western blot.Table S3. Statistical analysis of arbitrary ... activity. This adipokine has amelio-rative effects on insulin resistance in peripheral tissues,and plays a central role in the regulation of energyKeywords AdipoR2; ATF3; ER stress; insulinresistance; ... Changes in the promoter activity of AdipoR2 under conditions of ER stress and ATF3 over-expression. (A) Activity of the AdipoR2 pro-moter in HepG2 human liver cells with ER stress induction by 1.0...

Báo cáo khoa học: Pfkfb3 is transcriptionally upregulated in diabetic mouse liver through proliferative signals docx

... streptozotocin (STZ) in rodents, which degeneratespancreatic insulin-producing b -cells [2]. This model is characterized by decreased plasma insulin levels, severehyperglycaemia and alterations in insulin-dependentsignal ... performedto distinguish between perivenous and periportalhepatocytes [30]. UPFK-2-overexpressing hepatocyteswere predominantly located in the perivenous zone [31]of the liver (Fig. 5C, merged).Mouse ... uPFK-2 is overexpressed in perivenous diabetic liver zones, asassessed by hydrodynamically delivered GFP as aperivenous marker. In the liver, hepatocytes are notterminally differentiated in normal...

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... Forphosphatidylserine determination, cells were washed withbinding buffer and incubated with Annexin V-FITC for15 min at room temperature. Cells were deposited immedi-ately in poly(l-lysine)19coated ... molecules.Herein, we report that parameters of iron metabolism were regulated in acoordinated-fashion in a human hepatoma cell line (HepG2 cells) after ironloading, leading to increased cellular ... Yet, HepG2 cells grown in iron-rich mediashowed a further increase in ROS production as deter-mined by an increase in DCFH mean ﬂuorescenceintensity (Fig. 2B, thick line). In addition, determin-ation...

Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx

... fate ofcreatine that is being synthesized by tumor cells couldnot be determined precisely. Furthermore, there wereno signiﬁcant differences in ornithine and creatininecontent in the blood ... important one in tumor cells is the conversion of ornithine to putrescine, which is the precursor molecule of polyamines such as spermi-dine and spermine. This reaction is catalyzed by ornithine decarboxylase ... expression were high in tumors. This indi-cates that the pathway of polyamine formation fromarginine metabolism is favored in this tissue. Again,arginase II, which is a mitochondrial enzyme, is highlyexpressed...

Báo cáo khoa học: Stimulation of ﬁbroblast proliferation by neokyotorphin requires Ca2+ inﬂux and activation of PKA, CaMK II and MAPK/ERK pdf

... neokyotorphin stimulatedproliferation, it had no effect in medium containing nofetal bovine serum. Thus, in L929 cells, neokyotorphindoes not induce mitosis, rather it acts by enhancingthe effect ... (5000–8000 cells per well). After 18 h of subculture, fetal bovine serum-supplied medium was replaced by fetal bovine serum-free medium containing test substances. In control samples, fetal bovine serum-free ... probable, as it is PKA dependent [30].MSK1 is a cAMP-response element binding protein(CREB)-phosphorylating kinase [31]; however, CREBphosphorylation by MSK1 in ﬁbroblasts is induced by stress factors...

Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot

... 2-vinylpiridine at roomtemperature for 60 min. In a typical experiment, 0.15 mL ofsupernatant was treated with 3 lL of undiluted 2-vinyl-pyridine. Nine microliters of triethanolamine were ... (1991) Reversal of age-related increase in brain protein oxidation, decrease in enzymeactivity, a nd loss in temporal an d s patial m emory by chronicadministration of the s pin trapping compound ... measurements of intracellular ﬂuores-cence. Dead cells were excluded by electronically gating dataon the basis of forward- vs. side-scatter proﬁles; a minimumof 1 · 104 cells of interest were analysed...

Báo cáo khoa học: Immunonanoparticles ) an effective tool to impair harmful proteolysis in invasive breast tumor cells docx

... asdetermined by staining with nigrosin. Caco-2 cells werecultured in minimal essential medium supplemented with2mm glutamine, 1% nonessential amino acids and 10%N. Obermajer et al. Cystatin-loaded ... with anti-cytokeratinmonoclonal IgG were added and the cells were incubatedfor different time periods.To determine speciﬁc uptake, MCF-10A neoT and Caco-2 cells were plated either in a mono- ... enter tumor cells, accumulating in the lysosomes, is necessary.Polymeric nanoparticles comprise promising systemsfor delivering antitumor agents known for their rapidinternalization into highly...

Báo cáo khoa học: A spectroscopic study of the interaction of isoﬂavones with human serum albumin pdf

... ring A of genistein. Therefore, the recognition ofunique binding sites in HSA by genistein and warfarin is due to signiﬁcant structural differences in ring B andsuch a characteristic binding ... strength on binding of genistein–HSA interactionTo determine whether ionic interactions play a role in the genistein–HSA interaction, the ionic strength ofthe buffer was increased by the addition ... genistein binding in presence of warfarin suggest that the binding is inclusive. There is enough conformational ﬂexibility in domain IIA of HSA to accommodate both warfarinand genistein. The binding...

Báo cáo khoa học: Functional role of Bb-chain N-terminal fragment in the ﬁbrin polymerization process pdf

... FEBSperipheral regions of the fibrinogen molecule were his-torically named D-domains. However, it was discov-ered by X-ray analysis that there were two distinctbC- and cC-domains in the peripheral ... BbArg14 is involved in another fibrin poly-merization site localized at the N-terminal fragment ofthe fibrin Bb-chain. This site is operational beforefibrinopeptide B is split off.The inhibitory ... by pro-line brackets, which usually indicates that such pep-tides are involved in protein–protein interactions [17].Three mAbs to t-NDSK that inhibit fibrin polymeriza-tion are described in...

Báo cáo khoa học: "The clinical value of daily routine chest radiographs in a mixed medical–surgical intensive care unit is low"

... The Netherlands7Anaesthsiologist-intensivist, Department of Intensive Care Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands8Internist-intensivist, Research ... Netherlands4Clinical Epidemiologist, Department of Clinical Epidemiology and Biostatistics, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands5Internist-intensivist, ... theforms during the study period.It was unit policy to obtain CXRs after insertion of endotra-cheal tubes, intravenous lines and chest drains, but not afterinsertion of nasogastric tubes. In addition,...

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