... dyes. As previously mentioned, the methane producing bacteria were inhibited or inactivated by the presence of dye, but at the same time the intermediate products were utilized by other groups ... specific methane yield and 59.6% production via aceticlastic methanogenesis (Melpai et al., 1998). Table 1 - Results of Batch decolorization of MO and RB4 by anaerobic treatment under mesophilic ... dye, viz., azo type represented by Methyl Orange (MO) and anthraquinone type represented by Reactive Blue 4 (RB4) was performed using digested sludge under mesophilic (35OC) and thermophilic...