Báo cáo khoa học: Selection of full-length IgGs by tandem display on ﬁlamentous phage particles and Escherichia coli ﬂuorescence-activated cell sorting screening doc

... Authors Journal compilation ª 2010 FEBS 2303 Selection of full-length IgGs by tandem display on filamentous phage particles and Escherichia coli fluorescence-activated cell sorting screening Yariv Mazor1,2, ... permitting the selection of full-length IgGs from libraries displayed on phage. We believe that sequential selection by phage display and then FACS enables the efficient screening of verylarge ... advan-tage of display of full-length IgGs on filamentous phage particles as aprescreening step to reduce library size and enable subsequent rounds of FACS screening in Escherichia coli. For the...

Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"

... attentional failures after they had been working formore than 16 hours on the traditional schedule [2].LetterEffect of intern’s consecutive work hours on safety, medicaleducation and professionalismChristopher ... hours or more consecutive workare unsafe. Efforts to reduce work hours should focus first and foremost not on the frequency of extended-duration workshifts, but on the duration of consecutive work ... interventions on the performance of upper-levelresidents and other medical staff across a variety of disciplines. We likewise agree that optimizing patient hand-offs, medical education, and trainees’...

Báo cáo khoa học: Suppression of microtubule dynamics by benomyl decreases tension across kinetochore pairs and induces apoptosis in cancer cells potx

... in HeLa cells. (A) Cleavage of PARP by benomyl treatment. HeLa cells were treated with the indi-cated concentrations of drugs for 40 h and equal amounts of cell lysates were resolved by SDS ⁄ ... ratio of the width to the height of the chromosomal mass (congression index) of HeLa cellswith metaphase type chromosome alignment in the absence and presence of different concentrations of the ... density of 5 · 104cellsÆmL)1 and grown in 24-well tissue culture plates 24 h before theaddition of benomyl. Cells were then grown in the absence and presence of different concentrations of benomyl...

Tài liệu Báo cáo khoa học: Selection of stably folded proteins by phage-display with proteolysis docx

... steps: ﬁrst, generation of multiple coremutants of the target protein; secondly, display of mutants on the phage surface; and ﬁnally, selection forstably folded mutants by challenging the system ... that proteins can bestrongly stabilized by many different sets of surfacemutations. Furthermore, the selection is found to bedependent on selection conditions. In the ionic denaturant(guanidinium ... surface-exposed positions. In their study, six of thesepositions were randomized by saturation mutagenesis, inwhich any of the 20 amino acids can occur at each of thesix positions. Selection was made...

Báo cáo khoa học: Selection of a CXCR4 antagonist from a human heavy chain CDR3-derived phage library doc

... evalu-ated by measuring the effect of the phage on cAMPproduction. None of the ECL2-speciﬁc phage clonesdisplayed agonistic properties. The antagonistic prop-erties of the selected HCDR3 phage ... distribution of the subpopula-tion of phage isolated after each selection round wasmonitored and compared with the length distribution of the initial library. Analysis of the phage populationisolated ... set of conventional random peptide libraries for the identiﬁcation of CXCR4antagonists using a peptide corresponding to the second extracellular loop of the receptor CXCR4 as target. A total of...

Báo cáo khoa học: Selection of peptides inhibiting a b-lactamase-like activity docx

... two-step selection procedure from a random library of constrained peptidesdisplayed on bacteriophages, based on exclusive affinity fortheIgactivesite.Materials and methodsProduction and purification ... 7.5, containing 50 mMTris/HCl and 150 mMNaCl. The library was stored at )20 °C.Titration of the library was performed by infection of mid-log phage E. coli bacteria by dilutions of phages ... the first and the second round of negative selection in our experimental conditions. On the one hand,there is an increase in the yield of specific peptides recovered,but on the other hand the...

Tài liệu Báo cáo khoa học: Plasticity of laccase generated by homeologous recombination in yeast docx

... strong conservation of theoriginal LAC3 properties upon recombination,although the protein sequence of this hybrid is the leastrelated to that of LAC3 (Fig. 5). On the other hand,LAC131 and ... rate of 4 mLÆmin)1with a step gradient of NaCl: 0.1, 0.15, 0.2, 0.25, 0.3 and 1 m. Fractions containinglaccase activity were pooled and concentrated to a volume of 600 lL by ultraﬁltration on ... buffer con-taining no salt, concentration and addition of 15% glycerolwere undertaken for long-term storage of the protein()20 °C). Enzyme purity in active fractions was then con-ﬁrmed by SDS–PAGE.Standard...

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... and diploid cells [8,9]. During filamentation,Cdc42 regulates cell morphogenesis and invasion of the substratum [10].Among the Cdc42p effectors that regulate cell polari-zation are Ste20p and ... growth. (A) Morphology of normal and hyperelongated cells. Exponentiallygrowing cells carrying pGAL1-CLN1-3HA on a plasmid were induced by the addition of galactose for 4 h. The cells were then fixedwith ... Deletion of CLA4 results in higher amounts of SE. (A) Quan-tification of SE in cell polarity mutants. Cells of the indicated strainswere grown to stationary phase and then lipids were extracted and separated...

Tài liệu Báo cáo khoa học: "Evaluation of Machine Translations by Reading Comprehension Tests and Subjective Judgments" doc

... tests and advised on preparation of the final text, and to D. B. Robinson, Jr., L. Rosier, and B. J. Kinsberg for their contributions to selection of passages and preparation of questions ... values of a set of stimuli along a judged continuum is known to affect the distribution of responses for all stimuli in the set. The additional effect of context, on the other hand, would be ... three categories of response are shown separately. Method of translation and judgment condition are indicated by different patterns. assigned to the categories and the values of the judgments...

Báo cáo khoa học: Effects of a novel arginine methyltransferase inhibitor on T-helper cell cytokine production pot

... indicated by ‘+’) before methylation reac-tions using calf thymus histones as a source of thePRMT1 substrates histone 4 and histone 2A [18]. Inhi-bition by both compound 4 and AMI-1 was relieved by ... reduced the production of IFN- c from Th1cells by more than 60% and the levels of IL-4 fromTh2 cells by more than 75%, while incubation withAMI-1 reduced Th -cell cytokine expression by lessthan ... identiﬁcation of one such com-pound and the characterization its inhibitory proper-ties, focusing on its modulation of Th cell function.ResultsChemistryIn the report disclosing the activity of AMI-1,...

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