Báo cáo " Effect of Cr$^{3+}$ concentration on structural and optical properties of ZnAl$ 2$O$ 4$:Cr$^{3+}$ " ppt
Tài liệu Báo cáo " The effect of cobalt substitution on structure and magnetic properties of nickel ferrite " pptx
... room temperature under field of 5 kOe and this measurement with the increase of Co2+ concentration yields the monotonic increase of saturation magnetization (MS) and coercive field (HC). ... moment per formula of Ni1-xCoxFe2O4 and equivalently, an enhancement of magnetization [7].The dependence of saturation magnetization Ms as a function of Co2+ concentration is shown in ... the saturation magnetization of Ni-ferrite which may be due to the substitution of Ni2+ ions by Co2+ on the octahedral sties. Therefore, the increasing Co2+ concentration (x) on the octahedral...
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Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx
... inhibition of ITK activity compromises HIVinfection, gene expression and replication [62].Our group has recently evaluated the effect of proteasome inhibitors on HIV infection and ⁄ or replica-tion. ... Authors Journal compilation ª 2011 FEBSMINIREVIEWTEC family kinases in health and disease – loss -of- function of BTK and ITK and the gain -of- function fusions ITK–SYK and BTK–SYKAlamdar Hussain1,2,*, ... is capable of transforming NIH-3T3 cells [70]. In addition, we and others have demonstrated that the activation and plasma membrane localization of the fusion constructare dependent on phosphatidylinositol...
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Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf
... Apparent concentrationsratio(cell/medium)Dye concentration (µM) On ice + CCCPFig. 2. Effect of rhodamine dye concentrations on their uptake into K562 cells. K562 cells were incubated either on ... fluorescence was monitored continuously with an excitation wavelength of 555 nm and an emission wavelength of 575 nm. Inhibition of the NBD-Cl-sensitive efflux allowed the uptake of TMRM and its quenching ... rhodamine dyes, and possibly drugs such as doxorubicin, from achieving equilibration of their concentration in the cytoplasm with their concentration in the exter-nal medium. The protection provided...
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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... residue, and absorption of tyrosine at 275 nm(e275= 1400 m)1Æcm)1) was used to estimate the concentration of Ab in solution. In four separate puri-fication experiments, the concentration of ... E22K, E22Q and D23N), and the availability of a rapid and simpleexpression and purification protocol will facilitatelarge-scale investigations of the molecular determinants of aggregation and fibrillation. ... synthesized Ab1–40 and Ab1–42. Formation of fibrils by all peptides was concentration- dependent, and exhibited kinetics typical of a nucleation-dependent polymerizationreaction. Recombinant and synthetic...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
... mRNA and its own transcript. Overexpression of MCPIP in cellsactivated with LPS results in downregulation of theIL-1b transcript and silencing of MCPIP gene expressionhas an opposite effect on ... fastdegradation of IL-1b transcript independently of thepresence of AREs (Fig. 6B, lanes 2 and 5). It is possiblethat in the region located before the AREs, consisting of 343 bp of the 3¢ UTR of the ... cytosolic fraction, polyclonal antibodiesagainst MnSOD (Sigma) for the membrane fraction and monoclonal antibodies against b-actin (Sigma) for thecytoskeleton fraction. Secondary antibodies...
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Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx
... athorough understanding of not only their structures,but also of the energetics of their folding reactions and conformational transitions among different forms.The structure of human telomeric ... strand, allowing for the calculation of the equilibrium distribution of conformational forms.Standard methods using UV absorbance at a singlewavelength to monitor the thermal denaturation of nucleic ... NaCl consisted of threeexponentials with s values of 40–85 ms, 250–950 ms and 1.5–10.5 s. The folding rate constantsapproached limiting values with increasing cation concentration; in addition,...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... Materials and methods. Relative band intensities were recorded using a CCDcamera, and the concentrations of individual variants was calculatedfrom a calibration curve of known 44/750 concentrations. ... chro-matography and chromatofocusing, as described previously[21].ResultsExpression and secretion of truncated variants of GCPIITo analyze the contribution of individual domains of human GCPII ... lysates and conditioned media. The highest expression levels in theconditioned media were 10 lgÆmL)1for the 44/750 and 1/750 variants. A decrease of more than 80-fold in thesecretion of recombinant...
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Báo cáo khoa học: Residues affecting the chloride regulation and substrate selectivity of the angiotensin-converting enzymes (ACE and ACE2) identified by site-directed mutagenesis pot
... mm NaCl.Effects of CL1 and CL2 site mutations on thechloride sensitivity of tACE and ACE2In order to observe the effects of the various mutations on the chloride sensitivity of tACE and ACE2, ... result of substitution of the tACEresidues Pro407 and Pro519 with Glu398 and Ser511, and the resulting projection of their side-chains intothe location of this site. Consequently, ACE2 onlybinds ... wild-type tACE and ACE2chloride sensitivityFollowing the removal of chloride ions by extensivedialysis, the effect of increasing chloride concentration on the activities of wild-type tACE and ACE2...
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Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt
... After elution and concentration of theprotein solution containing the cellulase activity after nativePAGE, the solution was mixed with sample buffer for SDS/PAGE without reducing agent and incubated ... hilaris cellulase contained an ORF975 bp long, starting with an ATG codon at position 38 and ending with a TAA codon at position 1014. A poly(A) tail and typical polyadenylation signal were found. ... [LIV]-[LIVMFYWGA](2)-[DNEQG]-[LIVMGST]-x-N-E-[PV]-[RHDNSTLIVFY], was con-served in the deduced amino-acid sequence of P. hilariscellulase (Fig. 3). The conserved potential proton donor and Fig. 2. Substrate specificity and degradationproducts of P. hilaris...
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Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx
... the protein at highconcentrations.The present study describes a very effective method of isolation and purification of PEC and its photoactivea-subunit. Crystallization and preliminary X-ray ... improvingpurification protocols. As a result, several comparativestudies on the Z-andE-configurations of the intacta-subunit, and also on photoactive peptides originatingfrom nonspecific degradations of the ... with a protein concentration of 15–20 mgÆmL)1was irradiated with light of 571 and 503 nm inducing the E-andZ-configuration, respectively.The complete transfer into both configurations wasobtained...
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