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Báo cáo khoa học: Reducing expression of NAD+ synthesizing enzyme NMNAT1 does not affect the rate of Wallerian degeneration ppt

Báo cáo khoa học: Reducing expression of NAD+ synthesizing enzyme NMNAT1 does not affect the rate of Wallerian degeneration ppt

Báo cáo khoa học: Reducing expression of NAD+ synthesizing enzyme NMNAT1 does not affect the rate of Wallerian degeneration ppt

... dowregulation of NMNAT1 expression does not affect the rate of axon degeneration in vitro.DiscussionThese data indicate that complete NMNAT1 geneinactivation is incompatible with the normal develop-ment ... Journal compilation ª 2011 FEBS 2679 Reducing expression of NAD+ synthesizing enzyme NMNAT1 does not affect the rate of Wallerian degeneration Laura Conforti1,2,3, Lucie Janeckova1, Diana Wagner2, ... develop-ment of embryos, as the extranuclear isoformsNMNAT2 and NMNAT3 cannot compensate forcomplete loss of NMNAT1. Nmnat1 + ⁄ )mice havereduced NMNAT1 expression and enzyme activity;however, they...
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Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform specificity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx

Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform specificity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx

... apparentadditivity of the stoichiometries of the complexes ren-dered by the separate moieties of CeMT1 andCeMT2, the summation of their CD spectra did not give rise in any case to spectra close to those of the entire ... for the native and recombi-nant Cd(II)–CeMT2 isoform [16,20]. The slight blue-shift of the spectrum of Cd6Zn1–CeMT1 in relation tothat of Cd6–CeMT2 is attributable to the influence of the ... levelto be of the highest interest, in order to shed light on the possible physiological functions of MTs in thisorganism and to further the understanding of the forces driving MT isoform differentiation,...
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Báo cáo khoa học: A novel metallobridged bis(b-cyclodextrin)s fluorescent probe for the determination of glutathione doc

Báo cáo khoa học: A novel metallobridged bis(b-cyclodextrin)s fluorescent probe for the determination of glutathione doc

... which influences the flu-orescence intensity. Therefore, the influence of the vol-ume of buffer was measured. Because the volume of buffer added (1.00–3.00 mL) had little effect on the fluorescence ... fluorophores. The compound could be dis-solved in aqueous solution and showed high bindingability because of the two adjacent bis(b-CD)s.Because of the conformation of the linker of 1, the nitrogen ... analyticalsystem.Influence of pHBecause of the instability of CD and the amido bondat very low pH, the use of strongly acidic solution wasavoided [17]. Moreover, copper (II) will deposit inalkali solution. Thus the...
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Báo cáo khoa học: Cofactor-independent oxygenation reactions catalyzed by soluble methane monooxygenase at the surface of a modified gold electrode pot

Báo cáo khoa học: Cofactor-independent oxygenation reactions catalyzed by soluble methane monooxygenase at the surface of a modified gold electrode pot

... maintained.Detection of the products of substrate oxygenation by the adsorbed MMOH was achieved by holding the potential at)0.5 V for 30 min in the presence of substrate and thenremoving the liquid (50 ... fitted with GPES software. The surface of the 4-mm diameter gold electrode (OxfordElectrodes) was modified by cycling the electrode at reducing potentials in the presence of the hexapeptide, asdescribed ... forsubstrate. In the absence of substrate, MMOB can stimulateelectron transfer from the electrode to MMOH but it does not result in hydrogen peroxide formation because there isno substrate present....
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Báo cáo khoa học:

Báo cáo khoa học: "PCFGs, Topic Models, Adaptor Grammars and Learning Topical Collocations and the Structure of Proper Names" ppt

... consists of the set of trees with whose root node is labelled X andwhose ith child is a member of TBi.1149 The set of trees generated by the CFG is TS,where S is the start symbol, and the ... but ithas the advantage that the LDA distributions mapstraight-forwardly onto PCFG nonterminals). The terminals W of the CFG consist of the vo-cabulary V of the LDA model plus a set of special“document ... tree generated by such aCFG. The relationship between the LDA modeland the PCFG can be understood by studying the trees generated by the CFG. In these trees the left-branching spine of nodes...
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Báo cáo khoa học:

Báo cáo khoa học: "Adding Syntax to Dynamic Programming for Aligning Comparable Texts for the Generation of Paraphrases" potx

... (Milan Theater) and is not at the beginning of that constituent. Finally, the syntactic depen-dence trace of a word is the path of IOB valuesfrom the root of the tree to the word itself. The last ... used to guide the align-ment. Starting from the bottom right entry of the matrix, we go to the matrix entry from which the value of the current cell is derived in the recursion of the dynamic ... sentencesinstead of on their parse trees. Because of the se-mantic discrepancies among the inputs, applyingsyntactic features in the alignment has a larger im-pact on the grammaticality and fidelity of the...
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... function of man-ganese in the transcriptional regulation of the nrdoperon [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese ... to the catalytic site of the R1E subunit. Asreported in the Results, upon completion of substrateconversion, the radical is then rapidly passed backfrom the R1E subunit to the tyrosyl of the ... difference in the reactionmechanism of this RNR and that of other class 1RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E.We consider the first...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

... mark the boundary of the predicted substrate-binding pocket,reveals that some of them are conserved in the inac-tive isoform (i.e. Trp191 and Phe294). Even though the precise role of these ... human ACMSD. The presence of the metal-locofactor in human ACMSD might also be inferredby our study on the enzyme pH dependence, showingthat the increase of the concentrations of succinateand ... in the presence of increasing molar amounts of the other iso-form (1 : 1, 1 : 1000 and 1 : 10 000). Detection of ACMSD I was not influenced by the presence of up to10 000-fold molar excess of...
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Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt

Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt

... quantitative strategy to offset the lack orexcess of kinetic energy (as a measure of temperature)and its effect on enzyme activity.Alternatively, we know of a number of examples of qualitative strategies, ... considered in the context of the relevance for the performance of metabolic networks, because the theory of metaboliccontrol analysis states that enzyme properties (concen-trations, kinetics and thermodynamics) ... in their 3¢-UTR sequence bindingsites for specific proteins that increase ⁄ decrease the rate of poly(A) shortening, i.e. affect the lifetime of the mRNA [32], therefore it is likely that the...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... unable per se of regulating the expression of ICAM-1, and other immune recognition moleculesother than Nor3.2-reactive CD1d.To our knowledge, iron loading does not regulatemembers of the MHC class ... cytometry revealed that the inhibition of cellgrowth was due to a decrease in the percentage of cellsin the S and G2 ⁄ M phases of the cell cycle (Fig. 3B).In a total of seven separate determinations, ... compounds within the different endolysosomalcompartments [23].In this study, we examined whether iron loading of the liver epithelial cell line HepG2 influenced the expression of immune regulatory...
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