... mitogen-activated protein kinases (MAPKs), but not of VEGFR-3, was induced by VEGF-A, whereas phos-phorylation of VEGFR-2, VEGFR-3 and MAPKs wasinduced by VEGF-C, as reported in a previous studyusing human ... ECs by magnet immunosorting using anti-Lyve-1 antibody. Scale bars ¼ 200 nm. (B) Western blotting revealed that Lyve-1-positiveuterine ECs maintained expression of Lyve-1, Prox-1 and VEGFR-3, indicating ... of the lox P-flanked b–geo gene (Fig. 1). The plasmidvector-free transgene was introduced into ESCs, and G418-resistant clones were selected. We next per-formed 5-bromo-4-chloro-3-indolyl-b-d-galactopyrano-side...